Development and optimization of sequence‐tagged microsatellite site markers to detect genetic diversity within <i>Colletotrichum capsici,</i> a causal agent of chilli pepper anthracnose disease

Ranathunge, N. P.; Ford, Rebecca; Taylor, P. W. J.

doi:10.1111/j.1755-0998.2009.02608.x

Cited by 21 publications

(28 citation statements)

References 12 publications

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“…Very few studies have been conducted on the causative agent of Citrus anthracnose disease worldwide (Keuete Kamdoum, Noumbo, & Kuiate, ). Understanding pathogen population structure, its spatio‐temporal dynamics and its genetic diversity leading to host adaptation and fungicide resistance are essential for the development of effective disease management strategies (Mahmodi et al, ; Marulanda et al, ; Moges et al, ; Rampersad, ; Ranathunge et al, ). The objectives of the study were (a) to identify the causative agent of anthracnose disease, associated with twig wither‐tip symptoms, across major Citrus growing areas in Tunisia and (b) to analyse the genetic diversity and population structure of Colletotrichum sp.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity ofColletotrichum gloeosporioidesspecies complex associated withCitruswither‐tip of twigs in Tunisia using microsatellite markers

Bahri

Saadani

Mechichi

et al. 2019

Journal of Phytopathology

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Anthracnose Citrus disease has been associated with several symptoms worldwide and it is recently compromising Citrus production in the Mediterranean area. Four species complexes are mainly involved: Colletotrichum boninense, C. acutatum, C. gloeosporioides and C. truncatum. In this study, we investigated the genetic diversity of Colletotrichum spp. in Tunisia associated with wither‐tip of twigs on Citrus. Specific primers ITS4‐CgInt allowed the identification of C. gloeosporioides species complex in all the 54 isolates, sampled from three regions and four Citrus species. Overall, our genotypic analysis using 10 SSR markers showed a moderate diversity level in Tunisian C. gloeosporioides population and highlighted that C. gloeosporioides reproduce mainly clonally. In addition, heterothallic isolates were present in our population, suggesting that the pathogen population may undergo parasexual recombinations. The highest genetic diversity in C. gloeosporioides was recorded in Nabeul and on orange, which likely constitutes the area and the host of origin for the Citrus anthracnose disease in Tunisia. In addition, no population subdivision was detected at the geographic, host species or cultivars’ origin levels. However, our study identified two genetic subpopulations and indicated a rapid C. gloeosporioides population change at temporal scale that should be further examined over several consecutive growing seasons in order to understand its population dynamics.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity ofColletotrichum gloeosporioidesspecies complex associated withCitruswither‐tip of twigs in Tunisia using microsatellite markers

Bahri

Saadani

Mechichi

et al. 2019

Journal of Phytopathology

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“…), and microsatellite (Ranathunge et al. ; Rampersad ; Sharma et al. ) markers to analyze strains of C. truncatum and other Colletotrichum species.…”

Section: Introductionmentioning

confidence: 99%

“…Ranathunge et al. () developed 27 microsatellite markers and determined the diversity of 52 C. truncatum isolates from India, Sri Lanka, and Thailand. For Colletotrichum graminicola , random amplified polymorphic DNA (RAPD) marker analysis of C. graminicola isolates from turf grass revealed a high degree of genetic similarity among isolates recovered from the same host (Browning et al.…”

Section: Introductionmentioning

confidence: 99%

Genetic differentiation and recombination among geographic populations of the fungal pathogen Colletotrichum truncatum from chili peppers in China

Diao

Zhang

et al. 2014

Evolutionary Applications

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Colletotrichum truncatum is an extremely important fungal pathogen. It can cause diseases both in humans and in over 460 plant species. However, little is known about its genetic diversity within and among populations. One of the major plant hosts of C. truncatum is pepper, and China is one of the main pepper-producing countries in the world. Here, we propose the hypotheses that geography has a major influence on the relationships among populations of C. truncatum in China and that infections in different populations need to be managed differently. To test these hypotheses, we obtained and analyzed 266 C. truncatum isolates from 13 regions representing the main pepper-growing areas throughout China. The analysis based on nine microsatellite markers identified high intrapopulation genetic diversity, evidence of sexual recombination, and geographic differentiation. The genetic differentiation was positively correlated with geographic distance, with the southern and northern China populations grouped in two distinct clusters. Interestingly, isolates collected from the pepper-breeding center harbored the most private alleles. The results suggest that the geographic populations of C. truncatum on peppers in China are genetically differentiated and should be managed accordingly. Our study also provides a solid foundation from which to further explore the global genetic epidemiology of C. truncatum in both plants and humans.

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“…In studies conducted by Ranathunge et al (2009), the number of alleles was higher in 27 primer pairs developed and evaluated for another species of the same genus, C. capsici. Locus CCSSR1 showed 18 alleles detected across the 52 isolates and several common alleles within groups of isolates from India, Sri Lanka, and Thailand.…”

Section: Characterizing Colletotrichum Populationsmentioning

confidence: 99%

“…One such study was that conducted by Ranathunge et al (2009) to obtain SSRs of the causal agent of anthracnose in red pepper (Capsicum spp), using 27 microsatellites to characterize 52 isolates from different regions of India, Sri Lanka, and Thailand. Recently, microsatellite markers were also developed and applied to study isolates and populations of several phytopathogenic fungi, such as Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans, Magnaporthe grisea, and Ascochyta rabiei (Ciampi et al, 2008;Ranathunge et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite isolation and characterization for Colletotrichum spp, causal agent of anthracnose in Andean blackberry

et al. 2014

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ABSTRACT. The genus Colletotrichum, comprised of pathogenic fungi that affect plants grown worldwide, causes the disease known as anthracnose in several fruit and vegetable species. Several studies conducted on plants have shown that the disease is characterized by the presence of one or several species of the fungus attacking the fruit or other organs of the same host. To develop and implement effective control strategies, it is vital to understand the genetic structure of the fungus in agricultural systems, identify associated Colletotrichum species, and define the subpopulations responsible for the disease. Molecular tools were accordingly developed to characterize genotypic populations of Colletotrichum spp, causal agent of anthracnose in commercial crops of Andean blackberry (Rubus glaucus Benth.). A microsatellite-enriched library for Colletotrichum gloeosporioides was developed to identify and characterize microsatellite loci among isolates collected in R. glaucus plantations. Thirty microsatellites were developed and tested in 36 isolates gathered from eight different blackberry-production areas of Colombia. Ten pairs of microsatellites were polymorphic.

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Development and optimization of sequence‐tagged microsatellite site markers to detect genetic diversity within Colletotrichum capsici, a causal agent of chilli pepper anthracnose disease

Cited by 21 publications

References 12 publications

Genetic diversity ofColletotrichum gloeosporioidesspecies complex associated withCitruswither‐tip of twigs in Tunisia using microsatellite markers

Genetic diversity ofColletotrichum gloeosporioidesspecies complex associated withCitruswither‐tip of twigs in Tunisia using microsatellite markers

Genetic differentiation and recombination among geographic populations of the fungal pathogen Colletotrichum truncatum from chili peppers in China

Microsatellite isolation and characterization for Colletotrichum spp, causal agent of anthracnose in Andean blackberry

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