Development and validation of a microsatellite marker-based method for tracing infections by Microsporum canis

Abstract

Development and validation of a microsatellite marker-based method for tracing infections by Microsporum canis, Journal of Dermatological Science 70 (2) 123-129http://dx.doi.org/10.1016/j.jdermsci.2013.01.003You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy.

Molecular Epidemiology of Japanese Isolates of <i>Microsporum canis</i> Based on Multilocus Microsatellite Typing Fragment Analysis

“…Five episodes of infection of humans and cats living in the same household were analyzed, with the strains in all 5 respective households found to be of the same genotypes (Table 3). To evaluate inter-laboratory reproducibility, we analyzed a single M. canis strain, CBS113480 (14). The peaks for MS markers MS1, MS2, MS3, MS4, MS5, MS6, MS7, MS8, and McGT17 were consistently located at 118 bp, 106 bp, 117 bp, 112 bp, 106 bp, 115 bp, 122 bp, 117 bp, and 165 bp, respectively (Table 2).…”

“…In the initial application of MLMT to M. canis, 2 MS markers were used to identify 11 genotypes among 101 M. canis strains (13). Subsequently, MLMT using 8 MS markers was able to differentiate 22 genotypes among 26 M. canis strains (14). Moreover, strains derived from inter-individual infection episodes showed the same subtypes (14), indicating that MLMT is suitable for monitoring the in vivo transmission of M. canis.…”

“…regions using the enzymes MvaI (15) and HinfI (16). An additional strain, CBS113480, used in a previous MLMT study (14), was obtained from the CBS-KNAW Fungal Biodiversity Centre.…”

“…Among these markers, the MS8 nucleotide sequence (14) was found to be the same as that of McGT13 (13). Forward primers were labeled with one of the following fluorescent dyes: 6-FAM TM , VIC ® , PET ® , or NED TM (Applied Biosystems, Tokyo, Japan).…”

“…MS fragment analysis has recently been utilized in molecular epidemiology, with MS analysis of several different loci, called multilocus microsatellite typing (MLMT), possibly being the most sensitive method currently available for detection of intraspecies polymorphisms (10). MLMT has been used to genotype Trichophyton rubrum (11) and Candida albicans (12), and for the analysis of M. canis strains isolated from individuals with zoonosis (13,14). This study utilized MLMT to detect intraspecies polymorphisms of M. canis strains isolated from both humans and domesticated cats in Japan.…”