Development and Investigation of HRM Markers to Discriminate Two Ogura Cytoplasmic Male Sterility Restorer Genes in Radish
Hong-Il Ahn,
Koeun Han,
Hee-Bum Yang
et al.
Abstract:Ogura male sterile cytoplasm is widely used for radish breeding. In this study, high-resolution melting (HRM) markers associated with Rft and Rfo, major restorer-of-fertility genes in Ogura cytoplasmic male sterility (CMS) in radish, were developed. Genetic mapping was carried out using F2 populations derived from crosses between male-sterile Ogura CMS lines and male-fertile lines. Identification of the Rft and Rfo loci was achieved through SNP-based genotyping and linkage grouping. HRM markers were subsequent… Show more
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