The effects of skipjack tuna (Katsuwonus pelamis) spleen addition at various levels (0, 10 and 20%) on the liquefaction and characteristics of sardine fish sauce produced with different salt concentrations (15, 20 and 25%) were monitored during fermentation of 180 days. Fish sauce prepared from sardine with spleen supplementation contained greater total nitrogen, amino nitrogen, formaldehyde nitrogen, ammonia nitrogen content, compared to those without spleen addition throughout the fermentation. The rate of liquefaction was dependent upon the amount of spleen added. Reduction of salt content accelerated the hydrolysis of fish protein during fermentation. The liquefaction rate of the lower salt treated samples was generally faster than those treated with higher salt content. Among all treatments, sardine added with 25% spleen and 15% salt exhibited the greatest protein hydrolysis, particularly at early stages, suggesting the combination effects of autolysis and spleen proteinase. The greater liquefaction was coincidental with the development of browning as well as the increase in redness of liquid formed. Acceptability test revealed that the samples were different in color, aroma, taste as well as overall acceptance (p<0.05). Fish sauce samples containing 20% salt without and with 10% spleen addition had the similar acceptability with commercial fish sauce. Therefore, addition of spleen as well as salt reduction can accelerate the liquefaction of sardine for fish sauce production.