Development and validation of a near infrared spectrophotometric method to determine total antioxidant activity of milk

Niero, Giovanni; Penasa, M.; Currò, Sarah; Masi, Antonio; Trentin, Anna Rita; Cassandro, Martino; Marchi, Massimo De

doi:10.1016/j.foodchem.2016.10.024

Cited by 23 publications

(21 citation statements)

References 27 publications

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“…However, FMPO 150 was IC 50 (67.60 ± 1.04 μg/ml) compared to FM which was IC 50 (120.41 ± 7.74 μg/ml) at the same point during storage. In general, these antioxidant activity values of fermented milk are greater than whole milk, as reported by Niero et al [38], due to the addition of pomegranate peel extract in fermented milk. Mousavi et al [39] also reported that fermentation by L. plantarum and L. acidophilus reduced the concentration of phenolic compounds and increased the antioxidant activity of pomegranate juice because some LAB strains can degrade certain phenolic compounds into other metabolites with higher antioxidant activities [40].…”

Section: Antioxidant Activity Of the Functional Milk Beveragessupporting

confidence: 52%

Production of Functional Fermented Milk Beverages Supplemented with Pomegranate Peel Extract and Probiotic Lactic Acid Bacteria

Al-Hindi

Ghani

2020

Journal of Food Quality

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Fermented milk beverages supplemented with pomegranate peel extract and inoculated with Lactobacillus plantarum and Bifidobacterium longum subsp longum were produced. The antioxidant activity of fermented milk beverages supplemented with pomegranate peel 150 mg/L (FMPO 150) and 300 mg/L (FMPO 300) was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). In addition, the polyphenolic profile and sugars content were determined by HPLC analysis, and the volatile compounds were identified using GC-MS analysis. The effects of FMPO 150 and FMPO 300 (10 g/day) on the lipid profile and antioxidant/biochemical status of rats were also evaluated after 4 weeks of oral intake. Antioxidant activity of the fermented milk beverage FMPO 300 was higher than that of FMPO 150. GC-MS analysis of the volatile compounds revealed that diacetyl, acetoin, and acetaldehyde were the major constituents. FMPO 150 and FMPO 300 were efficient in reducing the LDL cholesterol and triacylglycerol and increased the HDL cholesterol in serum. Liver function biomarkers were not affected by the end of treatment (p<0.05). Also, the thiobarbituric acid-reactive substances (TBARS) were decreased, while the activity of antioxidant enzymes in the liver (GSH, CAT, SOD, and GPx) were increased. Hence, the combination of pomegranate peel extract and probiotic lactic acid bacteria in a fermented milk beverage provides not only probiotic benefits but also bioactive phenolic compounds that could be functional and possess therapeutic effects.

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Section: Antioxidant Activity Of the Functional Milk Beveragessupporting

confidence: 52%

Production of Functional Fermented Milk Beverages Supplemented with Pomegranate Peel Extract and Probiotic Lactic Acid Bacteria

Al-Hindi

Ghani

2020

Journal of Food Quality

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“…6,12,15 Organic solvents, such as ethanol, acetone and acetonitrile, have also been reported as protein precipitating agents in milk and other samples. 14,[16][17][18][19] Several analytical methods have been reported for the determination of vitamin C, e.g., enzymatic, 20 spectrophotometric 21 and separation techniques such as high-performance liquid chromatography (HPLC), which provide more accurate results. HPLC has been used extensively for L-AA determination in various foods, 7,8,[22][23][24][25] including milk.…”

Section: Introductionmentioning

confidence: 99%

Determination of L-Ascorbic Acid in Milk by Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry Analysis

Zappielo¹,

Nicácio²,

Manin³

et al. 2019

J. Braz. Chem. Soc.

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This study provides a fast, accurate and reproducible method for L-ascorbic acid (L-AA) determination in milk samples by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). A small volume of a low toxicity organic solvent (ethanol) was used for degreasing and deproteinization steps. Ethylenediaminetetraacetic acid disodium salt (EDTA) and formic acid were used as stabilizing agents. The method was successfully validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and inter/intra-day precision and applied in raw and processed milk samples. Good linearity (r 2 > 0.9915) and low LOD and LOQ, 1.5 and 5.0 µg L-1 , respectively, were obtained. The recoveries for 500 and 1000 µg L-1 spikes were higher than 90% and the precision values expressed in terms of relative standard deviation (RSD) were ≤ 6.8%. For the first time, the determination of L-AA in a 500 µg L-1 concentration range was reported for milk samples.

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“…Repeatability was expressed as relative standard deviation (RSD r ) of 5 aliquots from the same bulk milk, processed separately. Reproducibility was calculated as relative standard deviation (RSD R ) of 15 samples measured across 3 d (Niero et al, 2017).…”

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confidence: 99%

Technical note: Development and validation of a new method for the quantification of soluble and micellar calcium, magnesium, and potassium in milk

Franzoi

Niero

Penasa

et al. 2018

Journal of Dairy Science

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Milk mineral content is a key trait for its role in dairy processes such as cheese-making, its use as source of minerals for newborns, and for all traits involving salt-protein interactions. This study investigated a new method for measuring mineral partition between soluble and micellar fractions in bovine milk after rennet coagulation. A new whey dilution step was added to correct the quantification bias due to whey trapped in curd and excluded volume. Moreover, the proposed method allowed the quantification of the diffusible volume after milk coagulation. Milk mineral content and concentration in whey, and diluted whey were quantified by acid digestion and inductively coupled plasma optical emission spectrometry. The repeatability of the method for micellar Ca, Mg, and K was between 2.07 and 8.96%, whereas reproducibility ranged from 4.01 to 9.44%. Recovery of total milk minerals over 3 spiking levels ranged from 92 to 97%. The proposed method provided an accurate estimation of micellar and soluble minerals in milk, and curd diffusible volume.

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Development and validation of a near infrared spectrophotometric method to determine total antioxidant activity of milk

Cited by 23 publications

References 27 publications

Production of Functional Fermented Milk Beverages Supplemented with Pomegranate Peel Extract and Probiotic Lactic Acid Bacteria

Production of Functional Fermented Milk Beverages Supplemented with Pomegranate Peel Extract and Probiotic Lactic Acid Bacteria

Determination of L-Ascorbic Acid in Milk by Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry Analysis

Technical note: Development and validation of a new method for the quantification of soluble and micellar calcium, magnesium, and potassium in milk

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