Development and Utilization of a Fluorescence-Based Receptor-Binding Assay for the Site 5 Voltage-Sensitive Sodium Channel Ligands Brevetoxin and Ciguatoxin

McCall, Jennifer R.; Jacocks, Henry M.; Niven, Susan C; Poli, Mark; Baden, Daniel G.; Bourdelais, Andrea J.

doi:10.5740/jaoacint.sgemccall

Cited by 37 publications

(20 citation statements)

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“…Alternative methods for CTX detection in fish include receptor binding assays [28,117,125,161,162,163,164,165], various LC-MS methods [3,9,160,162,166,167,168,169,170,171], and other less frequently employed assay systems (see the review by Lewis, 2004 [172]). Folk methods for detecting CTXs in fish may have potential utility [173], but studies are needed to establish the validity of these approaches.…”

Section: Ctx Detection In Fishmentioning

confidence: 99%

An Updated Review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management

et al. 2017

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Ciguatera Fish Poisoning (CFP) is the most frequently reported seafood-toxin illness in the world. It causes substantial human health, social, and economic impacts. The illness produces a complex array of gastrointestinal, neurological and neuropsychological, and cardiovascular symptoms, which may last days, weeks, or months. This paper is a general review of CFP including the human health effects of exposure to ciguatoxins (CTXs), diagnosis, human pathophysiology of CFP, treatment, detection of CTXs in fish, epidemiology of the illness, global dimensions, prevention, future directions, and recommendations for clinicians and patients. It updates and expands upon the previous review of CFP published by Friedman et al. (2008) and addresses new insights and relevant emerging global themes such as climate and environmental change, international market issues, and socioeconomic impacts of CFP. It also provides a proposed universal case definition for CFP designed to account for the variability in symptom presentation across different geographic regions. Information that is important but unchanged since the previous review has been reiterated. This article is intended for a broad audience, including resource and fishery managers, commercial and recreational fishers, public health officials, medical professionals, and other interested parties.

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Section: Ctx Detection In Fishmentioning

confidence: 99%

An Updated Review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management

et al. 2017

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“…Primary stocks of lyophilized Karenia brevis BODIPY conjugated metabolites BODIPY‐PbTx‐2 (B‐PbTx), MW = 1,182.61 g · mol −1 ; BODIPY‐Brevenal (B‐Bn), MW = 944.56 g · mol −1 ; and BODIPY‐Brevisin (B‐Bs) MW = 994.56 g · mol −1 were prepared at the UNCW Center for Marine Sciences (McCall et al. , ) and each made up to a final stock concentration of 1 mM in dimethyl sulfoxide (DMSO). Unconjugated BODIPY (Life Technologies, Carlsbad, CA, USA; MW = 306.12 g · mol −1 ) was a negative control.…”

Section: Methodsmentioning

confidence: 99%

Uptake and localization of fluorescently‐labeled Karenia brevis metabolites in non‐toxic marine microbial taxa

Kramer

Bourdelais

Kitchen

et al. 2018

Journal of Phycology

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Brevetoxin (PbTx) is a neurotoxic secondary metabolite of the dinoflagellate Karenia brevis. We used a novel, fluorescent BODIPY-labeled conjugate of brevetoxin congener PbTx-2 (B-PbTx) to track absorption of the metabolite into a variety of marine microbes. The labeled toxin was taken up and brightly fluoresced in lipid-rich regions of several marine microbes including diatoms and coccolithophores. The microzooplankton (20-200 lm) tintinnid ciliate Favella sp. and the rotifer Brachionus rotundiformis also took up B-PbTx. Uptake and intracellular fluorescence of B-PbTx was weak or undetectable in phytoplankton species representative of dinoflagellates, cryptophytes, and cyanobacteria over the same (4 h) time course. The cellular fate of two additional BODIPY-conjugated K. brevis associated secondary metabolites, brevenal (B-Bn) and brevisin (B-Bs), were examined in all the species tested. All taxa exhibited minimal or undetectable fluorescence when exposed to the former conjugate, while most brightly fluoresced when treated with the latter. This is the first study to observe the uptake of fluorescently-tagged brevetoxin conjugates in non-toxic phytoplankton and zooplankton taxa, demonstrating their potential in investigating whether marine microbes can serve as a significant biological sink for algal toxins. The highly variable uptake of B-PbTx observed among taxa suggests some may play a more significant role than others in vectoring lipophilic toxins in the marine environment.

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“…However, its use of radiolabeled compounds is a major disadvantage, requiring specially trained operators and a license for radiation work. Recently, a binding assay with synaptosomes was developed using a fluorescent ligand, avoiding the use of radiolabeled ligands 45) . The receptor binding assay responds to all sodium channel receptor binding toxins and thus lacks a high level of specificity.…”

Section: -3-3 Receptor Binding Assaymentioning

confidence: 99%

Role of Biomarkers in Monitoring Brevetoxins in <i>Karenia brevis</i> Exposed Shellfish

2018

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Monitoring and management programs for marine toxins in seafood depend on efficient detection tools for their success in protecting public health. Here we review current methods of detection for neurotoxic shellfish poisoning (NSP) toxins, and current knowledge in brevetoxin metabolism in shellfish. In addition, we discuss a novel approach to developing monitoring tools for NSP toxins in molluscan shellfish. NSP is a seafood-borne disease caused by the consumption of brevetoxin-contaminated shellfish. Brevetoxins are a suite of cyclic polyether compounds found in blooms of the marine dinoflagellate Karenia brevis (K. brevis) and are potent neurotoxins. Preventive controls for NSP in the U.S. currently rely upon environmental monitoring of K. brevis blooms and assessment of their shellfish toxicity by mouse bioassay. The mouse bioassay for NSP approved by National Shellfish Sanitation Program was developed in the 1960s when very little information on the structural and toxicological properties of brevetoxins in algae and shellfish was available. Alternative methods to mouse bioassay based on current scientific knowledge in the area are needed for monitoring NSP toxins. It is now established that brevetoxins are metabolized extensively in shellfish. Algal brevetoxins undergo oxidation and reduction, as well as conjugation with fatty acids and amino acids in shellfish. Recently, three metabolites have been identified as biomarkers of brevetoxin exposure and toxicity in Eastern oyster (Crassostrea virginica) and hard clam (Mercenaria sp.). The role of these biomarkers in monitoring NSP toxins in K. brevis exposed molluscan shellfish is reviewed. Comparisons of biomarker levels by liquid chromatography-mass spectrometry (LC-MS) with composite toxin as measured by enzyme linked immunosorbent assay (ELISA), and shellfish toxicity by mouse bioassay, support the application of these biomarkers as a dynamic and powerful approach for monitoring brevetoxins in shellfish and prevention of NSP.

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Development and Utilization of a Fluorescence-Based Receptor-Binding Assay for the Site 5 Voltage-Sensitive Sodium Channel Ligands Brevetoxin and Ciguatoxin

Cited by 37 publications

References 0 publications

An Updated Review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management

An Updated Review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management

Uptake and localization of fluorescently‐labeled Karenia brevis metabolites in non‐toxic marine microbial taxa

Role of Biomarkers in Monitoring Brevetoxins in <i>Karenia brevis</i> Exposed Shellfish

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