Development and utilization of an efficient cytoplasmic male sterile system for Cai-xin (Brassica rapa L.)

Peng, Ying; Shi, Dongquan; Zhang, Tian; Li, Xuehong; Fu, Tingdong; Xu, Yuejin; Wan, Zhengjie

doi:10.1016/j.scienta.2015.04.002

Cited by 13 publications

(12 citation statements)

References 21 publications

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“…, Peng et al. ,b); yet, this is the first time it has been used for hybridization in Nicotiana . Our results show that it is convenient and effective in hybrid production in these crosses.…”

Section: Discussionmentioning

confidence: 99%

“…However, viable hybrid seeds could only be obtained when using the cytoplasmic male sterile line as the female parent, which was similar to the results reported by Laskowska and Berbec (2005). It has been proved that CMS lines are superior in improving hybrid yield and quality in many crops such as Maize and Brassica rapa L (Urs et al 2004, Peng et al 2015a; yet, this is the first time it has been used for hybridization in Nicotiana. Our results show that it is convenient and effective in hybrid production in these crosses.…”

Section: Discussionmentioning

confidence: 99%

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Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

et al. 2017

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In order to introduce the Tomato Spotted Wilt Virus (TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility (CMS) line of N. tabacum (N. tabacum L. cv. (gla.) S ‘K326’), was successfully crossed with N. alata. Despite a high DNA content variability, F1 hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F1 plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F1 subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N. tabacum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

et al. 2017

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“…It is possible that non‐cleavage of the bicistronic ( orf108‐atp1 ) transcript takes place in the pollenless B. oleracea De ‐CMS lines as well as in the B. juncea De ‐CMS lines. Indeed, alloplasmic B. rapa lines carrying D. erucoides cytoplasm showed that anther differentiation appeared at the early to middle mononucleate microspore stage (Peng et al., ); this phenotype differed from that of alloplasmic B. oleracea D e‐CMS line and alloplasmic B. juncea De ‐CMS line. The molecular mechanism of De ‐CMS in both B. oleracea and B. rapa is still being elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the introduction of the alien cytoplasm from D. erucoides seems to be more effective in improving plant growth than the gaps indicated in Figure b. In addition to the B. oleracea De ‐CMS lines, alloplasmic B. rapa line and B. juncea line carrying D. erucoides cytoplasm also showed improvements of growth in plant size and seed size, respectively (Malik et al., ; Peng et al., ). Similarly, variations in phenotype caused by the interactions between the nuclear and organellar genomes have also been observed in other plant species, such as wheat (Soltani et al., ), maize (Allen, ), rice (Wang, Tang, & Tang, ) and Arabidopsis (Moison et al., ; Roux et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Diplotaxis erucoides used as a cytoplasmic resource in this study is phylogenetically close to Brassica cultivated species (Lysak, Koch, Pecinka, & Schubert, ; Pradhan, Prakash, Mukhopadhyay, & Pental, ; Quiros, Ochoa, & Douches, & Vyas, Prakash, & Shivanna, ). D. erucoides cytoplasm conferred CMS in B. juncea (Bhat, Vijayan, Dwivedi, & Prakash, ) and B. rapa (Peng et al., ; Wan et al., ). We have ever tackled to produce B. oleracea line carrying D. erucoides cytoplasm by means of intergeneric crossing between D. erucoides and B. oleracea , and successive backcrossing with B. oleracea .…”

Section: Introductionmentioning

confidence: 99%

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Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant

et al. 2018

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In Brassica oleracea, production of F1 hybrid seeds mainly makes use of the improved Ogura cytoplasmic male sterile (CMS) line. However, reliance on one particular line is a risk, and it would be advantageous to develop other CMS lines. In this study, we transferred Diplotaxis erucoides cytoplasm to B. oleracea cultivars using an alloplasmic B. rapaCMS line as a bridge plant to avoid incompatibility between donor and recipient plants. The new B. oleraceaCMS lines, which were derived by four generations of backcrossing, had small rudimentary anthers with no pollen grain and showed complete male sterility. There was no functional defect in other floral organs, and the ability to receive normal pollen did not appear to be impaired. Moreover, the B. oleraceaCMS lines carrying D. erucoides cytoplasm had larger leaf areas and a normal plastochron. As a consequence, the B. oleraceaCMS lines carrying D. erucoides cytoplasm have the potential to be valuable alternatives for use in commercial B. oleracea hybrid seed production.

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Using wild relatives and related species to build climate resilience in Brassica crops

et al. 2021

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Climate change will have major impacts on crop production: not just increasing drought and heat stress, but also increasing insect and disease loads and the chance of extreme weather events and further adverse conditions. Often, wild relatives show increased tolerances to biotic and abiotic stresses, due to reduced stringency of selection for yield and yield-related traits under optimum conditions. One possible strategy to improve resilience in our modern-day crop cultivars is to utilize wild relative germplasm in breeding, and attempt to introgress genetic factors contributing to greater environmental tolerances from these wild relatives into elite crop types. However, this approach can be difficult, as it relies on factors such as ease of hybridization and genetic distance between the source and target, crossover frequencies and distributions in the hybrid, and ability to select for desirable introgressions while minimizing linkage drag. In this review, we outline the possible effects that climate change may have on crop production, introduce the Brassica crop species and their wild relatives, and provide an index of useful traits that are known to be present in each of these species that may be exploitable through interspecific hybridization-based approaches. Subsequently, we outline how introgression breeding works, what factors affect the success of this approach, and how this approach can be optimized so as to increase the chance of recovering the desired introgression lines. Our review provides a working guide to the use of wild relatives and related crop germplasm to improve biotic and abiotic resistances in Brassica crop species.

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Development and utilization of an efficient cytoplasmic male sterile system for Cai-xin (Brassica rapa L.)

Cited by 13 publications

References 21 publications

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

Production of a desirable Brassica oleraceaCMS line using an alloplasmic B. rapaCMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant

Using wild relatives and related species to build climate resilience in Brassica crops

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