Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis

Abstract

Background: Human gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human gnathostomiasis. Findings: The recombinant protein (named rGslic18) was applied to ICT kit as the antigen. The diagnostic value of ICT kit was evaluated using serum samples from parasitologically proven and clinically suspected gnathostomiasis patients, healthy volunteers and patients with other parasitic diseases. The ICT kit exhibited quite high sensitivity (93.75 %) and specificity (97.01 %). Conclusions: The ICT kit is simple, convenient and easy to implement and expected to provide reliable diagnostic results for human gnathostomiasis. It also will be a promising diagnostic tool not only for large-scale epidemiological surveys in endemic or remote areas where diagnostic facilities are poor but also for a rapid clinical diagnosis in the bedside laboratory.

Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Serodiagnosis of COVID-19

“…13 − 15 The immunochromatographic method is regarded as a rapid and convenient method widely used in a variety of diseases, including infectious diseases. 16 , 17 Its principle is simple and easily accepted by doctors; these advantages make it especially suitable for a serious epidemic situation. In the current study, a sensitive immunofluorescent assay method was developed for rapid detection of special IgM and IgG of COVID-19 in human serum or plasma within 10 min.…”

A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis

“…There are two forms of rapid tests (lateral-flow and flow-through), with the advantages of low-cost, speed and convenience. A lateral-flow immunochromatographic test (ICT) for gnathostomiasis, with high sensitivity (93.75%) and specificity (97.01%), has recently been developed using a recombinant protein (rGslic18) as the antigen (Janwan et al ., 2016). In the present study, the DIGFA rapid test, in flow-through format, was developed and evaluated for gnathostomiasis.…”

Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages

“…The most known format consists of immobilizing an antigen (specific for a given pathogen) in the analytical zone and labeling an immunoglobulin-binding reagent-anti-species antibodies, bacterial immunoglobulin-binding proteins (protein A, protein G), etc. [ 24 , 25 , 26 , 27 , 28 , 29 ]. The presence of specific antibodies to the pathogen in the sample leads to the formation of a detectable complex of immobilized antigen molecules, specific antibodies and labeled immunoglobulin-binding protein ( Figure 1 A).…”