Development and persistence of neuropathic pain through microglial activation and KCC2 decreasing after mouse tibial nerve injury

Kosaka, Yoshinori; Yafuso, Tsukasa; Shimizu-Okabe, Chigusa; Kim, Jeong-Tae; Kobayashi, Shiori; Okura, Nobuhiko; Ando, Hironobu; Okabe, Akihito; Takayama, Chitoshi

doi:10.1016/j.brainres.2020.146718

Cited by 7 publications

(5 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spinal and brain microglia play an important role in various neuropathic pain states [49][50][51][52][53][54]. Previously, it was demonstrated that the expression of astrocytes and microglia was increased in the anterior cingulate cortex of nerve-ligated mice.…”

Section: Discussionmentioning

confidence: 99%

The Microglial Activation Inhibitor Minocycline, Used Alone and in Combination with Duloxetine, Attenuates Pain Caused by Oxaliplatin in Mice

2021

View full text Add to dashboard Cite

The antitumor drug, oxaliplatin, induces neuropathic pain, which is resistant to available analgesics, and novel mechanism-based therapies are being evaluated for this debilitating condition. Since activated microglia, impaired serotonergic and noradrenergic neurotransmission and overexpressed sodium channels are implicated in oxaliplatin-induced pain, this in vivo study assessed the effect of minocycline, a microglial activation inhibitor used alone or in combination with ambroxol, a sodium channel blocker, or duloxetine, a serotonin and noradrenaline reuptake inhibitor, on oxaliplatin-induced tactile allodynia and cold hyperalgesia. To induce neuropathic pain, a single dose (10 mg/kg) of intraperitoneal oxaliplatin was used. The mechanical and cold pain thresholds were assessed using mouse von Frey and cold plate tests, respectively. On the day of oxaliplatin administration, only duloxetine (30 mg/kg) and minocycline (100 mg/kg) used alone attenuated both tactile allodynia and cold hyperalgesia 1 h and 6 h after administration. Minocycline (50 mg/kg), duloxetine (10 mg/kg) and combined minocycline + duloxetine influenced only tactile allodynia. Seven days after oxaliplatin, tactile allodynia (but not cold hyperalgesia) was attenuated by minocycline (100 mg/kg), duloxetine (30 mg/kg) and combined minocycline and duloxetine. These results indicate a potential usefulness of minocycline used alone or combination with duloxetine in the treatment of oxaliplatin-induced pain.

show abstract

Section: Discussionmentioning

confidence: 99%

The Microglial Activation Inhibitor Minocycline, Used Alone and in Combination with Duloxetine, Attenuates Pain Caused by Oxaliplatin in Mice

2021

View full text Add to dashboard Cite

show abstract

“…Indeed, nerve injury decreases the function and expression of KCC2 in neurons of lamina I of the superficial dorsal horn, events that are associated with the development of pain behaviors. 12,38 We observed that nerve injury decreased a 6 subunit protein expression in DRG and spinal cord for at least 21 days. These results suggest that neuropathic pain could result not only from the reduction of KCC2 but also from the downregulation of the a 6 -containing GABA A receptor at the DRG and deep dorsal horn.…”

Section: Discussionmentioning

confidence: 88%

Activation of α6-containing GABAA receptors induces antinociception under physiological and pathological conditions

Rodríguez‐Palma

Luz-Cuellar

Islas-Espinoza

et al. 2022

Pain

View full text Add to dashboard Cite

show abstract

“…Tactile allodynia is caused when neurons in the pain sensing pathway have a decrease in chloride (Cl − ) efflux. This decreased efflux results in a higher intracellular Cl − concentration, which in turn changes the neurons' activity and causes normally painless touch to become painful (Kosaka et al, 2020; Lu et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

How students reason about matter flows and accumulations in complex biological phenomena: An emerging learning progression for mass balance

et al. 2022

View full text Add to dashboard Cite

In recent years, there has been a strong push to transform STEM education at K‐12 and collegiate levels to help students learn to think like scientists. One aspect of this transformation involves redesigning instruction and curricula around fundamental scientific ideas that serve as conceptual scaffolds students can use to build cohesive knowledge structures. In this study, we investigated how students use mass balance reasoning as a conceptual scaffold to gain a deeper understanding of how matter moves through biological systems. Our aim was to lay the groundwork for a mass balance learning progression in physiology. We drew on a general models framework from biology and a covariational reasoning framework from math education to interpret students' mass balance ideas. We used a constant comparative method to identify students' reasoning patterns from 73 interviews conducted with undergraduate biology students. We helped validate the reasoning patterns identified with >8000 written responses collected from students at multiple institutions. From our analyses, we identified two related progress variables that describe key elements of students' performances: the first describes how students identify and use matter flows in biology phenomena; the second characterizes how students use net rate‐of‐change to predict how matter accumulates in, or disperses from, a compartment. We also present a case study of how we used our emerging mass balance learning progression to inform instructional practices to support students' mass balance reasoning. Our progress variables describe one way students engage in three dimensional learning by showing how student performances associated with the practice of mathematical thinking reveal their understanding of the core concept of matter flows as governed by the crosscutting concept of matter conservation. Though our work is situated in physiology, it extends previous work in climate change education and is applicable to other scientific fields, such as physics, engineering, and geochemistry.

show abstract

Development and persistence of neuropathic pain through microglial activation and KCC2 decreasing after mouse tibial nerve injury

Cited by 7 publications

References 118 publications

The Microglial Activation Inhibitor Minocycline, Used Alone and in Combination with Duloxetine, Attenuates Pain Caused by Oxaliplatin in Mice

The Microglial Activation Inhibitor Minocycline, Used Alone and in Combination with Duloxetine, Attenuates Pain Caused by Oxaliplatin in Mice

Activation of α6-containing GABAA receptors induces antinociception under physiological and pathological conditions

How students reason about matter flows and accumulations in complex biological phenomena: An emerging learning progression for mass balance

Contact Info

Product

Resources

About