Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides

Guo, Yue; Liu, Changle; Long, Wei; Gao, Jianqin; Zhang, Jiefu; Chen, Song; Huang, Pu; Hu, Meijiao

doi:10.1016/j.cj.2021.05.006

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…This mutant has two mutations: in genome A, BnAHAS3R (Trp574Leu), inherited from the M342 mutant, and in genome C, BnAHAS1-3R (Pro197Leu), obtained by chemical mutagenesis of seeds using EMS. The calculated 50% phytotoxicity values for the DS3 mutant doses were 753.2 g/ha of TBM and 669.8 g/ha of MES, whereas for the M342 mutant, these doses were 210.6 g/ha of TBM and 179.1 g/ha of MES [38]. The DS3 mutant was not treated with other herbicide groups in the present study.…”

Section: Mutants and Mutations Conferring Resistance To Imidazolinonesmentioning

confidence: 63%

“…Another DS3 mutant was obtained by mutagenesis of the M342 mutant using EMS, as reported by researchers Guo et al [38]. This mutant has two mutations: in genome A, BnAHAS3R (Trp574Leu), inherited from the M342 mutant, and in genome C, BnAHAS1-3R (Pro197Leu), obtained by chemical mutagenesis of seeds using EMS.…”

Section: Mutants and Mutations Conferring Resistance To Imidazolinonesmentioning

confidence: 90%

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Imidazolinone Resistance in Oilseed Rape (Brassica napus L.): Current Status, Breeding, Molecular Markers and Prospects for Application in Hybrid Seed Purity Improvement

Kozar,

Domblides

2024

Horticulturae

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Resistance of agricultural crops to herbicides is an important topic that concerns many researchers. One of the most popular groups of herbicides is the imidazolinone group. Resistant forms of crops such as wheat (Triticum aestivum L.), sunflower (Helianthus annuus L.), corn (Zea mays L.), rice (Oryza sativa L.) and oilseed rape (Brassica napus L.) have been developed to this group of herbicides. All crops resistant to this group of herbicides have the commercial name Clearfield®. In this review, the information concerning oilseed rape resistance to the imidazolinone group of herbicides is collected and summarized; it will be useful for breeders and researchers engaged in this direction. This review touches upon the topic of mechanisms of oilseed rape resistance to imidazolinones. Mutation variants known to date, which provide resistance to this group of herbicides, are described, and known molecular markers of them are presented. Approaches to the selection of oilseed rape for resistance to the imidazolinone group of herbicides are mentioned. Various methods of utilizing imidazolinone resistance to improve the purity of hybrid seeds are also considered.

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Section: Mutants and Mutations Conferring Resistance To Imidazolinonesmentioning

confidence: 63%

Section: Mutants and Mutations Conferring Resistance To Imidazolinonesmentioning

confidence: 90%

Imidazolinone Resistance in Oilseed Rape (Brassica napus L.): Current Status, Breeding, Molecular Markers and Prospects for Application in Hybrid Seed Purity Improvement

Kozar,

Domblides

2024

Horticulturae

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“…The W574L substitution has been reported to confer resistance to ALS inhibitors in rapeseed, sunflower and cocklebur (Bernasconi et al, 1995;Hattori et al, 1995;Sala et al, 2012;Hu et al, 2017;Guo et al, 2020). Mutation at P197 also conferred good tolerance to sulfonylureas in Arabidopsis, rapeseed and wheat (Li et al, 2015;Chen et al, 2017;Zhang et al, 2019;Huang et al, 2020;Wu et al, 2020;Cheng et al, 2021;Guo et al, 2022). In addition, mutations at the sites of Ala122, Ala205 and Ser653 of ALS have been reported to confer resistance to ALS inhibitors (Tan et al, 2005;Murphy and Tranel, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Synergistic effects have also been shown for herbicide resistance in crops. In B. napus, 5N (BnALS1-2R, W574L; BnALS3R, W574L) and DS3 (BnALS1-3R, P197L; BnALS3R, W574L) showed stronger herbicide resistance than mutants with single-point mutations (Guo et al, 2020 andGuo et al, 2022). In soybean, Als1 (P197S) and Als2 (W574L) exhibited synergistic resistant effects to ALS herbicides, and the combination of Als1 and Als2 conferred stronger tolerance to SU (Walter et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of functional kompetitive allele-specific PCR markers for herbicide resistance in Brassica napus

Shi,

Yu,

et al. 2023

Front. Plant Sci.

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Effective weed control in the field is essential for maintaining favorable growing conditions and rapeseed yields. Sulfonylurea herbicides are one kind of most widely used herbicides worldwide, which control weeds by inhibiting acetolactate synthase (ALS). Molecular markers have been designed from polymorphic sites within the sequences of ALS genes, aiding marker-assisted selection in breeding herbicide-resistant rapeseed cultivars. However, most of them are not breeder friendly and have relatively limited application due to higher costs and lower throughput in the breeding projects. The aims of this study were to develop high throughput kompetitive allele-specific PCR (KASP) assays for herbicide resistance. We first cloned and sequenced BnALS1 and BnALS3 genes from susceptible cultivars and resistant 5N (als1als1/als3als3 double mutant). Sequence alignments of BnALS1 and BnALS3 genes for cultivars and 5N showed single nucleotide polymorphisms (SNPs) at positions 1676 and 1667 respectively. These two SNPs for BnALS1 and BnALS3 resulted in amino acid substitutions and were used to develop a KASP assay. These functional markers were validated in three distinct BC1F2 populations. The KASP assay developed in this study will be valuable for the high-throughput selection of elite materials with high herbicide resistance in rapeseed breeding programs.

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Comparative genome-wide analysis of circular RNAs in Brassica napus L.: target-site versus non-target-site resistance to herbicide stress

Guo,

Wang,

et al. 2024

Theor Appl Genet

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Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides

Cited by 7 publications

References 40 publications

Imidazolinone Resistance in Oilseed Rape (Brassica napus L.): Current Status, Breeding, Molecular Markers and Prospects for Application in Hybrid Seed Purity Improvement

Imidazolinone Resistance in Oilseed Rape (Brassica napus L.): Current Status, Breeding, Molecular Markers and Prospects for Application in Hybrid Seed Purity Improvement

Development and validation of functional kompetitive allele-specific PCR markers for herbicide resistance in Brassica napus

Comparative genome-wide analysis of circular RNAs in Brassica napus L.: target-site versus non-target-site resistance to herbicide stress

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