“…The types of ELISAs include indirect, sandwich, direct and competition ELISAs [ 71 ]. Currently, ELISAs are commonly used immunoassays in human and veterinary medicine [ 72 ]. According to existing standard procedures, various kits can be developed for large-scale detection of substances with the help of scientific instruments.…”
Section: Methods Of Neutralizing Antibody Detectionmentioning
“…The types of ELISAs include indirect, sandwich, direct and competition ELISAs [ 71 ]. Currently, ELISAs are commonly used immunoassays in human and veterinary medicine [ 72 ]. According to existing standard procedures, various kits can be developed for large-scale detection of substances with the help of scientific instruments.…”
Section: Methods Of Neutralizing Antibody Detectionmentioning
“…We slowly added the mixture to the BSA solution (2 mg mL −1 ) and stirred at room temperature for 4–6 h. Following dialysis for 3 d, we obtained the immunogen, which was stored at −20 °C. 17,18 DQ-1 and DQ-2 were each coupled to ovalbumin (OVA) (2 mg mL −1 ) to generate the coating antigens. We used an American Agilent Cary 60 UV-vis spectrophotometer.…”
Residues of diquat (DQ), a bipyridyl herbicide, can pose a threat to human health. In this study, we designed two semi-antigens and obtained a highly specific monoclonal antibody (mAb) against...
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