Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin

Novel contribution to the simultaneous analysis of certain hypoglycemic drugs in the presence of their impurities and degradation products utilizing UPLC-MS/MS

“…Upon utilization of sufficient collision activated dissociation gas and collision energy, the following MS/MS transitions were carefully chosen 408.12 / 235. 24 IMP1 and IMP2, respectively, as these represented the most abundant products ions (Fig. 1).…”

Physicochemical Properties and Methods of Analysis of Vildagliptin (Review)

“…Studies [ 29 – 35 ] used analytical wavelengths of 239, 260, and 290 nm.We obtained UV spectra in buffer solutions with different pH at concentrations of 10 and 200 μg/ml (Fig. 8 ).…”

“…The dominant technique is reverse-phase HPLC, though, depending on the task undertaken and the concomitant components, ion-pair [ 7 , 14 , 39 , 47 ] chromatography is also found. Cyano-phase separation is reported in [ 35 , 48 ]. Use of the micellar component of sodium dodecylsulfate in the mobile phase is described in [ 51 ].…”

New Analytical Methods for the Determination of Two Gliptin Drugs In Pharmaceutical Formulations and Urine Samples

“…[15][16][17][18][19][20] Literature survey represented that saxagliptin can be determined by several methods including; spectrophotometry [17][18][19][20] , TLC [21][22][23] and HPLC methods. [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42] Many of these methods involve several time-consuming manipulations, extraction steps, derivatization reactions that are liable to various interferences, and are not applicable to colored and turbid solutions.…”