Development and Validation of a Method for Determination of 43 Antimicrobial Drugs in Western-Style Pork Products by UPLC-MS/MS with the Aid of Experimental Design

Abstract

Western-style pork products have attracted many modern urban consumers, and these products have rapidly entered the Chinese market. The current hazard analysis of processed meat products mainly focuses on processing hazards (PAHs, microorganisms, and food additives), with less attention to veterinary drug residues. According to the survey results, the residues of antimicrobial drugs (sulfonamides and quinolones) in pork and its products in China are a severe problem, which may cause metabolic reactions, toxic effects, or enhance drug resistance. This study applied a modified QuEChERS method combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MSMS) to develop a rapid and sensitive method for determining antimicrobial drugs in bacon and ham was successfully evaluated methodologically by EU 2002/657/EC. This study used a three-level, three-factor Box–Behnken design (BBD) to optimize the QuEChERS method by response surface methodology. The excellent linearity of the calibration curve was shown in the corresponding concentration range with a coefficient of determination greater than 0.99. The values of decision limit (CCα) and detection capability (CCβ) were in the range of 10.9–31.3 μg/kg and 11.8–52.5 μg/kg, respectively. The method successfully detected two trace levels of antimicrobial drugs in commercially available samples, including sulfadiazine and moxifloxacin.

Assessing the performance of various sorbents in micro-solid phase extraction cartridges for pesticide residue analysis in feed

“…Various sorbents, including Z-sep and EMR-lipid, were used in different applications for the analysis of pesticides and veterinary drugs in complex food matrices. [13][14][15][16][17] Despite the advantages that the QuEChERS method offers, there are still some disadvantages such as insufficient clean-up and difficulty automating. To reduce labor and improve precision, laboratory automation has been implemented through the utilization of robotic sample preparation tools in sample cleanup.…”