Development and validation of a stability indicative HPLC–PDA method for kaurenoic acid in spray dried extracts of Sphagneticola trilobata (L.) Pruski, Asteraceae

Fucina, Giovana; Block, Luciana Catia; Baccarin, Thaisa; Ribeiro, T. R. G.; Quintão, Nara Lins Meira; Filho, Valdir Cechinel; Silva, Ruth Meri Lucinda; Bresolin, Tania Mari Bellé

doi:10.1016/j.talanta.2012.09.024

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…The samples were monitored at 210 and 338 nm. This methodology, previously developed and validated for S. trilobata spray-dried extract analysis, proved to be linear over a range of 4.5-30 lg/mL for KA, accurate (recovery of 99.0%), precise (the RSD % values for the intra-and inter-day precision studies were <2.0 and 8.0% for inter-laboratorial study), selective, stability-indicative and robust (Fucina et al 2012).…”

Section: Lc-uv Analysismentioning

confidence: 96%

“…TMB, TPA, croton oil, arachidonic acid (AA), hematoxyline, eosine, EDTA, HTAB (Sigma Aldrich, St. Louis, MO), formaldehyde, ethanol 100%, H 2 O 2 , acetone, xylene (Vetec, RJ, Brazil), NaCl, Na 2 HPO 4 , KH 2 PO 4 (Merck, Kenilworth, NJ). The chemical marker KA was isolated from S. trilobata roots with HPLC purity of >95% (Fucina et al 2012). Other solvents and reagents were of analytical grade and were purchased from national suppliers and commercially available.…”

Section: Drugs and Reagentsmentioning

confidence: 99%

“…The dried extract was obtained and the concentrate was mixed with 15% (w/w total solids of concentrate) of colloidal silicon dioxide and then dried in an industrial spray dryer (GEA-Niro, Søborg, Denmark) with inlet and outlet temperatures of 150-170 C and 70-80 C, respectively. Batches 1, 2 and 3 were analysed by a validated HPLC-UV method presenting 6.3, 5.9 and 6.1 mg/g of KA, respectively (Fucina et al 2012).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The published data demonstrate the presence of KA in all parts of the plant, however, high concentrations accumulate in the roots (Block et al 1998b;Bresciani et al 2000). The KA marker was quantified in S. trilobata spray-dried extracts by Fucina et al (2012); however, many peaks of the LC-UV chromatogram remain unidentified.…”

Section: Introductionmentioning

confidence: 99%

“…Our research group, in addition to morpho-anatomical (Baccarin et al 2009), phytochemical, efficacy (Block et al 1998a.b;Bresciani et al 2004) and oral safety studies (B€ urger et al 2005), has developed analytical methods to control the quality of S. trilobata hydroethanolic dried extracts (Fucina et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

Fucina¹,

Rocha²,

Silva³

et al. 2016

Pharmaceutical Biology

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Context: The aerial parts of Sphagneticola trilobata (L.) Pruski (Asteraceae) are popularly used to treat topical inflammation, but have not been fully investigated. Objective: To identify polar compounds in S. trilobata extracts and develop a new topical phytomedicine based on the kaurenoic acid (KA) content while monitoring and demonstrating its topical anti-inflammatory activity. Materials and methods: Ethanol spray-dried extract of S. trilobata was analysed by LC-MS while the KA content from semisolid was analysed by LC-UV. The extent of ear edema induced by applying 20 lL of croton oil (2.5%), arachidonic acid (AA; 2 mg/ear) and decanoylphorbol-13-acetate (TPA; 2.5 mg/ear) in mice was used to evaluate the biological activity of the semisolids, which were applied 30 min before the phlogistic agents. Results: Eight phenylpropanoids and four oleanane-type triterpenoid saponins were identified, majority of them reported for the first time in this species, in addition to KA. The semisolid containing 1.0% of dried extract reduced the ear edema induced by croton oil [77.2 6 4.5%; ID50 ¼ 0.49 (0.28-0.87%)], TPA (81.5 6 2.4%) and AA (39.1 6 6.9%), with decreasing effect at higher KA concentrations. This was accompanied by neutrophil migration inhibition as investigated by biochemical and histological assays. Discussion and conclusion: The anti-inflammatory effects were (at least in part) due to the interference in protein kinase C (PKC) activation, AA-cascade products and neutrophil migration inhibition, demonstrating the efficacy of the folk topical usage of this plant. The results support the development of a novel topical anti-inflammatory phytomedicine properly standardized to treat inflammatory dermatological diseases. ARTICLE HISTORY

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Section: Lc-uv Analysismentioning

confidence: 96%

Section: Drugs and Reagentsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

Fucina¹,

Rocha²,

Silva³

et al. 2016

Pharmaceutical Biology

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HPLC-PDA method validated for the determination of hibalactone in Hydrocotyle umbellata subterraneous parts and its ultrasound-assisted extraction optimization

Oliveira

Almeida

Oliveira

et al. 2019

Revista Brasileira de Farmacognosia

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Current initiatives for the validation of analytical methods for botanicals

Brown

Lister

2014

Current Opinion in Biotechnology

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The demand for validated analytical methods for botanicals has grown in response to the increasing consumer market for botanical supplements. Government initiatives to increase the availability of validated analytical methods and botanical reference material have led to the publication of numerous validation studies in scientific journals. Single laboratory validation and collaborative validation studies are structured to confirm a method's ruggedness and fit for purpose. The performance characteristics and statistical protocols followed throughout a validation study vary with the source of guidelines. Analytical techniques and priority methods are influenced by the need for fast-screening techniques, the limited availability of reference material, market value, and the prevalence of contaminants in botanical supplements.

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Development and validation of a stability indicative HPLC–PDA method for kaurenoic acid in spray dried extracts of Sphagneticola trilobata (L.) Pruski, Asteraceae

Cited by 13 publications

References 18 publications

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

HPLC-PDA method validated for the determination of hibalactone in Hydrocotyle umbellata subterraneous parts and its ultrasound-assisted extraction optimization

Current initiatives for the validation of analytical methods for botanicals

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