Development and validation of a gas chromatographic method for the assay of memantine hydrochloride in pure and tablet dosage forms

Siddappa, K.; Metre, Mallikarjun; Tambe, Mahesh; Mallikarjun, K.; Chandrakanth, Reddy

doi:10.2298/fupct1101001s

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, HPLC coupled with mass spectrometer (MS) has been employed for quantitation of memantine in human plasma [18] and urine [19]. Other methods including gas chromatography [9,20,21], spectrofluorometry [22], and spectrophotometry [22,23] are reported for quality control of memantine in raw material and tablet formulations. This work aimed to develop a new and convenient derivatization procedure for memantine analysis using 4-(Nchloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulphonyl 2,1,3-benzoxadiazole (DBD-COCl) as a fluorescent probe (Figure 1) [24].…”

Section: Introductionmentioning

confidence: 99%

HPLC-Fluorescent Analysis of Memantine: An Investigation on Fluorescent Derivative Formation

Prapatpong¹,

Techa-In²,

Padungpuak³

et al. 2015

Journal of Chemistry

View full text Add to dashboard Cite

Memantine is an N-methyl-D-aspartate receptor antagonist recommended for treatment of Alzheimer’s disease. Due to the lack of chromophores/fluorophores in memantine molecule, this work focuses on a novel procedure for memantine-fluorescent derivative formation enabling it to be monitored by a fluorescence detector. 4-(N-Chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulphonyl 2,1,3-benzoxadiazole (DBD-COCl) was chosen as a fluorescent probe since the carbonyl chloride of DBD-COCl could easily react with the amine on memantine to form the fluorescent derivative. The derivatization was achieved at 5 : 1 of DBD-COCl and memantine, at 60°C for 50 min. Structure elucidation from mass spectrometry and infrared spectroscopy spectra confirmed the amide bond of memantine-DBD-COCl. The derivative was stable up to 24 h and could be monitored by high performance liquid chromatography (HPLC) coupled with a fluorescence detector using a VertiSep GES C18 column, acetonitrile and water (80 : 20) as the mobile phase with a flow rate of 1 mL/min using the excitation and emission wavelengths at 430 and 520 nm, respectively. The derivative was eluted at 4.50 min and was separated from the DBD-COCl. Preliminary validation data reveals good linearity (r2≥0.99), repeatability (%RSD < 0.54) and accuracy (%Rbetween 94 and 119%) with a limit of detection of 0.79 μg/mL.

show abstract

Section: Introductionmentioning

confidence: 99%

HPLC-Fluorescent Analysis of Memantine: An Investigation on Fluorescent Derivative Formation

Prapatpong¹,

Techa-In²,

Padungpuak³

et al. 2015

Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…There is also a published sequential injection analysis of memantine with chemiluminiscense [5]. Separation based techniques for memantine determination using GC-FID were suggested by Sidappa et al [6] and Jadav et al [7]. Regarding the separation techniques using liquid mobile phases, HPTLC method was published by Patel et al [8].…”

mentioning

confidence: 99%

Development of a novel, fast, simple, nonderivative HPLC method with direct UV measurement for quantification of memantine hydrochloride in tablets

Piponski¹,

Stoimenova²,

Stefov³

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

Fast, simple, accurate, and reproducible reverse phase‐high‐performance liquid chromatography method with direct ultraviolet measurement of memantine hydrochloride in tablets was developed, without any chemical derivatization pretreatment. Three main problems appear during chromatographic analysis of memantine: detection, achieving appropriate column retention, and limited choice of mobile phase components, as a result of memantine molecular structure. Among more than 35 tested columns, the best retention and peak symmetry yielded two C8 and three C18 columns with different characteristics, at a temperature of 30°C, mobile phase composed of 1%, v/v, acetonitrile and 99%, v/v, of 0.05–0.1% phosphoric acid or 2.5–5 mmol phosphate buffer, at flow rate of 1 mL/min and injection volume of 5 µL. The retention time of memantine was between 2.6 and 4 min. Both mobile phase concepts showed perfect linearity, precision, and accuracy. This is the first successful and reproducible direct reverse phase‐high‐performance liquid chromatography–ultraviolet quantification method for memantine.

show abstract

Investigations on molecular interactions of memantine hydrochloride in aqueous solutions by thermophysical methods and molecular dynamics simulations at different temperatures

Rayinuthala

Reddy

et al. 2023

Journal of the Indian Chemical Society

View full text Add to dashboard Cite

Development and validation of a gas chromatographic method for the assay of memantine hydrochloride in pure and tablet dosage forms

Cited by 4 publications

References 15 publications

HPLC-Fluorescent Analysis of Memantine: An Investigation on Fluorescent Derivative Formation

HPLC-Fluorescent Analysis of Memantine: An Investigation on Fluorescent Derivative Formation

Development of a novel, fast, simple, nonderivative HPLC method with direct UV measurement for quantification of memantine hydrochloride in tablets

Investigations on molecular interactions of memantine hydrochloride in aqueous solutions by thermophysical methods and molecular dynamics simulations at different temperatures

Contact Info

Product

Resources

About