Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration

Abstract

Bone tissue damages are one of the dominant causes of temporary disability and developmental disability. Currently, there are some methods of guided bone regeneration employing different osteoplastic materials and insulation membranes used in surgery. In this study, we have developed a method of preparation of porous membranes from the biopolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), produced by a strain of Azotobacter chroococcum 7B. The biocompatibility of the porous membranes was investigated in vitro using mesenchymal stem cells (MSCs) and in vivo on laboratory animals. The cytotoxicity test showed the possibility of cell attachment on membrane and histological studies confirmed good insulating properties the material. The data obtained demonstrate the high biocompatibility and the potential application of insulating membranes based on PHBV in bone tissue engineering.

Application of Polyhydroxyalkanoates in Medicine and the Biological Activity of Natural Poly(3-Hydroxybutyrate)

“…It was demonstrated that PHB-based products (non-woven patches, porous scaffolds) facilitate the regeneration of tissues in different organs: osseous, cardiac and vascular, neural, and intestinal tissues. Application of PHB-based devices causes a high degree of vascularization in the area of tissue defect repair [23-28, 32, 35, 65]. It was shown using the critical (the parietal region of rat skull) and noncritical (rat femur) models of bone defects that PHB-based porous scaffolds facilitate bone tissue regeneration.…”

“…Expression of osteogenic markers (e.g., type I collagen) is also indicative of bone tissue regeneration in a PHB-based scaffold [23, 24]. We observed a uniform formation of nascent bone tissue over the entire volume of the porous biopolymeric scaffold in the form of islets rather than on the edges; meanwhile, a fibrous capsule did not form around the biopolymeric material, an indication of its complete integration with the bone tissue [24, 65]. All this demonstrates that PHB exhibits excellent biocompatibility with bone tissue and possess an osteoconductive and even osteoinductive potential.…”

Innovative methods for optimization of bone regeneration: mesenhymal bone cells (part 2) (literature review)

“…Культивируют МСК, чтобы увеличить их количество для последующей трансплантации. Их используют для оптимизации регенерации кости [71][72][73], а также в качестве тест-систем для определения биосовместимости и цитотоксичности остеопластических материалов [74]. На основе культивирования МСК создана методика определения регенеративного потенциала костной ткани человека [75,76], опираясь на результаты которой можно подобрать индивидуальное лечение для каждого пациента.…”

Development and Preclinical Studies of Orthotopic Bone Implants Based on a Hybrid Construction from Poly(3-Hydroxybutyrate) and Sodium Alginate

“…Such hybrid scaffold construction based on PHB filled with sodium alginate was developed with account of previously obtained data [17,18]. We have established that barrier membrane of PHB and the paste for filling bone defects based on microspheres of PHB in alginate gel are effective for bone tissue regeneration.…”

“…In studying regeneration of cranial bones the model of critical skullcap defect (parietal bone) in rats is the most revealing [20] as it allows obtaining reproducible data and comparing them with numerous results of other investigations [8,[16][17][18]. Such model is used to evaluate efficacy and security of various bone-replacing materials, including scaffolds with growth factors and cells [21][22][23].…”