Development and optimization of an immunoassay for the detection of Hg(II) in lake water

Abstract

In this paper, an indirect competitive enzyme‐linked immunosorbent assay (IC‐ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb‐A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween‐20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC 50 ) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti‐Hg mAbs also have some affinity with methyl mercury (CH 3 Hg) and with no cross‐reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.

Lead and cadmium biosorption from milk by Lactobacillus acidophilus ATCC 4356

“…Pb and Cd are the toxic heavy metals for humans that can be found in air, soil, water, and even food. They are in the risky heavy metals’ group (Bhakta, Ohnishi, Munekage, Iwasaki, & Wei, 2012; Jin et al., 2019). They are undegradable compounds, and their toxicity in the environment is known as the most dangerous pollutants in the world (Demirbas, 2008).…”

Non-Specific Binding and Cross-Reaction of ELISA: A Case Study of Porcine Hemoglobin Detection

“…It is recommended to combine the polymers with protein blockers to achieve the desired blocking effect [36]. Due to the high viscosity of the polymers, they are commonly used at a low concentration [56].…”

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

“…Due to the small molecular weight of Cd, we used a bifunctional chelator, ITCBE, as a bridge between proteins and Cd to synthesize the immunogen (Jin et al, 2019). The method ( Figure 1) was performed as reported by Song et al with some modifications (Song, Zou, Zhu, Liu, & Kuang, 2017).…”