Development and maturation of thymic dendritic cells during human ontogeny

Savchenko, A.S.; Hasegawa, Go; Naito, Makoto

doi:10.1007/s00441-006-0202-8

Cited by 15 publications

(19 citation statements)

References 19 publications

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“…This is the first time that S100 immunoreactivity in the thymic medulla in both isolated and epithelial cells of Hassall's corpuscles has been reported. However, it is possible that a subset of these cells with a more dendritic morphology are similar to the S100-positive dendritic cells described by Uccini et al (1986) and Savchenko et al (2006). Savschenko et al showed that S100-positive dendritic cells are present in the corticomedullary region and the medulla of the human thymus at an early stage of gestation, suggesting that S100-positive cells are candidates for the thymic dendritic cell precursor.…”

Section: Discussionmentioning

confidence: 88%

Ontogeny of Intrinsic Innervation in the Human Thymus and Spleen

Anagnostou

Doussis-Anagnostopoulou

Tiniakos

et al. 2007

J Histochem Cytochem.

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S U M M A R YThe ontogeny of the innervation of human lymphoid organs has not been studied in detail. Our aim was to assess the nature and distribution of parenchymal nerves in human fetal thymus and spleen. We used the peroxidase immunohistochemical technique with antibodies specific to neuron-specific enolase (NSE), neurofilaments (NF), PGP9.5, S100 protein, and tyrosine hydroxylase (TH) and evaluated our results with image analysis. In human fetal thymus, NSE-, NF-, S100-, PGP9.5-, and TH-positive nerves were identified associated with large blood vessels from 18 gestational weeks (gw) onwards, increasing in density during development. Their branches penetrated the septal areas at 20 gw, reaching the cortex and the corticomedullary junction between 20 and 23 gw. Few nerve fibers were seen in the medulla in close association with Hassall's corpuscles. In human fetal spleen, NSE-, NF-, S100-, PGP9.5-, and TH-positive nerve fibers were localized in the connective tissue surrounding the splenic artery at 18 gw. Perivascular NSE-, NF-, S100-, PGP9.5-, and TH-positive nerve fibers were seen extending into the white pulp, mainly in association with the central artery and its branches, increasing in density during gestation. Scattered NSE-, NF-, S100-, PGP9.5-, and THpositive nerve fibers and endings were localized in the red pulp from 18 gw onward. The predominant perivascular distribution of most parenchymal nerves implies that thymic and splenic innervation may play an important functional role during intrauterine life. (J Histochem Cytochem 55:813-820, 2007)

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Section: Discussionmentioning

confidence: 88%

Ontogeny of Intrinsic Innervation in the Human Thymus and Spleen

Anagnostou

Doussis-Anagnostopoulou

Tiniakos

et al. 2007

J Histochem Cytochem.

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“…During early ontogeny (4 months), S100 + thymic dendritic cell precursors populate the corticomedullary border and medullary region of thymus. Other thymic dendritic cell markers such as CD1a and Langerin appear later (at 5 months gestation) [63]. In thymus of newborns S100-protein + cells, including interdigitating dendritic cells, were found in thymic medulla, at the corticomedullary junction, nearby and inside Hassall's corpuscles, but rarely in the cortex [64], [65].…”

Section: Discussionmentioning

confidence: 98%

Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

et al. 2014

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Recently, a role of the receptor for advanced glycation endproducts (RAGE) in myasthenia gravis was described. RAGE and its ligand high mobility group box 1 (HMGB1) play key roles in autoimmunity and cancer. To test whether these molecules are involved in patients with thymic abnormalities we applied immunohistochemical analysis in 33 cases of thymic epithelial tumors, comprising 27 thymomas and 6 thymic carcinomas, and 21 nonneoplastic thymuses. Both molecules were detected in neoplastic epithelial cells: RAGE staining was most intense in WHO type B2 thymomas and thymic carcinomas (p<0.001). HMGB1 nuclear staining was strongest in A and AB, and gradually less in B1 = B2>B3>thymic carcinoma (p<0.001). Conversely, HMGB1 cytoplasmic staining intensities were as follows: A and AB (none), B1 (strong), B2 (moderate), B3 and thymic carcinoma (weak); (p<0.001). Fetal thymic tissue showed a distinct expression of RAGE and HMGB1 in subcapsular cortical epithelial cells which was found in 50% of myasthenic patients. Furthermore RAGE and HMGB1 were expressed in thymocytes, macrophages, Hassall's corpuscles, thymic medulla, and germinal center cells in myasthenic patients. Immunohistochemistry results were complemented by systemic measurements (immunosorbent assay): serum levels of soluble RAGE were significantly reduced in patients with epithelial tumors (p = 0.008); and in invasive tumors (p = 0.008). Whereas RAGE was equally reduced in thymic hyperplasia and epithelial tumors (p = 0.003), HMGB1 was only elevated in malignancies (p = 0.036). Results were most pronounced in thymic carcinomas. Thus, RAGE and HMGB1 are involved in the (patho-)physiology of thymus, as evidenced by differentiated thymic and systemic expression patterns that may act as diagnostic or therapeutic targets in autoimmune disease and cancer.

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“…Immunohistochemical staining was performed both on paraffin and on frozen sections as previously described 21, using various monoclonal antibodies (see the above section entitled ‘Monoclonal antibodies’). Antigen retrieval was performed only for paraffin sections.…”

Section: Methodsmentioning

confidence: 99%

Expression of pentraxin 3 (PTX3) in human atherosclerotic lesions

Savchenko

Imamura

Ohashi

et al. 2008

The Journal of Pathology

184

158

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Pentraxin 3 (PTX3) and C-reactive protein (CRP) are members of the pentraxin superfamily. PTX3 expression is induced in response to inflammatory signals, and is produced at sites of inflammation by several types of cell, primarily monocytes/macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells (SMCs), and fibroblasts, but is not produced by hepatocytes, which are a major source of CRP. The aim of our study was to investigate the expression pattern of PTX3 in human atherosclerotic lesions using a novel monoclonal antibody against PTX3. We examined coronary arterial thrombi containing an atherosclerotic plaque component removed from patients with acute myocardial infarction and human aortic tissues with various degrees of atherosclerosis sampled from autopsy cases. Immunohistochemical study of paraffin and frozen sections indicated that macrophages, mainly foam cells, expressed PTX3 in advanced atherosclerotic lesions. Interestingly, we also clearly observed PTX3-positive neutrophils infiltrating into atherosclerotic plaques, suggesting that PTX3 derived from neutrophils as well as macrophages plays an important role in atherogenesis.

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Development and maturation of thymic dendritic cells during human ontogeny

Cited by 15 publications

References 19 publications

Ontogeny of Intrinsic Innervation in the Human Thymus and Spleen

Ontogeny of Intrinsic Innervation in the Human Thymus and Spleen

Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

Expression of pentraxin 3 (PTX3) in human atherosclerotic lesions

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