Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot

An, Diaoguo; Ma, Pengtao; Zheng, Qi; Fu, Shulan; Li, Lihui; Han, Fangpu; Han, Guohao; Wang, Jing; Xu, Yunfeng; Jin, Yuli; Zhang, Xiaotian

doi:10.1007/s00122-018-3214-3

Cited by 71 publications

(42 citation statements)

References 60 publications

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“…Recently, the exploitation of available genes in the wild relatives of wheat has diversified greatly. In particular, An et al [31] obtained a new wheat-rye addition line called WR35 via crossing Xiaoyan 6 with the rye cultivar German White, where the addition line exhibited resistance to powdery mildew and stripe rust, and a high kernel number per spike. Pan et al [32] also found that the 1P chromosome from Agropyron cristatum enhanced the spike length and tiller number in wheat, and a wheat-A.…”

Section: Discussionmentioning

confidence: 99%

“…Wheat disomic addition lines had substantial value in various applications as basic materials for investigating the genetic relationships between wheat and its relatives and gene mapping, they can also be employed as bridge materials for producing substitution lines and translocation lines [31]. A complete set of 1Ns-7Ns wheat-P. huashanica disomic addition lines was developed by Du et al [51].…”

Section: Discussionmentioning

confidence: 99%

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A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

Agronomy

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Powdery mildew is a fungal disease that threatens wheat production throughout the world. Breeding resistant cultivars is an effective way to reduce harm caused by powdery mildew. In this study, 35 wheat-Psathyrostachys huashanica-derived lines were developed by crossing common wheat and P. huashanica Keng (2n = 2x = 14, NsNs) using embryo culture. Resistance to powdery mildew in the derived lines was identified at the seedling and adult stages. Line H5-5-4-2 was selected with immunity to powdery mildew at both growth stages. The chromosome structure of this line was characterized by cytology, genomic in situ hybridization (GISH), and expressed sequence tag-sequence-tagged site (EST-STS) analysis. The chromosome configuration was 2n = 44 = 22II. Two P. huashanica chromosomes with strong hybridization signals were detected by GISH analysis. Among 83 EST-STS markers that covered all seven homologous groups in wheat, three pairs of STS markers, BE497584, BF202643, and BG262410, located in wheat homologous group 1 amplified clear specific bands related to P. huashanica. The results indicated that resistant line H5-5-4-2 was a wheat-P. huashanica 1Ns disomic addition line.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Liu¹,

Hou²,

Li³

et al. 2020

Agronomy

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“…Limited germplasm resistant to take-all disease has been identified in common wheat (McMillan et al, 2014). Chromosome engineering is important for crop breeders to develop new disease-resistant germplasm and broaden the genetic base of wheat (Jiang et al, 1993;Zhuang et al, 2015;An et al, 2019). Hollins et al (1986) found that the FIGURE 5 | The distribution ratio of differential SNP per Mb on 2Ns(2D) chromosome between in H139 and 16-6.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All

Bai¹,

Yuan²,

Zhang³

et al. 2020

Front. Plant Sci.

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Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng (P. huashania)-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. Sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (mc-FISH) analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D. Expressed sequence tag-sequence tagged site (EST-STS) marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line. In addition, the H139 line was shown to be cytologically stable with a dwarf phenotype and increased spikelet number. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding.

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“…The wheat 'Shaanyou 225 was used as a mildew-susceptible control in tests of powdery mildew resistance. The Bgt isolate E09 is a widely prevalent isolate, and was used to test powdery mildew resistance [43]. All of the above-mentioned materials were obtained from the College of Agronomy, Northwest A&F University, China.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

Wang

Long

Wang

et al. 2020

IJMS

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Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat–Ae. geniculata alien disomic substitution line W16998 was obtained from the BC1F8 progeny of a cross between the common wheat ‘Chinese Spring’ (CS) and Ae. geniculata Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST–STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization–genomic in situ hybridization (sequential FISH–GISH), and assessment of agronomic traits and powdery mildew resistance. During the anaphase of meiosis, these were evenly distributed on both sides of the equatorial plate, and they exhibited high cytological stability during the meiotic metaphase and anaphase. GISH analysis indicated that W16998 contained a pair of Ae. geniculata alien chromosomes and 40 common wheat chromosomes. One EST–STS marker and seven PLUG marker results showed that the introduced chromosomes of Ae. geniculata belonged to homoeologous group 7. Nullisomic–tetrasomic analyses suggested that the common wheat chromosome, 7A, was absent in W16998. FISH and sequential FISH–GISH analyses confirmed that the introduced Ae. geniculata chromosome was 7Mg. Therefore, W16998 was a wheat–Ae. geniculata 7Mg (7A) alien disomic substitution line. Inoculation of isolate E09 (Blumeria graminis f. sp. tritici) in the seedling stage showed that SY159 and W16998 were resistant to powdery mildew, indeed nearly immune, whereas CS was highly susceptible. Compared to CS, W16998 exhibited increased grain weight and more spikelets, and a greater number of superior agronomic traits. Consequently, W16998 was potentially useful. Germplasms transfer new disease-resistance genes and prominent agronomic traits into common wheat, giving the latter some fine properties for breeding.

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Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot

Cited by 71 publications

References 60 publications

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Characterization of the Wheat-Psathyrostachys huashania Keng 2Ns/2D Substitution Line H139: A Novel Germplasm With Enhanced Resistance to Wheat Take-All

Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

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