Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of Potato Mop-Top Virus at Quarantine Inspection Sites in Korea

Abstract

- (PMTV) is classified as a plant quarantine virus in Korea. It is tested at import inspection sites. In this study, two sets of reverse transcription-hemi-nested polymerase chain reaction primers were developed to diagnose PMTV. In addition, modified- positive control plasmid was developed for the identification of false positive results in plant quarantine. The assay is expected to be useful for improving the detection and diagnosis of PMTV.

Development and Evaluation of a SYBR Green-Based, Real-time Polymerase Chain Reaction for Rapid and Specific Detection of Human Coxsackievirus B5

“…Jaianand et al (2011), Zhao et al (2013) (Hardinge and Murray, 2019;Schneider et al, 2019). Rezig et al (2004) (Lee et al, 2015;Lee et al, 2017). 한편 real-time PCR은 단일 과정으로 상대적으로 간편하고, 2시간 이내의 빠른 검출이 가능하며, nested PCR과 동등 이상 수준의 검출 민 감도를 가진 기법으로 최근 임상과 비 임상에서 주요 분 자진단 기법으로 활용되고 있다 (Chen et al, 2017;Yoo et al, 2017;Corman et al, 2020).…”