Development and validation of a multiplex electrochemiluminescence immunoassay to evaluate dry eye disease in rat tear fluids
Agnese Compagnone,
An Matheeussen,
Linda De Vooght
et al.
Abstract:Dry eye disease (DED) is a challenge in ophthalmology. Rat models represent valuable tools to study the pathophysiology and to develop novel treatments. A major challenge in DED research is detecting multiple biomarkers in a low tear volume sample. Multiplex immunoassays for DED rat research are missing. We have developed a multiplex electrochemiluminescence immunoassay (ECLIA) to detect three biomarkers for DED: MMP-9, IL-17 and ICAM-1. Tears, used as matrix, were collected from six healthy Wistar rats. Assay… Show more
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