Development and validation of a new qualitative ELISA screening for multiresidue detection of sulfonamides in food and feed

Galarini, Roberta; Diana, Francesca; Moretti, Simone; Puppini, Barbara; Saluti, Giorgio; Persic, Lidija

doi:10.1016/j.foodcont.2013.07.014

Cited by 63 publications

(26 citation statements)

References 20 publications

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“…However, their widespread use may result in trace residues in products of animal origin such as milk, fish, eggs, meat, and honey, which attracts much attention of consumers and regulatory bodies due to potential adverse reactions including thyroid follicular tumors, allergy, as well as the development of antibiotic resistance 4 . To ensure food safety for consumers, EU Commission has laid down the maximum residue limits (MRL) of 100 µg kg -1 for SAs as a total in edible biological tissues, such as muscle, liver, kidneys, and milk 5 . No MRLs are fixed in honey samples, therefore, the measurable levels should be as low as reasonably achievable in these matrices.…”

Section: Introductionmentioning

confidence: 99%

“…Numberous analytical techniques have been employed for simultaneous determination of SAs in foodstuffs such as flow injection analysis 6 , adsorptive stripping voltammetry 7 , capillary electrophoresis 8,9 , electrochemical immunosensor 10 , enzyme-linked immunosorbent assays 5,11 , high-performance thin-layer chromatography 12 , and high performance liquid chromatography (HPLC) coupled with UV-Vis detector 13,14 , diode array detector (DAD) 15 , fluorescence detection (FLD) 16 , mass spectrometry (MS) 17,18 , and tandem mass spectrometry (MS/MS) 19 . Because of its high separation efficiency and low cost, HPLC is most widely applied to detect SAs among the high throughput analytical methods.…”

Section: Introductionmentioning

confidence: 99%

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NiMoO₄-modified MnO₂ hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

Zhang

Kong

et al. 2015

New J. Chem.

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3-(Trimethoxysilyl) propyl methacrylate-grafted sodium titanate nanotubes were synthesized and used as the monomer to functionalize poly(methacrylic acid-ethylene dimethacrylate) monolithic column. Various techniques, including scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, energy-dispersive X-ray spectrum, and thermal gravimetric analysis, were employed to characterize the synthesized monolith. The modified polymer monolithic column was further applied to the determination of sulfonamides in honey samples coupled to high performance liquid chromatography-diode array detector. Several experimental parameters affecting the extraction efficiency were investigated by single-factor experiments, such as sample pH, sample flow rate, sample volume, eluent flow rate, and eluent type. The first four factors were then further evaluated by means of a Taguchi's L 9 (3 4 ) orthogonal array experimental design. Under the optimized conditions, good linearities were obtained for all targets with squared regression coefficients values greater than 0.9937. The limits of detection (S/N = 3) and limits of quantitation (S/N = 10) were in the range of 0.68−1.42 and 2.28−4.72 µg kg -1 , respectively. The intra-day and inter-day relative standard deviations were determined to be from 1.9% to 6.9%. The recoveries of targets at different spiked levels ranged from 80.3% to 109.9% with relative standard deviations less than 9.5%, suggesting a promising prospect of the developed method in the application of real samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NiMoO₄-modified MnO₂ hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

Zhang

Kong

et al. 2015

New J. Chem.

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“…Sulphamethazine (SM 2 ) is broadly used in veterinary medicine to treat infectious diseases (Deng et al, 2016;Galarini et al, 2014;Wang et al, 2012). SM 2 abuse in veterinary practice may lead to the presence of SM 2 residues in milk; these residues are harmful to consumers because of their carcinogenic potential and risk of developing antibiotic resistance (Barani et al, 2015;Karageorgouet al, 2016;Marshall et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Song

Huang

Chen

et al. 2019

Food and Agricultural Immunology

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A novel lateral flow assay (LFA) was developed by introducing magnetic beads with colloidal gold nanoparticles (AuMB) to LFA test strips for the qualitative and quantitative detection of sulphamethazine (SM 2 ) in raw milk. In the proposed assay, test samples are qualitatively judged yes (positive) or no (negative) on the basis of naked-eye signals from the colloidal gold and fluorescence quenching is used to quantitatively determine the level of SM 2 in positive samples. Results indicated that the threshold concentration for determining whether a test sample is positive or negative is 5 ng/mL. The limit of detection and linear range of the proposed AuMB-LFA for quantitative detection were 0.39 and 0.5-200 ng/mL, respectively, and its recovery for detecting SM 2 in raw milk was 97.4-110.2%. The developed AuMB-LFA provides novel insights into efforts to maintain a balance between the efficient screening of targets in large samples and saving time and money. ARTICLE HISTORY

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“…Sulfamethazine (SM 2 ) is widely used in veterinary medicine for treatment of infectious diseases. The abuse of using SM 2 in veterinary practice may lead to the presence of SM 2 residues in milk (Franek, Kolar, Deng, & Crooks, 1999;Galarini et al, 2014;Karageorgou, Manousi, Samanidou, Kabir, & Furton, 2016;Wang et al, 2012). Excessive SM 2 residue is harmful to consumers because of its carcinogenic potential and risk of antibiotic resistance (Barani & Fallah, 2015;Marshall & Levy, 2011).…”

Section: Introductionmentioning

confidence: 99%

Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk

Song

Huang

et al. 2018

Food and Agricultural Immunology

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A novel lateral flow assay (LFA) was developed by introducing Eu (III)doped polystyrene nanoparticles (EuNPs) for rapid and ultra-sensitive detection of sulfamethazine (SM 2) in raw milk. The limit of detection and linear range of the proposed method were 0.0045 and 0.05-10 ng/mL, respectively. The recovery of LFA for the detection of SM 2 in raw milk was 96.1-108.2%. The proposed LFA provides a rapid and convenient strategy for fast and ultra-sensitive screening of SM 2 in raw milk. EuNP-LFA may be a remarkable method for the detection of other targets at low concentrations to ensure food safety.

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Development and validation of a new qualitative ELISA screening for multiresidue detection of sulfonamides in food and feed

Cited by 63 publications

References 20 publications

NiMoO₄-modified MnO₂ hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

NiMoO₄-modified MnO₂ hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk

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Development and validation of a new qualitative ELISA screening for multiresidue detection of sulfonamides in food and feed

Cited by 63 publications

References 20 publications

NiMoO4-modified MnO2 hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

NiMoO4-modified MnO2 hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk

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Product

Resources

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NiMoO₄-modified MnO₂ hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications

NiMoO₄-modified MnO₂ hybrid nanostructures on nickel foam: electrochemical performance and supercapacitor applications