Development and Validation of a New HPLC-UV Method for the Simultaneous Determination of Triclabendazole and Ivermectin B1a in a Pharmaceutical Formulation

Shurbaji, Maher; Rub, Mohamad H Abu Al; Saket, M.; Qaisi, Ali M.; Salim, Maher L.; Abu-Nameh, Eyad S. M.

doi:10.1093/jaoac/93.6.1868

Cited by 25 publications

(12 citation statements)

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“…Samples were diluted in methanol and analyzed by High Performance Liquid Chromatography (HPLC) against standards according to a method approved by the Association of Official Agricultural Chemist. 9 selection of farms. Two farms (Tarso and San Jeronimo) where producers experienced treatment failure after using one or several products containing ivermectin were visited on separate occasions within days of dosing to substantiate further investigation.…”

Section: Methodsmentioning

confidence: 99%

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Reduced Efficacy of Commercial Acaricides against Populations of Resistant Cattle Tick Rhipicephalus microplus from Two Municipalities of Antioquia, Colombia

Lopez-Arias

Villar-Argaiz

Chaparro-Gutiérrez

et al. 2014

Environ�Health�Insights

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Two distant Antioquian cattle farms where systemic and topical acaricides had previously failed to control infestations by Rhipicephalus (Boophilus) microplus were studied. An initial in vivo study was conducted using single subcutaneous injections with a long-acting formulation of ivermectin (630 μg/kg). Injections were made at 3-month intervals on animals at each farm to evaluate the therapeutic and persistent efficacy of ivermectin against R. microplus. Body tick counts and reproductive parameters of semi- or fully engorged females (≥5 mm) were assessed at 10-day intervals, and since no negative control group could be included, values were compared against those for day 0. Although there was an overall reduction of 50%–75% in tick numbers that persisted for 30–40 days, it was not significantly different at one of the farms and not enough to afford protection from severe infestations. The engorgement weight and egg mass weight of ticks from treated animals were significantly lower throughout the 50-day posttreatment period. Egg hatch was not significantly reduced posttreatment and remained at levels of 80%–90%. A random selection of 9 out of 28 commercial formulations of ivermectin sold in Colombia were analyzed by High Performance Liquid Chromatography (HPLC). All were within the expected labeled concentration (±15% deviation) of 1% and 3.15% ivermectin except for one. A popular unregistered injectable widely used in both farms and labeled as “natural pyrethrin”, was found to contain 10.5% ivermectin. An adult immersion test was conducted to evaluate the efficacy of topical acaricides to recommended concentrations of five commercial products and/or their combinations. Efficacy was determined by comparing the reproductive index of each treated group to that of the control group. Cypermethrin (150 ppm) was completely ineffective at both farms. Amitraz (208 ppm) exhibited low and intermediate efficacies of 14% and 56%. The combination of amitraz (100 ppm) and cypermethrin (150 ppm) was less efficacious than the amitraz alone. A generic product based on amitraz + citronella (208 ppm + 10 ppm, respectively) was shown to be less efficacious than the name-brand amitraz product. Products containing the organophosphate chlorpyrifos or trichlorfon exhibited intermediate efficacies of approximately 60% at the Tarso farm. We conclude that at these two locations, there is a high degree of resistance to many of the acaricides available in Colombia and confirm suspicions that ivermectin is no longer able to eliminate tick infestations.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Reduced Efficacy of Commercial Acaricides against Populations of Resistant Cattle Tick Rhipicephalus microplus from Two Municipalities of Antioquia, Colombia

Lopez-Arias

Villar-Argaiz

Chaparro-Gutiérrez

et al. 2014

Environ�Health�Insights

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“…For these reason, different methods using chromatography have been described for the analysis of IVM. Shurbaji et al [9] developed a precise, accurate, and sensitive chromatography method coupled to ultra violet detector (UV) to identify simultaneously triclabendazole and IVM in a pharmaceutical formulation. However, UV did not provide the specificity required because it showed limitations at detection of low levels of IVM in plasma, tissue and milk [10].…”

Section: Introductionmentioning

confidence: 99%

Development of HPLC-Fluorescence Method for the Determination of Ivermectin Residues in Commercial Milk

Machado¹,

Rezende²

2016

J Exp Food Chem

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Objective: A study aimed at the validation of a high performance liquid chromatography (HPLC) method for detection and quantification of ivermectin (IVM) in commercial milk. Method: After a liquid-liquid extraction with acetonitrile and hexane, a derivatization with a mixture of trifluoroacetic acid and trifluoroacetic anhydride was performed. Chromatography conditions were a C8 column, a fluorescent detector (Ex. 360; Em. 470 nm), a run time of 25 minutes and an isocratic elution at 30°C. The methodology was validated in terms of selectivity, linearity, precision, recovery, limit of quantification (LoQ), limit of detection (LoD), and robustness. Results: The method showed an adequate linearity and selectivity (r 2 =0.999) with an elution time of 13.909 minutes. However, the repeatability and intermediate precision showed RSD values above of 20% and recovery between 37 to 79%. The calculated LoD and LoQ were 2.50 and 5.00 ng/ml respectively. Robustness showed a significant variation on the analytical method with small changes in heating time during the derivatization and flow rate of chromatography system. Conclusion: The HPLC-fluorescence method showed results partially satisfactory and could be the used for IVM detection in commercial milk samples.

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“…In the literature, the determination of PRA in its mixtures with the active compounds was carried out by several methods including spectrophotometry [3], HPLC [4][5][6][7]. In previous studies, ivermectin in samples with other active compounds was analyzed by using chromatography [8][9][10][11][12][13][14]. In the simultaneous analysis of PRA and IVE in commercial formulations, four different methods were reported, including HPLC [15,16], LC-MSMS [17,18].Simultaneous determination of analytes in the same sample requires the separation procedure or pretreatment based on the use of chromatographic methods.…”

Section: Introductionmentioning

confidence: 99%

Application of PCR and PLS Tools for the Simultaneous Quantification of Praziquantel and Ivermectin Binary Mixtures in Veterinary Tablets

BÜKER

Dinç

YAŞAR

2022

Düzce Üniversitesi Bilim Ve Teknoloji Dergisi

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Two chemometric calibration methods, principal component regression (PCR) and partial least squares regression (PLS) were proposed for the simultaneous spectrophotometric determination of praziquantel (PRA) and ivermectin (IVE) in a marketed veterinary formulation without using preliminary separation step. UV spectra of calibration set and samples including PRA and IVE were recorded in the spectral region of 225-315 nm. PCR and PLS algorithms were applied to absorbance data matrix and concentration set including PRA and IVE in the linear concentration range of 20.0-160.0 µg/mL for PRA and 2.0-44.0 µg/mL for IVE. The capability of the PCR and PLS methods were validated by analyzing validation samples. Assay results showed that both PCR and PLS approaches provided an opportunity for quantifying PRA and IVE in veterinary tablet formulation.

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Development and Validation of a New HPLC-UV Method for the Simultaneous Determination of Triclabendazole and Ivermectin B1a in a Pharmaceutical Formulation

Cited by 25 publications

References 0 publications

Reduced Efficacy of Commercial Acaricides against Populations of Resistant Cattle Tick Rhipicephalus microplus from Two Municipalities of Antioquia, Colombia

Reduced Efficacy of Commercial Acaricides against Populations of Resistant Cattle Tick Rhipicephalus microplus from Two Municipalities of Antioquia, Colombia

Development of HPLC-Fluorescence Method for the Determination of Ivermectin Residues in Commercial Milk

Application of PCR and PLS Tools for the Simultaneous Quantification of Praziquantel and Ivermectin Binary Mixtures in Veterinary Tablets

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