Development and quantitative characterization of the precursor rheology of hyaluronic acid hydrogels for bioprinting

Kiyotake, Emi A.; Douglas, Alexander W.; Thomas, Emily E.; Nimmo, Susan L.; Detamore, Michael S.

doi:10.1016/j.actbio.2019.01.041

Cited by 129 publications

(137 citation statements)

References 45 publications

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“…Rheological characteristics such as viscosity may vary depending on the degree of polymerization of HA, but by using ND, it was possible to form a viscosity suitable for printing despite the use of a lower content of HA. Compared to the results obtained by measuring the viscosity using a commercially available product (Cellink®) and pentenoatefunctionalized HA, viscosity in 2/s was 52.8 Pa•s for HA/ND-COOH (at pH 8), about 100 Pa•s for Cellink®, and 120 Pa•s for 3% pentanoate functionalized HA [35]. Compared to the results of other studies, the viscosity was relatively low, but concentration of hyaluronic acid (2 wt %) was lower than other hydrogel (at least 3%), and HA/ND hydrogel showed stronger shear thinning effect than other materials.…”

Section: Resultscontrasting

confidence: 55%

“…In addition, HA is easy to perform chemical modi cation, which can modify the physicochemical properties [34]. Due to the above advantages, various studies on chemically modifying HA as bioink for 3d printing have been conducted such as pentenoate-functionalized HA [35], βcyclodextrin /adamantane induced HA [36,37]. In this study, commonly used crosslinkable methacrylated HA was used for nanocomposite hydrogel with addition of nanodiamonds for 3D processable printing.…”

Section: Introductionmentioning

confidence: 99%

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pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

Lim

Kang

Jeong

2020

Preprint

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Nanocomposite hydrogels capable of undergoing manufacturing process have recently attracted attention in biomedical applications due to their desired mechanical properties and high functionality. 3D printing nanocomposite hydrogels of hyaluronic acid (HA)/nanodiamond (ND) revealed that the addition of ND with the low weight ratio of 0.02 wt % resulted in higher compressive force and gel breaking point, compared with HA only nanocomposites. These HA nanocomposite hydrogels loaded with surface functionalized ND allowed for the enforced compressive stress to be tuned in a pH-dependent manner. HA nanocomposite hydrogels with ND-OH at pH 8 showed an increase of 1.40 fold (0.02%: 236.18 kPa) and 1.37 fold (0.04%: 616.72 kPa) the compressive stress at the composition of 0.02 wt % and 0.04 wt, respectively, compared to those of ND-COOH (0.02%: 168.31 kPa, 0.04%: 449.59 kPa) at the same pH. Moreover, the compressive stress of HA/ND-OH (0.04 wt %) at pH 8 was mechanically enhanced 1.29 fold, compared to that of HA/ND-OH (0.04 wt %) at pH 7. These results indicate that the tunable buffering environment and interaction with the long chains of HA at the molecular level have a critical role in the dependency of the mechanical properties on pH. Due to the pH stability of the ND-OH nanophase, filament-based processing and layer-based deposition at microscale attained enforced mechanical properties of hydrogel. Fine surface tuning of the inorganic ND nanophase and controlled 3D printing leads to improved control over the pH-dependent mechanical properties of the nanocomposite hydrogels reported herein.

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Section: Resultscontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

Lim

Kang

Jeong

2020

Preprint

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“…The printability window is often predicted by bioink rheological properties such as viscosity, shear thinning, and recovery behavior to establish optimal printing parameters for the application [35]. Although the analysis of these properties is critical for understanding the parameters that guide bioink optimization, they are insufficient to determine printability [34,36]. The characterization of bioink shape fidelity after printing is needed to assess physical deformation of the printed filament and degree of similarity to the CAD design [28].…”

Section: Alginate-based Bioinks With Similar Viscosity and Thixotropimentioning

confidence: 99%

Evaluation of Alginate-Based Bioinks for 3D Bioprinting, Mesenchymal Stromal Cell Osteogenesis, and Application for Patient-Specific Bone Grafts

Fernández

Tenorio

Campbell

et al. 2020

Preprint

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To realize the promise of 3D bioprinting, it is imperative to develop bioinks that possess the biological and rheological characteristics needed for the printing of cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through pre-crosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate's physicochemical characteristics using common crosslinking agents can affect its cytocompatibility. Therefore, we evaluated the printability, physicochemical properties, and osteogenic potential of four common alginate bioinks: alginate-CaCl2 (alg-CaCl2), alginate-CaSO4 (alg-CaSO4), alginate-gelatin (alg-gel) and alginate-nanocellulose (alg-ncel) for the 3D bioprinting of patient-specific osteogenic grafts. While all bioinks possessed similar viscosity, printing fidelity was lower in the pre-crosslinked bioinks. When used to print geometrically defined constructs, alg-CaSO4 and alg-ncel exhibited higher mechanical properties and lower mesh size than those printed with alg-CaCl2 or alg-gel. The physical properties of these constructs affected the biological performance of encapsulated bone marrow-derived mesenchymal stromal cells (MSCs). Cell-laden constructs printed using alg-CaSO4 and alg-ncel exhibited greater cell apoptosis and contained fewer living cells 7 days post-printing. In addition, effective cell-matrix interactions were only observed in alg-CaCl2 printed constructs. When cultured in osteogenic media, MSCs in alg-CaCl2 constructs exhibited increased osteogenic differentiation compared to the other three bioinks. This bioink was then used to 3D print anatomically accurate cell-laden scaphoid bones that were capable of partial mineralization after 14 days of in vitro culture. These results highlight the importance of bioink properties to modulate cell behavior and the biofabrication of clinically relevant bone tissues.

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“…In extrusion printing, the rheology of an ink greatly determines printability [32][33][34]. We have conducted a detailed rheological characterization of our zein ink formulations at the time of preparation and after 21 days of aging.…”

Section: Rheology Characterizationmentioning

confidence: 99%

Biofabrication using maize protein: 3D printing using zein formulations

Negrete

Aceves-Colin

Rivera-Flores

et al. 2020

Preprint

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The use of three-dimensional (3D) printing for biomedical applications has expanded exponentially in recent years. However, the current portfolio of 3D printable inks is still limited. For instance, only a few protein matrices have been explored as printing/bioprinting materials. Here, we introduce the use of zein, the primary constitutive protein in maize seeds, as a 3D-printable material. Zein-based inks were prepared by dissolving commercial zein powder in ethanol with or without polyethylene glycol (PEG400) as a plasticizer. The rheological characteristics of our materials, studied during 21 days of aging/maturation, showed an increase in the apparent viscosity as a function of time in all formulations. The addition of PEG 400 decreased the apparent viscosity. Inks with and without PEG400 and at different maturation times were tested for printability in a BioX bioprinter. We optimized the 3D printing parameters for each ink formulation in terms of extrusion pressure and linear printing velocity. Higher fidelity structures were obtained with inks that had maturation times of 10 to 14 days. We present different proof-of-concept experiments to demonstrate the versatility of the engineered zein inks for diverse biomedical applications. These include printing of complex and/or free-standing 3D structures, materials for controlled drug release, and scaffolds for cell culture.

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Development and quantitative characterization of the precursor rheology of hyaluronic acid hydrogels for bioprinting

Cited by 129 publications

References 45 publications

pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

pH-Dependent Nanodiamonds Enhance the Mechanical Properties of 3D-Printed Hyaluronic Acid Nanocomposite Hydrogels

Evaluation of Alginate-Based Bioinks for 3D Bioprinting, Mesenchymal Stromal Cell Osteogenesis, and Application for Patient-Specific Bone Grafts

Biofabrication using maize protein: 3D printing using zein formulations

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