Development and mapping of microsatellites from a microdissected BTA 11‐specific DNA library

Abstract

A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.

Duplicated copies of the bovine JH locus contribute to the Ig repertoire

“…It is known that a duplication of the IgM locus exists on BTA11 (16). PCR was carried out with primers Cm forward and Cm reverse ( Table 1) using DNA from a lambda library prepared speci®cally from bovine chromosome 11 (17). This reaction was also carried out with BAC 944D11, a clone found to carry the J H locus isolated in our studies (see Results), and BAC 66R4C11, a clone carrying a second J H locus (18,19).…”

High-resolution genetic map of bovine chromosome 29 through focused marker development

“…The bovine chromosome 29 library was constructed by microdissection of a 1:29 Rb-fusion cell line as described in Ambady et al (1997Ambady et al ( , 2001). The Ï-ZAP library was plated at F5,000 pfu per 150-mm LB plate and grown overnight at 37°C.…”

“…Zhao et al (1999) recently reported 22 new markers from a microdissected library of porcine chr 6 (SSC6) and Wang et al (2000) isolated 31 markers from an SSC8 library. Recently, Ambady et al (2001) isolated 17 new ms markers from a BTA11-specific library and Liu et al (in press) obtained 34 new ms markers from a BTAY library. Here we report on the isolation and mapping of new markers from a bovine chromosome 29 (BTA29) library prepared by microdissection.…”