Development and validation of a multiclass method for the quantification of veterinary drug residues in honey and royal jelly by liquid chromatography–tandem mass spectrometry

Yugan, Jin; Zhang, Jinzhen; Zhao, Wen; Zhang, Wenwen; Lin, Wenxiong; Zhou, Jinhui; Li, Yi

doi:10.1016/j.foodchem.2016.11.026

Cited by 80 publications

(40 citation statements)

References 31 publications

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“…The presented calculated LOQ values are adequately precise, although lower values of 0.76 and 0.42 μg/kg were obtained for tylosin and lincomycin in Zhang et al (), owing to the much better sensitivity of UPLC‐QTOF‐MS compared with LC‐MS/MS. Note that the LOQs for lincomycin and tylosin A in the Jin et al () study performed using LC‐MS/MS were considerably higher, 12.68 and 3.55 μg/kg, respectively. The lower LOQ and LOD values obtained in this study demonstrate the outstanding efficiency of the proposed sample preparation method.…”

Section: Resultsmentioning

confidence: 86%

“…A literature search yields only a couple of articles on the determination of lincomycin and tylosin in royal jelly (Jin et al, ; Zhang et al, ). It should be noted that the definition of the residue level for tylosin in the Japanese Positive List System for Agricultural Chemical Residues in Foods (Ministry of Health, Labour and Welfare, Japan, ) refers to the sum of both tylosin A and tylosin B; however none of the aforementioned studies accounted for the presence of tylosin B.…”

Section: Introductionmentioning

confidence: 99%

“…The reference standard is now available, and studies on the determination of tylosin B are required. For this purpose, analytical methods such as ultra‐performance liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF‐MS) (Zhang et al, ) and liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC‐MS/MS) (Jin et al, ) have been used. Both studies have used the environmentally friendly and widely accepted quick, easy, cheap, effective, rugged, safe (QuEChERS) method; however, we could not obtain satisfactory recoveries, as reported in these studies.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

Zheng

Park

El‐Aty

et al. 2017

Biomedical Chromatography

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We have developed an analytical method for the determination of lincomycin, tylosin A and tylosin B residues in royal jelly using liquid chromatography-triple quadrupole tandem mass spectrometry analysis. For extraction and purification, we employed 1% trifluoroacetic acid and 0.1 m Na EDTA solutions along with an Oasis HLB cartridge. The target antibiotics were well separated in a Kinetex EVO C reversed-phase analytical column using a combination of 0.1% formate acid in ultrapure water (A) and acetonitrile (B) as the mobile phase. Good linearity was achieved over the tested concentration range (5-50 μg/kg) in matrix-matched standard calibration. The coefficients of determination (R ) were 0.9933, 0.9933 and 0.996, for tylosin A, tylosin B and lincomycin, respectively. Fortified royal jelly spiked with three different concentrations of the tested antibiotics (5, 10 and 20 μg/kg) yielded recoveries in the range 80.94-109.26% with relative standard deviations ≤4%. The proposed method was applied to monitor 11 brand of royal jelly collected from domestic markets and an imported brand from New Zealand; all the samples tested negative for lincomycin, tylosin A and tylosin B residues. In conclusion, 1% trifluoroacetic acid and 0.1 m Na EDTA aqueous solvents combined with solid-phase extraction could effectively complete the sample preparation process for royal jelly before analysis. The developed approach can be applied for a routine analysis of lincomycin, tylosin A and tylosin B residues in royal jelly.

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Section: Resultsmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

Zheng

Park

El‐Aty

et al. 2017

Biomedical Chromatography

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“…Previously reported extraction procedures for the detection of some of target antibiotics discussed herein from animal tissue and honey matrices have included pressurized liquid extraction (Carretero, Blasco, & Picó, ), liquid–liquid extraction (Martos et al, ) and QuEChERS (quick, easy, cheap, effective, rugged, and safe) (Jin et al, ). However, solid‐phase extraction (SPE) methods are most widely used to determine antibiotics because such methods afford satisfactory accuracy and precision (Bohm et al, ; Khong, Hammel, & Guy, ; Lopez, Pettis, Smith, & Chu, ).…”

Section: Introductionmentioning

confidence: 99%

“…QuEChERS (quick, easy, cheap, effective, rugged, and safe) (Jin et al, 2017). However, solid-phase extraction (SPE) methods are most widely used to determine antibiotics because such methods afford satisfactory accuracy and precision (Bohm et al, 2012;Khong, Hammel, & Guy, 2005;Lopez, Pettis, Smith, & Chu, 2008 In addition, solutions of 0.1 M Na 2 EDTA in distilled water, 2% TFA in distilled water and 0.02% ammonium in methanol were prepared for use in the extraction process.…”

mentioning

confidence: 99%

Development and validation of a solid‐phase extraction method coupled with LC–MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat

Zheng

El‐Aty

Kim

et al. 2019

Biomedical Chromatography

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The present study was carried out to determine 16 antibiotics belonging to seven different groups (tetracyclines, sulfonamides, penicillins, fluoroquinolones, macrolides, lincosamides and trimethoprims) in duck meat. A solid‐phase extraction method based on Oasis HLB cartridges coupled with liquid chromatography–electrospray ionization tandem mass spectrometry was developed. Solutions of 0.1 m ethylenediaminetetraacetic acid disodium salt and 2% trifluoroacetic acid were used for the preliminary extraction of the target antibiotics from duck meat and n‐hexane was used for purification prior to solid‐phase extraction. Mobile phases composed of 0.1% trifluoroacetic acid in distilled water (solvent A) and 0.1% trifluoroacetic acid in methanol (solvent B), combined with a reversed‐phase C18 analytical column, provided the optimal separation and signal intensity. The linearity of the method was assessed using six concentrations (5, 10, 20, 30, 40, and 50 μg/kg), and the recoveries, which were calculated at three spiking concentrations (5, 10 and 20 μg/kg), were in the range 69.8–103.3% with relative standard deviations (RSDs) ≤ 6.9% for the 16 tested antibiotics. Matrix effects ranging from −47.2 to −13.5% were observed for all the analytes, and the limits of quantitation (LOQ), which ranged from 4.93 to 26.21 μg/kg, were much lower than the maximum residue limits (MRLs) set by various regulatory authorities. Ten samples from a market were tested, and none of the target analytes were detected. Thus, a simple and versatile protocol has been developed to detect and quantify 16 antibiotics in duck meat samples.

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Graphene particles supported on silica as sorbent for residue analysis of tetracyclines in milk employing microextraction by packed sorbent

et al. 2018

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This paper describes the use of graphene-based sorbents for determination of four tetracyclines in milk. The synthesized materials were combined with microextraction by packed sorbent (MEPS) to act as the sample preparation step. The extraction performance of these sorbents was compared to commercial phases, and graphene supported on silica provided the best results. The analytical method optimization was carried out by employing experimental design. Firstly, an evaluation of the experimental variables (elution solvent, use of EDTA, ionic force, and pH of the washing solution) was made by a 2 factorial experimental design. The variables sampling, washing and elution cycles of MEPS were further optimized under a full 2 experimental design. The validation parameters were determined under optimized conditions resulting in a linearity ranging from 15 to 110 μg/L with R values above 0.98, and LOQs ranging from 0.05 to 0.9 μg/L. The accuracy ranged from 87.9 to 118.4% and intra/inter-day precision reported by the RSDs were lower than 19%. The proposed and validated method was successfully applied to the analysis of 11 milk samples from different animals, revealing traces of tetracyclines in only two of them. This study focused on the evaluation of graphene-based sorbents combined with MEPS for tetracycline analysis provided equivalent or even better results than other proposed methods, suggesting being a sensitive, fast and reliable alternative method for the determination of tetracyclines in milk samples.

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Development and validation of a multiclass method for the quantification of veterinary drug residues in honey and royal jelly by liquid chromatography–tandem mass spectrometry

Cited by 80 publications

References 31 publications

Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

Development and validation of a solid‐phase extraction method coupled with LC–MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat

Graphene particles supported on silica as sorbent for residue analysis of tetracyclines in milk employing microextraction by packed sorbent

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