Development and validation of a gas chromatography–mass spectrometry method to analyze octanoate enrichments at low concentrations in human plasma

Harskamp, Dewi van; Knottnerus, Suzan J. G.; Visser, Gepke; Goudoever, Johannes B. van; Schierbeek, Henk

doi:10.1007/s00216-020-02801-7

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…1 As a second example, Mercier and colleagues used MS−MS in the design of a method to simultaneously analyze over 40 semivolatile organic compounds of health interest in indoor dust. 2 In fact, the power of second stage mass spectrometry has been harnessed in a variety of applications, ranging from environmental 3 to food 4 to clinical 5 chemistry.…”

Section: ■ Introductionmentioning

confidence: 99%

Qualitative Analysis of Acetophenone, Naphthalene, and Hexadecane by GC-Triple-Quad-MS Using MRM

Slonecker

Vonderheide

2022

J. Chem. Educ.

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The use of mass spectrometry (MS) is becoming widespread and rather routine in all levels of industry, government, and academia. Coupled with chromatographic methods, this powerful instrumental technique can be used to elucidate the composition of a complex sample and definitively identify constituents while offering the benefits of high-precision quantitative analysis and low detection limits. These attributes, as well as its growing employment, emphasize the need for the undergraduate student to gain practical experience with this instrumental technique. Additionally, it is critical that students observe first-hand the abilities and contrasts of MS analysis versus MS−MS analysis or triple-quadrupole (Q 1 q 2 Q 3 ) multiple reaction monitoring (MRM) analysis. The focus of this experiment was the qualitative analysis of a sample containing three compounds: acetophenone, naphthalene, and hexadecane using a Q 1 q 2 Q 3 GC−MS in single-quad full-scan mode, Q 1 q 2 Q 3 in full-scan mode, and Q 1 q 2 Q 3 in MRM mode. The analytes were specifically chosen to range from extensive fragmentation (molecular ion of low stability) to minimal fragmentation (molecular ion of high stability). Students utilized the MS NIST library to identify the unknown peaks after analysis using the first quadrupole in full-scan mode. They further compared different injection volumes and observed the effect of injection volume on the band shapes and the signal to noise ratio. The students then compiled a table of precursor ion MS peaks from the single-quad full-scan mode. The sample was next analyzed using Q 1 q 2 Q 3 in full-scan mode, and students compiled a table of product ion MS peaks. These were translated to transition ion pairs and compared to instructor-chosen transition ion pairs used in the final MRM analysis. This laboratory exercise allowed the students to use modern instrumentation and analytical techniques to investigate the potential of second stage mass spectrometry, increasing their technical ability and understanding of this critical scientific tool. Moreover, they were able to observe first hand the power of the NIST library for unknown analytes as well as the potential advantages of MRM when selectivity and low detection limits are critical.

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Section: ■ Introductionmentioning

confidence: 99%

Qualitative Analysis of Acetophenone, Naphthalene, and Hexadecane by GC-Triple-Quad-MS Using MRM

Slonecker

Vonderheide

2022

J. Chem. Educ.

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Simple, fast and inexpensive quantification of glycolate in the urine of patients with primary hyperoxaluria type 1

et al. 2023

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In primary hyperoxaluria type 1 excessive endogenous production of oxalate and glycolate leads to increased urinary excretion of these metabolites. Although genetic testing is the most definitive and preferred diagnostic method, quantification of these metabolites is important for the diagnosis and evaluation of potential therapeutic interventions. Current metabolite quantification methods use laborious, technically highly complex and expensive liquid, gas or ion chromatography tandem mass spectrometry, which are available only in selected laboratories worldwide. Incubation of ortho-aminobenzaldehyde (oABA) with glyoxylate generated from glycolate using recombinant mouse glycolate oxidase (GO) and glycine leads to the formation of a stable dihydroquinazoline double aromatic ring chromophore with specific peak absorption at 440 nm. The urinary limit of detection and estimated limit of quantification derived from eight standard curves were 14.3 and 28.7 µmol glycolate per mmol creatinine, respectively. High concentrations of oxalate, lactate and L-glycerate do not interfere in this assay format. The correlation coefficient between the absorption and an ion chromatography tandem mass spectrometry method is 93% with a p value < 0.00001. The Bland–Altmann plot indicates acceptable agreement between the two methods. The glycolate quantification method using conversion of glycolate via recombinant mouse GO and fusion of oABA and glycine with glyoxylate is fast, simple, robust and inexpensive. Furthermore this method might be readily implemented into routine clinical diagnostic laboratories for glycolate measurements in primary hyperoxaluria type 1.

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Development and validation of a gas chromatography–mass spectrometry method to analyze octanoate enrichments at low concentrations in human plasma

Cited by 2 publications

References 19 publications

Qualitative Analysis of Acetophenone, Naphthalene, and Hexadecane by GC-Triple-Quad-MS Using MRM

Qualitative Analysis of Acetophenone, Naphthalene, and Hexadecane by GC-Triple-Quad-MS Using MRM

Simple, fast and inexpensive quantification of glycolate in the urine of patients with primary hyperoxaluria type 1

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