Development and optimization of a reversed-phase high-performance liquid chromatographic method for the determination of acetaminophen and its major metabolites in rabbit plasma and urine after a toxic dose

Acetaminophen Improves Analgesia but Does Not Reduce Opioid Requirement After Major Spine Surgery in Children and Adolescents

“…Plasma concentrations of acetaminophen and its glucuronide and sulfate conjugates were determined by using a minor modifi cation of a previously published reversed-phase high-performance liquid chromatographic method. 16 In our method, plasma proteins were precipitated using methanol, instead of perchloric acid, to avoid the hydrolysis of acetaminophen sulfate in acidic medium. The lower limits of quantifi cation were 0.25 mg/L for acetaminophen and its sulfate and 0.5 mg/L for acetaminophen glucuronide.…”

The Preliminary Studies of Electrochemical Behavior of Paracetamol and Its Metabolites on Glassy Carbon Electrode by Voltammetric Methods

“…The high performance liquid chromatography with UV detection was used to analyze PAR, PG and PS in urine of HIVþ /AIDS patients [1], in the plasma and urine of rabbits [2], in biological samples of rats [3] and in the human and children urine, plasma and saliva [4,5]. Also liquid chromatography with MS detection is reported for the determination of these compounds in some biological fluids [6].…”

Human Hepatocyte Morphology and Functions in a Multibore Fiber Bioreactor

“…Mobile phase A (acetonitrile) and mobile phase B (2% H 3 PO 4 in water) were used in a step gradient program at 1 mL · min −1 : from 0 min to 10 min a linear change from 15% A and 85% B to 20% A and 80% B; from 10 min to 20 min , a linear change from 20% A and 80% B to 75% A and 25% B, and from 20 min to 25 min a linear change from 75% A and 25% B to 80% A and 20% B. The samples were diluted with acetonitrile (1:1, v/v) and after acidification with 0.1 mL of H 3 PO 4 , vortexing for 1 min and then centrifuged at 6 000 g for 10 min 21…”