Development and validation of a rapid HPLC method for multiresidue determination of erythromycin, clarithromycin, and azithromycin in aquaculture fish muscles

Abstract

An accurate, sensitive, and reproducible high-performance liquid chromatographic method with diode array detection has been developed for simultaneous determination of erythromycin, clarithromycin, and azithromycin residues in fish muscles. Analysis was carried out using a Shodex Asahipak high-performance liquid chromatography (HPLC) column, monitoring was at 210 nm and a mobile phase consisting of a mixture of acetonitrile and phosphate buffer (pH 11 ± 0.05) in the ratio of 60:40 (v/v). Solid-phase extraction method was used in samples extraction and purification. Recoveries were in the range 72.0-92.2% with relative standard deviation (RSD) from 2.3% to 8.3%. This method was validated for fish muscles in aquaculture following the commission decision 2002/657/EC criteria. It is demonstrated that the new method is robust for detection and quantification of the three macrolides residues. Decision limit (CCα) was from 214 to 228 μg/kg and capacity of detection (CCβ) was from 228 to 256 μg/kg.

Sodium alginate and pectin estimation in raft forming pharmaceuticals by high performance liquid chromatography method

“…The specificity of the method was analyzed and no interference from the polymeric materials (sodium alginate and pectin) was observed by performing separate injections of standard, placebo and polymeric samples respectively. The obtained results are in agreement with previously reported results by Habibi et al in 2019 [10]. The retention time of the sodium alginate and pectin were 3.931 and 7.470 min respectively, as shown in Fig.…”

Determination of Multiclass Antibiotics in Fish Muscle Using a QuEChERS-UHPLC-MS/MS Method

“…In terms of quantitative determination, antibiotics are typically studied using liquid chromatography (LC) in combination with various detectors, including ultraviolet [19][20][21], fluorescence (FLD) [22,23] and diode array detectors (DAD) [24][25][26], or tandem mass spectrometry (MS/MS) [27][28][29] to name a few. As an example, Habibi et al [30] developed a method employing HPLC-DAD for the rapid and straightforward analysis of three macrolides in the muscle tissue of aquaculture fish (sea bream). This method has been applied to monitor products from Tunisian aquaculture.…”

Preparation and Application of Molecularly Imprinted Monolithic Extraction Column for the Selective Microextraction of Multiple Macrolide Antibiotics from Animal Muscles

“…Besides, spiramycin (SPM), tylosin (TYL), and tilmicosin (TIM), which were used as animal growth promoters before, are banned or severely restricted [9,10]. Worryingly, MALs, including TYL, TIM, erythromycin (ERY), and roxithromycin (ROX), are still frequently detected in animal foodstuffs due to their wide availability and low cost [11,12,13]. Thus, it is necessary to supervise MALs residues in animal-derived food.…”