Development and validation of a rapid turboflow LC-MS/MS method for the quantification of LSD and 2-oxo-3-hydroxy LSD in serum and urine samples of emergency toxicological cases

Abstract

The validation data showed no significant matrix effects and no ion suppression has been 7 observed in serum and urine. Mean intraday accuracy and precision for LSD were 101% and 8 6.84%, in urine samples and 97.40% and 5.89% in serum, respectively. For O-H-LSD the 9 respective values were 97.50% and 4.99% in urine and 107% and 4.70% in serum. Mean interday 10 accuracy and precision for LSD were 100% and 8.26% in urine and 101% and 6.56% in serum. 11For O-H-LSD the respective values were 101% and 8.11% in urine and 99.8% and 8.35%, in 12 serum. The lower limit of quantification for LSD was determined to be 0.1 ng/ml. LSD 13 concentrations in serum were expected to be up to 8 ng/ml. 2-oxo-3-hydroxy LSD concentrations 14 in urine up to 250 ng/ml. 15The new method was accurate and precise in the range of expected serum and urine

Alterations of consciousness and mystical-type experiences after acute LSD in humans

“…The 0.5, 1.5, and 2.5 h samples were not collected in study 1. Blood samples were immediately centrifuged, and the plasma was rapidly stored at −20 °C and later analyzed using liquid-chromatography-tandem mass-spectrometry as previously reported (Dolder et al 2015a; Steuer et al 2016). Maximal plasma concentrations ( C max ) and total exposure (area under the plasma concentration-time curve [AUC]) were estimated using compartmental modeling in Phoenix WinNonlin 6.4 (Certara, Princeton, NJ, USA).…”

Simultaneous determination of LSD and 2-oxo-3-hydroxy LSD in hair and urine by LC–MS/MS and its application to forensic cases

“…Martin et al [14] investigated LSD and its metabolites in some suspected LSD intoxication cases, and LSD (below LOQ to 1.5 ng/ml) and O-H-LSD (0.8-2.3 ng/ml) were detected in samples from three suspects. Dolder et al [11] also analyzed some toxicological samples and identified LSD and O-H-LSD in urine at concentrations of 1.3 and 9.7 ng/ml, respectively. Urinary concentrations of LSD found in our authentic specimens are similar to those reported.…”

“…According to a study by Peel and Boynton, LSD and its metabolites were detectable in urine samples from patients receiving 300 g of LSD for 34-120 h [9]. Since simultaneous determination of the parent drug and its metabolites assist in the interpretation of analysis, recent studies have included both LSD and O-H-LSD as suitable biomarkers for LSD intake [10][11][12][13][14]. When the timely collection of urine from drug suspects is impossible, hair has been used as an alternative.…”

“…With improvements in instrumental techniques, analytical methods employing mass spectrometry have been developed for the detection of LSD or/and its metabolites at low concentrations in various biological matrices [10][11][12][13][14][15][16][17]. However, studies on the analysis of LSD and its metabolites in hair are limited in comparison with those in urine or blood.…”

A Single Dose of LSD Does Not Alter Gene Expression of the Serotonin 2A Receptor Gene (HTR2A) or Early Growth Response Genes (EGR1-3) in Healthy Subjects

“…The blood samples were immediately centrifuged, and the plasma was rapidly stored at -20°C and later at -80°C until analysis. LSD concentrations in plasma were determined using liquid-chromatography-tandem mass-spectrometry as reported in detail elsewhere (Dolder et al, 2015a; Steuer et al, 2016). The lower limit of quantification was 0.05 ng/ml (Dolder et al, 2015a).…”