Development and validation of a HILIC-UV method for the determination of nucleotides in fish samples

Logotheti, Maria; Theochari, Konstantina; Kostakis, Marios; Pasias, Ioannis N.; Thomaidis, Nikolaos S.

doi:10.1016/j.foodchem.2017.12.040

Cited by 15 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The degradation of ATP is an autolytic change, which is accompanied by muscle softening during the stiffness process and results in a decrease in fish freshness. The sequence of ATP degradation to Hx is as follows: ATP→ ADP→ AMP→ IMP→ HxR→ Hx (41). The initial K value was found to be 11.72% (Figure 3), and the muscle of turbot is fresh, which was similar to the previously reported value of turbot (42).…”

Section: K Value Resultssupporting

confidence: 86%

Shelf-Life Extension of Refrigerated Turbot (Scophthalmus maximus) by Using Weakly Acidic Electrolyzed Water and Active Coatings Containing Daphnetin Emulsions

et al. 2021

View full text Add to dashboard Cite

This research was to investigate the effect of weakly acidic electrolytic water (WAEW) treatments combining with the locust bean gum (LBG) and sodium alginate (SA) active coatings, containing daphnetin emulsions on microbiological, physicochemical, and sensory changes of turbot (Scophthalmus maximus) during refrigerated storage at 4°C for 24 days. Results showed that WAEW, together with LBG-SA coatings containing daphnetin emulsions treatments, could significantly lower the total viable count (TVC), H2S-producing bacteria, pseudomonas spp., and psychrotrophic bacteria counts, and inhibit the productions of off-flavor compounds, including the total volatile basic nitrogen (TVB-N), inosine (HxR), and hypoxanthine (Hx). Furthermore, the treatments also prevented textural deterioration, delayed water migration, and had higher organoleptic evaluation results. Therefore, WAEW, together with LBG-SA coatings, containing daphnetin emulsions treatments, had the potential to improve the quality of turbot during refrigerated storage.

show abstract

Section: K Value Resultssupporting

confidence: 86%

Shelf-Life Extension of Refrigerated Turbot (Scophthalmus maximus) by Using Weakly Acidic Electrolyzed Water and Active Coatings Containing Daphnetin Emulsions

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Nucleotides degradation is a biochemical change coinciding with muscle softening and ultimately with the quality decrease, which was usually monitored as a means to assess the freshness of aquatic products after organism’s death (Logotheti et al ., 2018). In previous studies, nucleotides catabolism monitoring has been mainly used in fish storage to reflect the freshness (Hong et al ., 2017), and barely applied to freshwater shrimp.…”

Section: Introductionmentioning

confidence: 99%

Effects of superchilling on quality of crayfish (Procambarus clarkii): water migration, biogenic amines accumulation, and nucleotides catabolism

Qin

Chen

et al. 2021

Int J of Food Sci Tech

View full text Add to dashboard Cite

Effects of refrigerated (3.5 AE 0.5 °C), iced (0 AE 0.5 °C) and superchilled (−3.0 AE 1.0 °C) storage on quality of crayfish (Procambarus clarkii) were evaluated by monitoring the water migration, microbial contamination, biogenic amines accumulation, and nucleotides catabolism during 21 days. The results indicated that superchilling was effective in accelerating the rate of water migration and reducing total volatile basic nitrogen, trimethylamine, total viable bacteria, Enterobacteriaceae and psychrotrophic bacteria of crayfish. Meanwhile, significant (P < 0.05) lower putrescine, cadaverine and tyramine contents were observed in superchilled sample (SS), whereas histamine concentrations were at lower levels (0.76-2.31 μmol g −1 ) in all treatments. In addition, the predominant nucleotides degradation pathway in crayfish was the conversion of inosine monophosphate, which was accumulated massively (3.74 µmol g −1 ) in SS at the end of storage. According to the dynamic changes of inosine and hypoxanthine contents, the shelf life of SS was prolonged to 15 days.

show abstract

“…3 High levels of xanthine in human biological fluids (≳4 μM in serum, ≳17 μM in urine) have also been linked to increased risk of leukemia and kidney stones, 3 hence quantitative determination of xanthine is also of clinical importance. Classical analytical methods such as chromatography coupled with spectroscopy or mass spectrometry detections 4,5 and enzyme-linked immunosorbent assays 3 have high xanthine sensitivity and reliability. However, these techniques tend to be timeconsuming, require skilled personnel, and are not suitable for in-field analysis.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Determination of Xanthine Using Nanostructured NiO Electrodes Loaded with Xanthine Oxidase

Tripathi

Elias

Jemere

et al. 2022

ACS Food Sci. Technol.

View full text Add to dashboard Cite

Nickel oxide (NiO) thin films prepared by glancing angle deposition (GLAD) were investigated as electrodes for enzymatic electrochemical quantification of xanthine, a noted indicator of meat freshness. The large surface area of the macroporous GLAD NiO electrodes provided a suitable scaffold to successfully immobilize the enzyme xanthine oxidase (XO), and this XO immobilization was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and X-ray photoelectron spectroscopy. The XO-modified GLAD NiO electrodes electrochemically oxidize xanthine, with electron transfer from this adsorbed XO to the (Ni2+/Ni3+) redox species resulting in a strong amperometric response to xanthine in a reagent-free alkaline medium. Under optimal conditions, the fabricated xanthine biosensor exhibited a rapid response (∼7 s), wide dynamic range (0.1–650 μM), good reproducibility (relative standard deviation of ∼4%, n = 18), superior limit of detection (37 nM), and very high sensitivity (1.1 μA·μM–1·cm–2 in the low concentration range from 0.1–5 μM and 0.3 μA·μM–1·cm–2 in the higher concentration range from 5–650 μM). The biosensor was also evaluated against a selection of potential interferents commonly found in fish samples (hypoxanthine, uric acid, glucose, and sodium benzoate), demonstrating good selectivity toward xanthine. A low Michaelis–Menten constant (K m) of 0.4 mM for xanthine signifies the high affinity of the enzymatic sensor toward the target analyte. Measurements in real fish samples were also successfully performed, revealing strongly increased xanthine sensitivity in the presence of fish matrices (from 0.085 μA μM–1 without fish extract to as much as 0.27 μA μM–1).

show abstract

Development and validation of a HILIC-UV method for the determination of nucleotides in fish samples

Cited by 15 publications

References 27 publications

Shelf-Life Extension of Refrigerated Turbot (Scophthalmus maximus) by Using Weakly Acidic Electrolyzed Water and Active Coatings Containing Daphnetin Emulsions

Shelf-Life Extension of Refrigerated Turbot (Scophthalmus maximus) by Using Weakly Acidic Electrolyzed Water and Active Coatings Containing Daphnetin Emulsions

Effects of superchilling on quality of crayfish (Procambarus clarkii): water migration, biogenic amines accumulation, and nucleotides catabolism

Amperometric Determination of Xanthine Using Nanostructured NiO Electrodes Loaded with Xanthine Oxidase

Contact Info

Product

Resources

About