Development and validation of a High Performance Liquid Chromatography-Diode Array Detection method for the determination of aging markers in tequila

Muñoz-Muñoz, Ana Celia; Grenier, Adam Charles; Pulido, Humberto Gutiérrez; Cervantes-Martı́nez, Jesús

doi:10.1016/j.chroma.2008.10.018

Cited by 29 publications

(16 citation statements)

References 25 publications

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“…Three compounds, FUR, 2AF and 5MF were identified in each sample; no other peaks were observed in the 250-400 nm range. The identified compounds have been previously reported in the literature 9,12,32 and are commonly present as components of tequila and mezcal samples. Table 2 shows, in the HPLC column for each compound, the measured and mean concentrations expressed in mg per 100 mL AA.…”

Section: Hplc Methodsmentioning

confidence: 99%

Identification and quantification of furanic compounds in tequila and mezcal using spectroscopy and chemometric methods

Muñoz-Muñoz

Pichardo‐Molina²,

Ramos‐Ortíz³

et al. 2010

J. Braz. Chem. Soc.

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Neste artigo, duas bebidas mexicanas com denominação de origem controlada (DOC), tequila e mezcal, foram facilmente discriminadas por espectrofotometria UV-Vis e métodos quimiométricos. Os espectros foram registrados na faixa de 250-400 nm; a principal característica foi uma banda larga centrada em torno de 280 nm, cuja amplitude varia segundo o tipo de bebida e de marca. No entanto, diferenças adicionais foram identificadas através de análise multivariada, mostrando uma distinção clara entre as bebidas. Usando RP-HPLC com detector ultravioleta, os compostos que têm origem na banda de absorção foram determinados. Portanto, o espectro de cada bebida na faixa de comprimento de onda de 250-330 nm aproximadamente, mostrou uma mistura particular de furfural (FUR), 2-acetilfurano (2AF) e 5 methylfurfural (5MF). Além disso, os espectros de absorção das misturas de FUR, 2AF e 5MF foram registrados em diferentes concentrações e com um modelo de mínimos quadrados parciais (PLS), as concentrações desses compostos foram previstas em amostras de teste e em licores. Quando estes resultados foram comparados com os obtidos por HPLC, os coeficientes de correlação foram de R ≥ 0,920. Portanto, esta metodologia apresenta um método alternativo para identificar e quantificar alguns compostos furanos em tequilas brancas e mezcales. Além destes resultados, a metodologia pode ser realizada in situ e resultados específicos no controle de qualidade podem ser obtidos em poucos minutos.In this paper, two Mexican white spirits with appellation d'origen contrôlée (AOC), i.e., tequila and mezcal were easily discriminated by using UV-Vis spectrophotometry and chemometric techniques. The spectra were recorded in the range of 250-400 nm and their main feature is a broad band centered about 280 nm whose amplitude changes according to the type of beverage and brand, however, additional differences are pointed out through multivariate analysis that makes a fair differentiation between the beverages. By using RP-HPLC with UV detector, the compounds that originate the absorption band were determined. Thus, the spectrum of each beverage in the wavelength range of 250-330 nm, approximately, is a particular mixture of furfural (FUR), 2-acetylfuran (2AF) and 5-methylfurfural (5MF). Further, the absorption spectra of mixtures of FUR, 2AF and 5MF standards were recorded at several concentrations and by using a partial least square model (PLS), the concentrations of those compounds were predicted in test and spirit samples. When these results were compared to those obtained by HPLC, the correlation coefficients were R ≥ 0.920. Therefore, with this methodology one builds up an alternative method to identify and quantify some furanic compounds in white tequilas and mezcals. In addition to these results, the methodology can be performed on site and results about specific quality controls can be obtained within minutes.

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Section: Hplc Methodsmentioning

confidence: 99%

Identification and quantification of furanic compounds in tequila and mezcal using spectroscopy and chemometric methods

Muñoz-Muñoz

Pichardo‐Molina²,

Ramos‐Ortíz³

et al. 2010

J. Braz. Chem. Soc.

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“…2. (Mangas, Rodríguez, Moreno, Suárez, & Blanco, 1996;Muñoz-Muñoz, Grenier, Gutiérrez-Pulido, & Cervantes-Martínez, 2008). The statistical analysis (ANOVA) was performed to see if white and rested tequilas show statistical differences.…”

Section: Chemometric Studymentioning

confidence: 99%

Screening method for identification of adulterate and fake tequilas by using UV–VIS spectroscopy and chemometrics

Contreras

Barbosa-Garcı́a

Pichardo‐Molina

et al. 2010

Food Research International

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“…The LOD was calculated as LOD = 3.3 9 s/b, where s is the standard deviation for the y-intercept of the calibration curve and b is the slope of the calibration curve. LOQ was calculated as LOQ = 10 9 s/b (Munoz-Munoz et al, 2008).…”

Section: Dpph In Meohmentioning

confidence: 99%

Development of a spectroscopic method to determine the content of free radical scavenging compounds and oxidation products in thermally oxidised oils

Song

Jang

Kim

et al. 2016

Int J of Food Sci Tech

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Summary A simple spectroscopic method using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) was developed to simultaneously monitor the contents of free radical scavenging antioxidants (FRSs) and oxidised lipid products during oxidation through selection of proper solvents. Validation of the DPPH method was conducted in a stripped oil matrix, and practical applicability of the method was tested with heated oils. The absorbance of DPPH in isooctane simultaneously reflected the changing amounts of both FRSs and oxidised lipid products, whereas the absorbance in methanol mainly reflected changes in FRSs. Total polar materials (TPMs) were found to be representative of oxidised lipid products, which reacted with DPPH in isooctane better than in methanol. Validation parameters including accuracy, precision, linearity, limit of detection and limit of quantification were determined using α‐tocopherol as a model for FRSs and TPMs for oxidised lipid products. The DPPH protocol in isooctane and in methanol is useful for assessing the degree of oxidation in heated oils through comparing the contents of FRSs and oxidised lipids.

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Development and validation of a High Performance Liquid Chromatography-Diode Array Detection method for the determination of aging markers in tequila

Cited by 29 publications

References 25 publications

Identification and quantification of furanic compounds in tequila and mezcal using spectroscopy and chemometric methods

Identification and quantification of furanic compounds in tequila and mezcal using spectroscopy and chemometric methods

Screening method for identification of adulterate and fake tequilas by using UV–VIS spectroscopy and chemometrics

Development of a spectroscopic method to determine the content of free radical scavenging compounds and oxidation products in thermally oxidised oils

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