Development and performance evaluation of a three-dimensional clinostat synchronized heavy-ion irradiation system

Ikeda, Hiroko; Souda, Hikaru; Puspitasari, Anggraeini; Held, Kathryn D.; Hidema, Jun; Nikawa, Takeshi; Yoshida, Yukari; Kanai, Tatsuaki; Takahashi, Akio

doi:10.1016/j.lssr.2017.01.003

Cited by 24 publications

(32 citation statements)

References 36 publications

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“…Currently, there are a number of microgravity devices available for purchase that are designed to achieve microgravity conditions. Microgravity simulators specific to cellular studies include strong magnetic field-induced levitation (i.e., diamagnetic simulation), as well as two-dimensional and three-dimensional clinostats, rotating wall vessels and random positioning machines (RPMs) (Huijser, 2000;Russomano et al, 2005;Herranz et al, 2012;Ikeda et al, 2017). Each of the simulation techniques has shown advantages and disadvantages however, when chosen correctly for a given experiment, the results obtained are similar to those observed in Space flight studies (Stamenkovic et al, 2010;Herranz et al, 2013;Martinez et al, 2015;Krüger et al, 2019b).…”

Section: Simulating Microgravitymentioning

confidence: 90%

Modeling the Impact of Microgravity at the Cellular Level: Implications for Human Disease

Bradbury

Choi

et al. 2020

Front. Cell Dev. Biol.

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A lack of gravity experienced during space flight has been shown to have profound effects on human physiology including muscle atrophy, reductions in bone density and immune function, and endocrine disorders. At present, these physiological changes present major obstacles to long-term space missions. What is not clear is which pathophysiological disruptions reflect changes at the cellular level versus changes that occur due to the impact of weightlessness on the entire body. This review focuses on current research investigating the impact of microgravity at the cellular level including cellular morphology, proliferation, and adhesion. As direct research in space is currently cost prohibitive, we describe here the use of microgravity simulators for studies at the cellular level. Such instruments provide valuable tools for cost-effective research to better discern the impact of weightlessness on cellular function. Despite recent advances in understanding the relationship between extracellular forces and cell behavior, very little is understood about cellular biology and mechanotransduction under microgravity conditions. This review will examine recent insights into the impact of simulated microgravity on cell biology and how this technology may provide new insight into advancing our understanding of mechanically driven biology and disease.

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Section: Simulating Microgravitymentioning

confidence: 90%

Modeling the Impact of Microgravity at the Cellular Level: Implications for Human Disease

Bradbury

Choi

et al. 2020

Front. Cell Dev. Biol.

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“…However, there are two major limitations associated with this approach: (i) it is necessary to stop rotation during irradiation as the sample was exposed to radiation outside the incubator after or before rotation with a RWV [183][184][185][186][187][188] and (ii) nonuniformity of dose flatness in the irradiation area occurs because of chronical irradiation of a rotating sample with a RPM [189,190]. To address these problems, we have developed a system of simultaneous irradiation in simulated-μG (SSS) using 3D clinostat [191,192]. Our SSS is based on technologies related to X-ray irradiation with a high-speed shutter [191] and Cion radiotherapy such as accelerator systems and respiratory gating systems [192].…”

Section: Combined Biological Effectsmentioning

confidence: 99%

“…To address these problems, we have developed a system of simultaneous irradiation in simulated-μG (SSS) using 3D clinostat [191,192]. Our SSS is based on technologies related to X-ray irradiation with a high-speed shutter [191] and Cion radiotherapy such as accelerator systems and respiratory gating systems [192].…”

Section: Combined Biological Effectsmentioning

confidence: 99%

Space Radiation Biology for “Living in Space”

Furukawa

Nagamatsu

Nenoi

et al. 2020

BioMed Research International

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Space travel has advanced significantly over the last six decades with astronauts spending up to 6 months at the International Space Station. Nonetheless, the living environment while in outer space is extremely challenging to astronauts. In particular, exposure to space radiation represents a serious potential long-term threat to the health of astronauts because the amount of radiation exposure accumulates during their time in space. Therefore, health risks associated with exposure to space radiation are an important topic in space travel, and characterizing space radiation in detail is essential for improving the safety of space missions. In the first part of this review, we provide an overview of the space radiation environment and briefly present current and future endeavors that monitor different space radiation environments. We then present research evaluating adverse biological effects caused by exposure to various space radiation environments and how these can be reduced. We especially consider the deleterious effects on cellular DNA and how cells activate DNA repair mechanisms. The latest technologies being developed, e.g., a fluorescent ubiquitination-based cell cycle indicator, to measure real-time cell cycle progression and DNA damage caused by exposure to ultraviolet radiation are presented. Progress in examining the combined effects of microgravity and radiation to animals and plants are summarized, and our current understanding of the relationship between psychological stress and radiation is presented. Finally, we provide details about protective agents and the study of organisms that are highly resistant to radiation and how their biological mechanisms may aid developing novel technologies that alleviate biological damage caused by radiation. Future research that furthers our understanding of the effects of space radiation on human health will facilitate risk-mitigating strategies to enable long-term space and planetary exploration.

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“…To allow the assessment and management of human health risks in space, it is necessary to obtain more basic data on the combined effects of radiation under microgravity [30,65]. To address these serious problems, we developed 3-dimensional clinostat-synchronized heavy-ion and x-ray irradiation systems [66,67], which are expected to provide significant contributions to space radiation research, as a valuable platform for studies on the relative biological effectiveness and the combined effects of radiation under microgravity.…”

Section: Human Effect Of Space Radiationmentioning

confidence: 99%

Role of High-Linear Energy Transfer Radiobiology in Space Radiation Exposure Risks

Takahashi

Ikeda

Yoshida

2018

International Journal of Particle Therapy

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Many manned missions to the Moon and Mars are scheduled in the near future. However, space radiation presents a major hazard to humans, and astronauts are constantly exposed to radiation, including high linear energy transfer (LET) radiation, which differs from radiation on Earth. Thus, there is thus an urgent need to clarify the biological effects of space radiation and reduce the associated risks. In this review, we consider the role of high-LET radiobiology in relation to space-radiation exposure.

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Development and performance evaluation of a three-dimensional clinostat synchronized heavy-ion irradiation system

Cited by 24 publications

References 36 publications

Modeling the Impact of Microgravity at the Cellular Level: Implications for Human Disease

Modeling the Impact of Microgravity at the Cellular Level: Implications for Human Disease

Space Radiation Biology for “Living in Space”

Role of High-Linear Energy Transfer Radiobiology in Space Radiation Exposure Risks

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