Development and validation of a liquid chromatography–tandem mass spectroscopy method for simultaneous determination of (+)-(13aS)-deoxytylophorinine and its pharmacologically active 3-O-desmethyl metabolite in rat plasma

Lu³

et al. 2023

Anal. Chem.

To figure out the reason for the drawback of the stored waveform inverse Fourier transform (SWIFT) waveform and realize the high-resolution ion isolation on the miniaturized linear ion trap mass spectrometer, we studied the efficiency that ions can be excited under different excitation durations and amplitudes at different frequencies and compared the overlap ratios of the effective excitation frequency bandwidths of the adjacent ions. According to this, we proposed a new coarse-to-fine isolation waveform named SWIFTSIN. By superposing one or more sinusoidal waveforms on the SWIFT waveform and modulating the phases of the superposed sinusoidal waveforms, the generated SWIFTSIN waveform can achieve unit mass isolation on the miniaturized linear ion trap mass spectrometer without reducing the intensity of the target ion. The isolation ability of the SWIFTSIN waveform was verified by isolating a single isotope peak in the mixed samples.

Section: Resultsmentioning

confidence: 98%

SWIFTSIN: A High-Resolution Ion Isolation Waveform for the Miniaturized Linear Ion Trap Mass Spectrometer by Coarse to Fine Excitation

Lu³

et al. 2023

Anal. Chem.

“…5d plots the secondary mass spectrum, where the isotope ion at m/z 135 corresponded to the fragment ion of rosiglitazone, and the isotope ions at m/z 165 and m/z 192 corresponded to the fragment ions of rotundine. [40][41][42][43] In addition, the isotope ion at m/z 136 also appeared (inside Figure 5e), according to the fragmentation law, which can be considered that the ion was the fragmentation of the rosiglitazone isotope. The D isotope ion obtained after fine isolation was fragmented, as in the interior of Figure 5e, only the fragment peak was retained at m/z 135 and other fragment peaks disappeared.…”

Section: Resultsmentioning

confidence: 99%

SWIFTSIN: A High-Resolution Ion Isolation Waveform for the Miniaturized Linear ion Trap Mass Spectrometer by Coarse to Fine Excitation

et al. 2022

Preprint

To figure out the reason for the drawback of the SWIFT waveform and realize the high-resolution ion isolation on the miniaturized linear ion trap mass spectrometer, we studied the efficiency that ions can be excited under different excitation periods and amplitudes at different frequencies and compared the overlap rates of the effective excitation bandwidths of the adjacent ions. According to this, we purposed a new coarse-to-fine isolation waveform named SWIFTSIN. By superposing one or more sinusoidal waveforms on the SWIFT waveform and modulating the phases of the superposed sinusoidal waveforms, the generated SWIFTSIN waveform achieved unit mass isolation on the miniaturized linear ion trap mass spectrometer without reducing the intensity of the target ion. The isolation ability of the SWIFTSIN waveform was verified by isolating a single isotope peak in the mixed samples.

“…Figure 5d plots the MS/MS spectrum, where the isotope ion at m/z 135 corresponded to the fragment ion of rosiglitazone, and the isotope ions at m/z 165 and m/z 192 corresponded to the fragment ions of rotundine. [39][40][41][42] In addition, the isotope ion at m/z 136 also appeared (blue line of the inset of Figure 5c), according to the fragmentation law, which can be considered that the ion was the fragmentation of the rosiglitazone isotope D+1. On the other hand, when the \isotope ion D obtained after fine isolation was fragmented, as indicated by the red dashed line of the inset of Figure 5c, the fragment peak was only retained at m/z 135 and other fragment peaks disappeared.…”

Section: Resultsmentioning

confidence: 99%

SWIFTSIN: A High-Resolution Ion Isolation Waveform for the Miniaturized Linear ion Trap Mass Spectrometer by Coarse to Fine Excitation

et al. 2022

Preprint

To figure out the reason for the drawback of the SWIFT waveform and realize the high-resolution ion isolation on the miniaturized linear ion trap mass spectrometer, we studied the efficiency that ions can be excited under different excitation durations and amplitudes at different frequencies and compared the overlap ratios of the effective excitation frequency bandwidths of the adjacent ions. According to this, we proposed a new coarse-to-fine isolation waveform named SWIFTSIN. By superposing one or more sinusoidal waveforms on the SWIFT waveform and modulating the phases of the superposed sinusoidal waveforms, the generated SWIFTSIN waveform can achieve unit mass isolation on the miniaturized linear ion trap mass spectrometer without reducing the intensity of the target ion. The isolation ability of the SWIFTSIN waveform was verified by isolating a single isotope peak in the mixed samples.