Development and Validation of a Simple, Selective, and Accurate Reversed-Phase Liquid Chromatographic Method with Diode Array Detection (RP-HPLC/DAD) for the Simultaneous Analysis of 18 Free Amino Acids in Topical Formulations
Abstract:Even though there are reported methods for the quantification of free amino acids (FAAs) in biological products, no work has been done on the analysis of these substances in formulations. Moreover, further research is required as the reported methods do not fulfill analytical method requirements. The objective of this study was, therefore, to develop and validate a rapid, reliable, and appropriate RP-HPLC/DAD method for the simultaneous determination of 18 FAAs (l-Ala, l-Arg, l-Asn, l-Asp, l-Gln, l-Glu, l-Gly,… Show more
“…The acceptance criterion for this test was % interference of less than 5 at the retention time of the respective analytics. 12,13,19…”
Section: Methodsmentioning
confidence: 99%
“…The acceptance criterion for this test was % interference of less than 5 at the retention time of the respective analytics. 12,13, 19 3.4.4.2 Linearity and limit of detection and limit of quantication. Linearity was evaluated by the least-squares linear regression analysis by building a calibration curve according to the procedure described in section 2.4.3.…”
Rifampicin is an antibiotic effective against both Gram-negative and Gram-positive bacteria and is commonly used as a first-line treatment for tuberculosis caused by Mycobacterium tuberculosis. In this study, a reversed-phase...
“…The acceptance criterion for this test was % interference of less than 5 at the retention time of the respective analytics. 12,13,19…”
Section: Methodsmentioning
confidence: 99%
“…The acceptance criterion for this test was % interference of less than 5 at the retention time of the respective analytics. 12,13, 19 3.4.4.2 Linearity and limit of detection and limit of quantication. Linearity was evaluated by the least-squares linear regression analysis by building a calibration curve according to the procedure described in section 2.4.3.…”
Rifampicin is an antibiotic effective against both Gram-negative and Gram-positive bacteria and is commonly used as a first-line treatment for tuberculosis caused by Mycobacterium tuberculosis. In this study, a reversed-phase...
“…From (Fig. 3) shows that the analytical method gives a proportional linear response to the variation of analyte concentration which is indicated by the value of R 2 : 0.9998 with the regression equation y = 34706x + 83316 [24][25][26][27].…”
How to cite this article: Hariyanti H, Kurniati NF, Sumirtapura YC, Mauludin R. Development and validation of an analytical method for the determination of nanostructured lipid carrier's cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography.
“…36,57 For example, chromatographic methods are time-consuming and need advanced derivatization techniques. 58,59 Electrochemical procedures are constrained by molecular interference, and the detection of the biomolecules is sometimes hindered by photobleaching, which is a typical occurrence among uorescent probes. [60][61][62] Colorimetric tests are particularly notable among these approaches due to their inherent simplicity, high sensitivity, and cost-effectiveness.…”
Biomolecules play vital roles in many biological processes and diseases, making their identification crucial. Herein, we present a colorimetric sensing method for detecting biomolecules like cysteine (Cys), homocysteine (Hcy), and...
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