Development and Laboratory Testing of an Automated Monitor for the Measurement of Atmospheric Particle-Bound Reactive Oxygen Species (ROS)

Venkatachari, Prasanna; Hopke, Philip K.

doi:10.1080/02786820802227345

Cited by 71 publications

(96 citation statements)

References 22 publications

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“…Some preliminary results exist for an online method to measure ROS p (Venkatachari and Hopke, 2008;Wang et al, 2011). This instrument couples the particle-into-liquid sampler (PILS) with a flow system that mixes DCFH and a catalyst, peroxidase from horseradish (HRP) with the PILS sample stream-contained soluble PM 2.5 components (Venkatachari and Hopke, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…This instrument couples the particle-into-liquid sampler (PILS) with a flow system that mixes DCFH and a catalyst, peroxidase from horseradish (HRP) with the PILS sample stream-contained soluble PM 2.5 components (Venkatachari and Hopke, 2008). After utilizing mixing elements to combine and sufficiently react the sample ROS with the fluorescent reagents, the sample is measured using a spectrometer.…”

Section: Introductionmentioning

confidence: 99%

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Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay

King

Weber

2013

Atmos. Meas. Tech.

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Abstract. An online, semi-continuous instrument to measure fine particle (PM2.5) reactive oxygen species (ROS) was developed based on the fluorescent probe 2'7'-dichlorofluorescin (DCFH). Parameters that influence probe response were first characterized to develop an optimal method for use in a field instrument. The online method used a mist chamber scrubber to collect total (gas plus particle) ROS components (ROSt) alternating with gas phase ROS (ROSg) by means of an inline filter. Particle phase ROS (ROSp) was determined by the difference between ROSt and ROSg. The instrument was deployed in urban Atlanta, Georgia, USA, and at a rural site during various seasons. Concentrations from the online instrument generally agreed well with those from an intensive filter measurement of ROSp. Concentrations of the ROSp measurements made with this instrument were lower than reported in other studies, often below the instrument's average limit of detection (0.15 nmol H2O2 equivalents m−3). Mean ROSp concentrations were 0.26 nmol H2O2 equivalents m−3 at the Atlanta urban sites compared to 0.14 nmol H2O2 equivalents m−3 at the rural site.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay

King

Weber

2013

Atmos. Meas. Tech.

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“…Sameenoi et al 18 have produced a microfluidic method for DTT analysis, which may be widely utilized in future studies. Venkatachari and Hopke 19 compared the responses of DCFH-DA, POPHAA and DTT to measure ROS compounds in ambient air. King and Weber 20 developed a DCFH-based on-line method for the measurement of particulate, gaseous as well as total ROS.…”

Section: Ros Measurementmentioning

confidence: 99%

“…On-line methods for ROS determination have also been developed to understand their characteristics on diurnal basis [18][19][20] . Sameenoi et al 18 have produced a microfluidic method for DTT analysis, which may be widely utilized in future studies.…”

Section: Ros Measurementmentioning

confidence: 99%

Oxidative Potential of Ambient Aerosols:An Indian Perspective

Rastogi¹,

Patel²

2017

Current Science

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Keywords: Ambient aerosols, oxidative potential, reactive oxygen species, pollutants sources.AMBIENT aerosols are tiny particles suspended in the air with size predominantly ranging between 10 nm to 10 m. Aerosols consist of a wide variety of organic and inorganic chemical species such as major cations and anions, transition metals, heavy metals, elemental carbon (EC) and organic carbon species. They are injected into the atmosphere from a variety of natural (e.g. mineral dust, sea-salt, forest fire, volcanic eruption) and anthropogenic (e.g. biomass burning, fossil-fuel burning, vehicular and industrial emissions) sources, either directly (primary particles) or through the chemical processing of their gaseous precursors (secondary particles), or both. Aerosols are known to affect the earth's climate (radiation budget, hydrological cycle), aquatic ecosystem (biogeochemistry of oceans and lakes), and air quality (visibility and human health) 1 . Aerosols react with several atmospheric trace gases that leads to change in their chemical and physical properties, which can affect their optical and hygroscopic properties as well as bioavailability of nutrients. Although all the effects of aerosols are important, the effect on air quality is gaining specific interest and attention, as it directly relates to public health. There are numerous studies in the literature showing a direct relationship between high concentrations of ambient fine particles, and morbidity and mortality 2-6 . One of the widely proposed mechanisms related to the effect of aerosols on human health is that several types of aerosols produce reactive oxygen species (ROS) in situ in the human respiratory system, while breathing. Production of ROS causes imbalance between oxidants and antioxidants in the body and leads to several cardiopulmonary diseases like bronchitis, breathing problem, asthma, lung cancer, heart attack and even death 2-4 . ROS are oxygen-containing chemical species with strong oxidizing capability. Although molecular oxygen is not very reactive due to spin restriction in its outermost orbits, when paramagnetic centres (different aerosol species) react with O 2 , ROS are generated. They include families of oxygen-centred or related free radicals, ions and molecules. The free-radical family includes, but is not limited to, hydroxyl, hydroperoxyl and organic peroxy radicals, ions consist of superoxide, hypochlorite and peroxynitrite ions, and molecules are represented by hydrogen peroxide, organic and inorganic peroxides (Figure 1). These ROS can be exogenous (ROS are constituents of the particle) and/or endogenous (formed by in situ reactions where particles act as catalysts). Exogenous ROS are also called particle-bound ROS, whereas endogenous ROS are called particle-induced ROS. Here, it is important to note that ambient particles act as a catalyst for the generation of endogenous ROS and therefore, they can be active until they are removed from the place of generation (atmosphere, human respiratory system, plants). Reactivity of ...

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“…One such assay monitors the rapid decay of 60 2',7'-dichloorofluorescin (DCFH) to a fluorescent compound (DCF) in the presence of horseradish peroxidase (HRP) (King and Weber, 2013;Fuller et al, 2014b;Huang et al, 2016;Wang et al, 2011). The DCFH assay has been shown to be sensitive towards a broad range of ROS, and to have fast response rates and linear response to varying ROS concentrations, thus being suitable to evaluate the overall oxidative activity of PM (Zhou et al, 1997;Venkatachari and Hopke, 2008;King andWeber, 2013, Zhou et al, 2017). experiments were conducted.…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species (ROS) emissions and formation pathways in residential wood smoke under different combustion and aging conditions

Zhou¹,

Zotter²,

Bruns³

et al. 2017

Preprint

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Abstract. Wood combustion emissions can induce oxidative stress in the human respiratory tract caused by reactive 15 oxygen species (ROS), either directly or after oxidation in the atmosphere. To improve our understanding of the ROS generation potential of wood combustion emissions, a suite of smog chamber (SC) and potential aerosol mass (PAM) chamber experiments were conducted under well determined conditions for different combustion devices and technologies, different fuel types, operation methods, combustion regimes, combustion phases and aging conditions. The ROS content as well as the chemical properties of the aerosols were quantified by a novel ROS 20 analyzer and a high resolution time of flight aerosol mass spectrometer (HR-ToF-AMS). For all eight tested combustion devices, primary ROS concentrations substantially increased upon aging. The level of primary and aged ROS emission factors (EF ROS ) were dominated by the combustion device (within different combustion technologies) and to a greater extent by the combustion regimes: the variability within one device was much higher than the variability of EF ROS from different devices. Aged EF ROS under bad combustion conditions were ~2-80 times higher 25 than under optimum combustion conditions. EF ROS from automatically operated combustion devices were on average one order of magnitude lower than those from manually operated appliances, which indicates that automatic combustion devices operated at optimum conditions to achieve near-complete combustion should be employed to

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Development and Laboratory Testing of an Automated Monitor for the Measurement of Atmospheric Particle-Bound Reactive Oxygen Species (ROS)

Cited by 71 publications

References 22 publications

Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay

Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay

Oxidative Potential of Ambient Aerosols:An Indian Perspective

Reactive oxygen species (ROS) emissions and formation pathways in residential wood smoke under different combustion and aging conditions

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