Development and validation of a multianalyte method for quantification of mycotoxins and pesticides in rice using a simple dilute and shoot procedure and UHPLC-MS/MS

Silva, Lucas Pinto da; Madureira, Fernando Diniz; Vargas, Eugenia de Azevedo; Faria, Adriana Ferreira de; Augusti, Rodinei

doi:10.1016/j.foodchem.2018.07.126

“…However, some sorbents used for the cleanup step should be carefully used because it can remove some pesticides resulting in low recoveries (Perestrelo et al 2019). However, methods that use only one step of extraction, dispensing the cleanup step, have also been promisingly reported for rice (Silva et al 2019), and urine, serum, tomato, soil, commercial dosage form, and water samples (Gebrehiwot et al 2019). Lehotay (2019) reported that dispersive solidphase extraction (d-SPE) cleanup in QuEChERS method for determination of pesticide residues in fruits and vegetables helped reduce the amount of co-extracted matrix from the extracts and the matrix effect; however, the extent of cleanup was too small to justify this additional step.…”

Section: Introductionmentioning

confidence: 99%

Dilution of QuEChERS Extracts Without Cleanup Improves Results in the UHPLC-MS/MS Multiresidue Analysis of Pesticides in Tomato

Stringhini

¹

,

Ribeiro

²

,

Rocha

³

et al. 2021

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Tomato is well-known to be one of the most cultivated and consumed vegetables worldwide and frequently contain pesticide residues. Therefore, a simple multiresidue method was established and validated to determine 129 pesticides and metabolites in tomato samples using a modified acetate QuEChERS without cleanup for sample preparation and determination by ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Dilution of the raw extract in different proportions of mobile phase was evaluated and a dilution of 10 times presented adequate results improving analysis performance while minimizing the matrix effect. Validation performed according to SANTE guideline presented satisfactory results. Practical method limit of quantification was 0.01 mg kg−1 for most compounds. Recoveries between 70 and 120% with precision ≤ 20% were found for most compounds and spike levels evaluated. Matrix effect results were not significant for most part of compounds. Method proved to be simple, robust, and effective to be applied in routine analysis. Method applicability was performed by analysis of samples commercialized in Brazil and positive results were found demonstrating the importance of the proposed method.

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“…Despite the fact that the use of antibodies with electrochemical readouts was advantageous for achieving some of the lowest LODs for fumonisin B1, equivalent to 4.6e-7 and 3.7e-6 µg/L [101,109], these immunosensors were not included in the principal component analysis (PCA), as no assay time was reported in either case. Hence, as indicated in Figure 5, LC-MS [56,75], immunoassays with optical [99,107,135], Raman (due to its quick procedure) [140], fluorescent readouts [147] and electrochemical MIPs [191] were correlated to the combination of low LODs with short assay times. However, such statistical analysis did not show the advantages of aptamer-based methods, which was also observed on the correlation of short assay preparation times with LC-MS, immunologic and only three aptasensors [159,167,200].…”

Section: Conventional and Novel Methods For Mycotoxin Identificationmentioning

confidence: 99%

“…Sample clean-up is a key step for reducing matrix effect, where strong anion exchange (SAX) columns have been utilized as cheaper clean up cartridges in LC-MS detection, with recoveries of up to 86.6 and 106% for human hair [47] and piglet urine [56] respectively. In a similar way, immunoaffinity columns (IAC) have been proven to attain maximum recoveries of 109% for FMOCderivatized cornmeal samples [55], and 90% in rice analyzed by LC-MS [75]. The specificity of antibodies in IAC also allowed the successful LC-MS analysis of FB1 in complex samples, such as milk [43], human urine [44] and chicken tissue [80] with peak recoveries of 88.4%, 99.1% and 95-102% respectively.…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

“…On the other hand, one of the main advantages of mass spectrometry detectors is the possibility of performing multiplex analysis, not only for different mycotoxins [48,49,50,53,54,57,58,59,60,61,64,65,66,67,71,72,73,74,77,79,80,187], but also when combined with varied metabolites. Growth regulators, antibiotics, pesticides [51,70,75], and other fungal metabolites [63,68], were simultaneously identified in analysis capable of assessing up to 74 and 90 compounds [62,78]. Mass spectrometry has also been used for assessing mycotoxin transfer from feed to organs and tissue in poultry [80] The importance of novel analytical methods relies on the low sensitivities achieved within a relatively short detection time.…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

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Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

1

0

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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“…On the other hand, one of the main advantages of mass spectrometry detectors is the possibility of performing multiplex analysis, not only for different mycotoxins [48,49,50,53,54,57,58,59,60,61,64,65,66,67,71,72,73,74,77,79,80,187], but also when combined with varied metabolites. Growth regulators, antibiotics, pesticides [51,70,75], and other fungal metabolites [63,68], were simultaneously identified in analysis capable of assessing up to 74 and 90 compounds [62,78]. Mass spectrometry has also been used for assessing mycotoxin transfer from feed to organs and tissue in poultry [80] The importance of novel analytical methods relies on the low sensitivities achieved within a relatively short detection time.…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

¹

,

Gong²,

Goycoolea³

2020

Preprint

1

0

View full text Add to dashboard Cite

Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

show abstract

Development and validation of a multianalyte method for quantification of mycotoxins and pesticides in rice using a simple dilute and shoot procedure and UHPLC-MS/MS

Cited by 45 publications

References 7 publications

Dilution of QuEChERS Extracts Without Cleanup Improves Results in the UHPLC-MS/MS Multiresidue Analysis of Pesticides in Tomato

Dilution of QuEChERS Extracts Without Cleanup Improves Results in the UHPLC-MS/MS Multiresidue Analysis of Pesticides in Tomato

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

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