Development and utilization of new O2-independent bioreporters
Eva Agranier,
Pauline Crétin,
Aurélie Joublin-Delavat
et al.
Abstract:Fluorescent proteins have revolutionized science since their discovery in 1962. They have enabled imaging experiments to decipher the function of proteins, cells and organisms, as well as gene regulation. GFP and all its derivatives are now standard tools in cell biology, immunology, molecular biology and microbiology laboratories around the world. A common feature of these proteins is their O2-dependent maturation allowing fluorescence, which precludes their use in anoxic contexts. In this work, we report the… Show more
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