Development and validation of a reversed-phase liquid chromatography method for the quantitative determination of carboxylic acids in industrial reaction mixtures

Destandau, Émilie; Vial, Jérôme; Jardy, Alain; Hennion, M.; Bonnet, D.; Lancelin, Pierre

doi:10.1016/j.chroma.2005.01.002

Cited by 52 publications

(36 citation statements)

References 23 publications

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“…NBD-ceramide was completely recovered in the organic phase, whereas over 95% of NBD-caproic acid was recovered in the aqueous phase (Fig. 1A) as reported previously (29,30). Although NBD-ceramide and NBD-caproic acid showed similar Rf values (about 0.9), the two compounds could be separated because of the different solubility in organic and aqueous solutions.…”

Section: Detection Of Nbd-caproic Acid a Counterpart Of Ceramidase-msupporting

confidence: 83%

Activation of Ceramidase and Ceramide Kinase by Vanadate via a Tyrosine Kinase–Mediated Pathway

Tada¹,

Toyomura²,

Nakamura³

et al. 2010

J Pharmacol Sci

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Abstract. Ceramide, a key molecule in the metabolism of sphingolipids, is converted by ceramidase to sphingosine, and phosphorylated by ceramide kinase to form ceramide-1-phosphate (C1P). In this study, we improved on a method of thin-layer chromatography using a fluorescent ceramide, 4-nitrobenzo-2-oxa-1,3-diazole-labeled C6-ceramide (NBD-ceramide) by adding another step for separation of extracted ceramide metabolites by lipophilicity, and determined levels of C1P, caproic acid, sphingomyelin, and glucosylceramide simultaneously. Also we found that 1) treatment of NBD-ceramide-labeled cells (human lung adenocarcinoma A549 cells and Chinese hamster ovary cells) with Na 3 VO 4 increased the amount of NBD-C1P formed within 30 min, 2) the treatment increased production of NBD-caproic acid, a counterpart of sphingosine, by ceramidase within 2 h, 3) expression of ceramide kinase enhanced the Na 3 VO 4 -induced formation of NBD-C1P, and tyrosine kinase inhibitors (herbimycin and genistein) decreased the response, 4) the production of NBD-caproic acid in A549 cells was inhibited by genistein, and 5) the responses for 2 h after Na 3 VO 4 treatment were accompanied by a decrease in the production of NBD-sphingomyelin, not a loss of NBD-ceramide. The improved thin-layer chromatography method was useful for the simultaneous determination of enzymatic activities for ceramide metabolism in cells.

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Section: Detection Of Nbd-caproic Acid a Counterpart Of Ceramidase-msupporting

confidence: 83%

Activation of Ceramidase and Ceramide Kinase by Vanadate via a Tyrosine Kinase–Mediated Pathway

Tada¹,

Toyomura²,

Nakamura³

et al. 2010

J Pharmacol Sci

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“…It may be detected on LC-MS instruments with atmospheric pressure ionization in negative mode (21 ). Physiological concentrations of MMA in serum/plasma are very low, however, and the structural isomer of MMA, SA, is often indistinguishable from MMA (14 ) with reversed-phase columns (22 ). Although some investigators have recently succeeded in separating MMA on octadecyl silane columns (13 ), chromatographic selectivity and detection remain the main challenges to be overcome during method development.…”

Section: Discussionmentioning

confidence: 99%

Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection

Lakso

Appelblad²,

Schneede

2008

Clinical Chemistry

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“…Microextração em fase sólida [44][45][46] Um procedimento ainda pouco utilizado, mas muito eficaz, na preparação de amostras de compostos carbonilados e carboxilados é a microextração em fase sólida (SPME). A técnica baseia-se no princípio da sorção sobre fibra seletiva ou sobre fibra que possibilite a derivatização em sua superfície.…”

Section: Extração Gás-líquido Ou Líquido-líquidounclassified

Determinação de compostos carbonilados e carboxilados em derivados de petróleo

Vieira¹,

Rebouças²,

Andrade³

2012

Quím. Nova

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Recebido em 17/9/11; aceito em 13/3/12; publicado na web em 10/7/12 CARBONYL AND CARBOXYL COMPOUNDS DETERMINATION IN PETROLEUM PRODUCTS. Although metals and nitrogen/sulfur compounds have been the main concern of the petroleum industry, issues concerning the harmful effects on catalysts poisoning and product contamination by other contaminants such as oxygen-containing compounds have been raised. Trace amounts of carbonyl and carboxyl compounds in petroleum products can lead to catalyst poisoning. Additionally, oxygenates may be present in final polyethylene and polypropylene resins, affecting the quality of food packaging. In this work, we reviewed potential analytical approaches for oxygenates determination in petroleum products and report the features of each potential technique.Keywords: petroleum products; carbonyl; carboxyl. INTRODUÇÃODurante muitos anos, o enxofre foi considerado o principal contaminante dos processos de refino do petróleo e seus derivados. No entanto, atualmente é crescente a preocupação da indústria quanto ao efeito nocivo de outros contaminantes como os compostos oxigenados. Traços de compostos carbonílicos e carboxílicos voláteis presentes no petróleo, suas frações, e especialmente em derivados da primeira geração da indústria petroquímica, tais como eteno e propeno que são utilizados como precursores para produção de resinas termoplásticas (polietileno e polipropileno), podem afetar a qualidade das embalagens obtidas a partir desses materiais, comprometendo o sabor dos alimentos.1-5 Além disso, a presença de tais contaminantes provoca a diminuição da atividade e envenenamento não permanente em catalisadores da primeira geração e permanente da segunda geração da indústria petroquímica.Compostos carbonílicos e carboxílicos, tais como aldeídos, cetonas e ácidos carboxílicos de baixa massa molecular (C2-C5) podem advir de contaminações do petróleo ou etanol, matérias-primas das indústrias petroquímicas e alcoolquímica, respectivamente. Além disso, podem resultar de processos de extrusão a altas temperaturas utilizados para produção de polímeros, como polietileno (PE), polipropileno (PP) e policloreto de vinilo (PVC), 3-5 ou, mesmo, estarem associados a reações fotoquímicas de hidrocarbonetos, como é frequentemente reportado em avaliações ambientais do ar de atmosferas urbanas. [6][7][8] De acordo com a literatura atual, há uma quantidade reduzida de métodos analíticos capazes de quantificar os compostos carbonílicos e carboxílicos em matrizes complexas como petróleo e seus derivados com rapidez, precisão e exatidão necessárias. Convém notar, no entanto, que métodos desenvolvidos nas refinarias e indústrias petroquímicas raramente são publicados, permanecendo apenas como documentos de circulação interna nessas empresas. Os métodos desenvolvidos para essas aplicações buscam conciliar necessidades diversas e eventualmente conflitantes, como elevada sensibilidade, separação eficiente para eliminação da matriz, minimização das perdas do analito por adsorção na superfície interna de injetores ...

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Development and validation of a reversed-phase liquid chromatography method for the quantitative determination of carboxylic acids in industrial reaction mixtures

Cited by 52 publications

References 23 publications

Activation of Ceramidase and Ceramide Kinase by Vanadate via a Tyrosine Kinase–Mediated Pathway

Activation of Ceramidase and Ceramide Kinase by Vanadate via a Tyrosine Kinase–Mediated Pathway

Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection

Determinação de compostos carbonilados e carboxilados em derivados de petróleo

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