Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis

Gaona-Galdos, Angel Arturo; Filho, Luiz Antonio Zanolli; Tavares, Marina Franco Maggi; Aurora-Prado, Maria Segunda; Santoro, María Inés Rocha Miritello; Kedor-Hackmann, Érika Rosa Maria

doi:10.1016/j.chroma.2008.03.070

Cited by 13 publications

(6 citation statements)

References 30 publications

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“…In addition, an increase in the concentration of SDS caused a significant increase in the migration time of econazole enantiomers due to the increasing viscosity of the solution at increasing SDS concentration. This observation has also been reported by other authors [8,17,18]. In light of these aspects, optimal concentration chosen for SDS was 50 mM.…”

Section: Resultssupporting

confidence: 67%

“…Quantitative determination of econazole nitrate (pK a = 6.6) in cream formulation by CZE using 20 mM phosphate buffer adjusted to pH 2.5 with phosphoric acid has also been reported with an analysis time of 1.2 min. However, no chiral separation was performed [18]. Quantitative determination of econazole in pessaries using 75 mM acetic acid-acetate buffer in methanol:water (20:80, v/v) (pH 5.18) was also performed and no enantiomeric separation was achieved [19].…”

Section: Introductionmentioning

confidence: 99%

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Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-γ-cyclodextrin

Hermawan

Ibrahim

Sanagi

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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Section: Resultssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-γ-cyclodextrin

Hermawan

Ibrahim

Sanagi

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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“…The RSDs of the peak area ratios (SL/IS and CN/IS) were 1.3, 1.6% (intraday), and 2.9, 3.7% (interday), respectively. To determine the method accuracy, recovery experiments were performed according standard addition procedures [23]. Accuracy was calculated as the percentage recovery of a known amount of SL and CN standards added to the samples.…”

Section: Analytical Calibrationmentioning

confidence: 99%

Simultaneous separation and rapid determination of spironolactone and its metabolite canrenone in different pharmaceutical formulations and urinary matrices by capillary zone electrophoresis

Huang

Zhao

et al. 2016

J. Sep. Science

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The aim of this study was to develop a novel, sensitive, precise, simple, and rapid capillary zone electrophoresis method for the quality control of spironolactone in three different formulation types and a rapid simultaneous determination of the content of spironolactone and canrenone in urine samples using fluocinonide as an internal standard. After optimization of separation conditions, the electrolyte solution was the pH 5.5, 20 mM phosphate buffer containing 4.5 g/L sulfated-β-cyclodextrin, 15 kV of electric filed across the capillary applied at 25°C. A diode array detector was used, and the detection wavelength was 260 nm. Under optimum conditions, good linearity was achieved with correlation coefficients from 0.9976 to 0.9997. Detection limits were 0.56 and 0.20 μg/mL, and the quantitation limits were 1.87 and 0.67 μg/mL, respectively. Excellent accuracy and precision were obtained. Recoveries of the analytes varied from 100.8 to 103.1%. The results indicated that baseline separation of analytes was obtained and this method was suitable for quantitative determination of spironolactone in pharmaceutical preparations and rapid simultaneous determination of the content of spironolactone and its major metabolite canrenone in urine samples.

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“…are suggested by the Food and Drug Administration (FDA) as therapeutic options for fungal infections. Among those, azole antifungal agents are the most rapidly expanding group [2,3].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

2011

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Hollow fiber liquid-phase microextraction (HF-LPME) based on two immiscible organic solvents (n-dodecane as membrane solvent and 22 lL acetonitrile as acceptor phase) was evaluated for extraction and preconcentration of trace amounts of miconazole and clotrimazole as model azole antifungal drugs, prior to determination by gas chromatography (GC). The drugs were extracted from 24 mL sample solution into a thin layer of organic phase (n-dodecane) immobilized in hollow fiber pores, and then back-extracted into approximately 22 lL organic phase (acetonitrile) located in the lumen of the hollow fiber. After extraction, 1 lL acceptor phase was injected into a GC instrument. Under optimum conditions, limits of detection (LODs) ranged from 0.3-6.0 ng mL -1 , linear dynamic range of 1-400 ng mL -1 and relative standard deviations (RSD%) \5% were obtained. The developed method provided preconcentration factors of 639 and 496 for clotrimazole and miconazole, respectively. The applicability of the extraction method to different types of complicated matrices such as milk, plasma and urine samples was investigated. The results obtained results reveal that the proposed method is a good technique for extraction and determination of the target drugs in different matrices.

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Development and validation of a method for quantitative determination of econazole nitrate in cream formulation by capillary zone electrophoresis

Cited by 13 publications

References 30 publications

Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-γ-cyclodextrin

Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-γ-cyclodextrin

Simultaneous separation and rapid determination of spironolactone and its metabolite canrenone in different pharmaceutical formulations and urinary matrices by capillary zone electrophoresis

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

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