Development and validation of a molecular tool for assessing triploidy in turbot (Scophthalmus maximus)

Hernández‐Urcera, Jorge; Vera, Manuel; Magadán, Susana; Pino‐Querido, Ania; Cal, Rosa; Martı́nez, Paulino

doi:10.1016/j.aquaculture.2011.11.039

Cited by 17 publications

(15 citation statements)

References 40 publications

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“…After the induction of triploidy, confirmation of success is required. In order to confirm the ploidy number, techniques commonly used for fish species include flow cytometry (Lecommandeur et al ; Fujimoto et al ), cytogenetic analysis (Zhou et al ), blood smears (Gao et al ), and molecular biology (Hernandez‐Urcera et al ). As each procedure presents a particular advantage (Piferrer et al ), a combination of these techniques for triploid confirmation in A. altiparanae ensured the accuracy of our protocol.…”

Section: Discussionmentioning

confidence: 99%

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Adamov

Nascimento

Maciel

et al. 2016

J World Aquaculture Soc

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Triploidization is an interesting tool to produce sterile fish. In the yellowtail tetra, Astyanax altiparanae, this can be applied for aquaculture and surrogate technologies. In this study, we compared the efficacy of cold (2 C) or heat shock (38 C, 40 C, and 42 C) on triploid induction in the yellowtail tetra. The eggs were treated with cold or heat shock, 2 min postfertilization (30 min in cold shock or 2 min in heat shock). Intact embryos served as the control group. Ploidy status was confirmed by karyotyping, flow cytometry, and nuclear diameter of erythrocytes. The hatching rate decreased after cold shock (12.69 ± 15.76%) and heat shock at 42 C (0.35 ± 0.69%) in comparison with the control group (63.19 ± 16.82%). At 38 C and 40 C, hatching rates (61.29 ± 17.73% and 61.75 ± 22.1%, respectively) were not decreased. Only one triploid arose at 38 C (1/80). At 40 C, a high number of triploids arose (72/78). At 42 C, very few embryos developed into the hatching stage. A large number of haploid individuals arose after cold shock (61/75), with only one triploid. Our results indicate that heat shocking of embryos at 40 C is optimum for triploid production in the yellowtail tetra.

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Section: Discussionmentioning

confidence: 99%

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Adamov

Nascimento

Maciel

et al. 2016

J World Aquaculture Soc

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“…Individual salmon displaying three alleles at only one locus were not reported as triploid (this only occurred for two individuals and thus does not influence the results of the present study). Thus, the approach we have implemented to identify the frequency of triploids is identical to a recent study documenting spontaneous triploids in cultured Atlantic salmon in a Baltic fish hatchery [ 41 ], and is similar to the approach used to identify triploids in a variety of other fish and insect studies of triploidy [ 52 - 55 ]. Finally, these genetic markers in this laboratory have been used to extensively conduct parentage testing for Atlantic salmon in common-garden experiments [ 40 , 56 - 58 ].…”

Section: Methodsmentioning

confidence: 97%

“…Triploid organisms are readily identified through microsatellite DNA genotyping as they often display three clearly identifiable alleles per locus [ 52 ] (Additional file 1 ). However, not all loci will display trisomy in a triploid individual.…”

Section: Methodsmentioning

confidence: 99%

The frequency of spontaneous triploidy in farmed Atlantic salmon produced in Norway during the period 2007–2014

Glover¹,

Madhun²,

Dahle³

et al. 2015

BMC Genet

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BackgroundSpontaneous triploidy has been reported in a number of fish species, and is often linked with in vivo or in vitro ageing of eggs post ovulation. Here, we provide the first investigation into the frequency of spontaneous triploidy in farmed Atlantic salmon by analysing more than 4000 fish from 55 farms, and approximately 1000 recaptured escapees, all sampled in the period 2007–2014. In addition, we compare microsatellite genotyping against flow cytometry and red blood cell diameter in a set of 45 putatively diploid and 45 putatively triploid Atlantic salmon.ResultsThe three methods implemented for ploidy determination gave consistent results, thus validating the methods used here. Overall, 2.0% spontaneous triploids were observed in salmon sampled on farms. The frequency of spontaneous triploids varied greatly among sea cages (0-28%), but they were observed in similar frequencies among the three primary breeding companies (1.8-2.4%). Spontaneous triploids were observed in all farming regions in Norway, and in all years sampled. Spontaneous triploids were also observed among the escapees recaptured in both the marine environment and in rivers.ConclusionsSpontaneous triploidy in commercially produced Atlantic salmon is likely to be a result of the practices employed by the industry. For logistical reasons, there is sometimes a pause of hours, and in some cases overnight, between killing the female broodfish, removal of her eggs, and fertilization. This gives the eggs time to age post ovulation, and increases the probability of duplication of the maternal chromosome set by inhibition of the second polar body release after normal meiosis II in the oocyte.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-015-0193-0) contains supplementary material, which is available to authorized users.

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“…Candidate parents were excluded if a mismatch occurred at one or more of the loci. For the analysis of triploid fish, the two maternal alleles were coded as a single allele as detailed in Hernández‐Urcera, Vera, Magadán, Pino‐Querido, Cal and Martínez ().…”

Section: Methodsmentioning

confidence: 99%

Growth and gut morphology of diploid and triploid juvenile Atlantic cod (Gadus morhua )

et al. 2014

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13The objective of this paper was to compare the growth and gut morphology of 14 juvenile diploid and triploid Atlantic cod (Gadus morhua) reared under similar 15 conditions. Individually tagged 36-week old diploid (mean weight 49.3 ± 13.8 g) 16 and triploid (mean weight 43.6 ± 11.2) juvenile cod were measured at intervals 17 during a 29-weeks growth trial. Data for weight, length, condition factor (K),

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Development and validation of a molecular tool for assessing triploidy in turbot (Scophthalmus maximus)

Cited by 17 publications

References 40 publications

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

The frequency of spontaneous triploidy in farmed Atlantic salmon produced in Norway during the period 2007–2014

Growth and gut morphology of diploid and triploid juvenile Atlantic cod (Gadus morhua )

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