Development and interspecific transferability of genic microsatellite markers in Spartina spp. with different genome size

Baisakh, Niranjan; Subudhi, Prasanta K.; Arumuganathan, K.; Parco, Arnold; Harrison, Stephen A.; Knott, Carrie A.; Materne, Michael

doi:10.1016/j.aquabot.2009.07.007

Cited by 16 publications

(14 citation statements)

References 33 publications

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“…Thirteen molecular markers (Table 2; Blum et al, 2004; Baisakh et al, 2009), which generated 30 loci, were used to genotype LA11‐101, LA11‐102, LA11‐103, and Vermilion. Jaccard similarity coefficients (Jaccard, 1908) were calculated as a /( a + b + c ) where; a = the number of matching present bands (‘1’ and ‘1’), b = the number of unmatched bands (‘1’ and ‘0’), and c = the number of unmatched bands (‘0’ and ‘1’).…”

Section: Methodsmentioning

confidence: 99%

Registration of Smooth Cordgrass Cultivars ‘LA11‐101’, ‘LA11‐102’, and ‘LA11‐103’

Knott

Materne

Utomo

et al. 2012

J of Plant Registrations

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Smooth cordgrass (Spartina alterniflora Loisel.) is a perennial intertidal saline marsh grass used in restoration projects throughout the northern coast of the Gulf of Mexico and the Atlantic coast of the United States to mitigate coastal erosion. Three vigorous, genetically distinct cultivars with high seed set and germination and rapid recovery after transplant were developed from seeds collected from natural populations throughout Louisiana: ‘LA11‐101’ (Reg. No. CV‐261, PI 664963), ‘LA11‐102’ (Reg. No. CV‐262, PI 664964), and ‘LA11‐103’ (Reg. No. CV‐263, PI 664965). LA11‐101, LA11‐102, and LA11‐103 are clonal cultivars that were tested in replicated field trials for 9 yr in 11 environments. LA11‐101, LA11‐102, and LA11‐103 recovered more quickly after transplant and produced more viable seeds than ‘Vermilion’, the only smooth cordgrass cultivar adapted to Louisiana environments. The average plant vigor, plant spread, and number of stems per plot were similar for LA11‐101, LA11‐102, LA11‐103 and Vermilion. The genetic diversity of LA11‐101, LA11‐102, LA11‐103 and Vermilion was determined using 30 loci, and the Jaccard similarity coefficients of all four ranged from 0.083 to 0.733. LA11‐101, LA11‐102, and LA11‐103 are vigorous, genetically distinct, smooth cordgrass cultivars with superior seed set, seed germination, and transplant recovery and are recommended for saline marsh restoration projects in the northern Gulf of Mexico.

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Section: Methodsmentioning

confidence: 99%

Registration of Smooth Cordgrass Cultivars ‘LA11‐101’, ‘LA11‐102’, and ‘LA11‐103’

Knott

Materne

Utomo

et al. 2012

J of Plant Registrations

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“…Eleven microsatellite markers (Table 1; Blum et al, 2004; Baisakh et al, 2009) that generated 25 alleles were used to genotype St. Bernard, Las Palomas, Lafourche, Vermilion, and nine additional smooth cordgrass genotypes. Jaccard similarity coefficients (Jaccard, 1908) were calculated as a /( a + b + c ), where a = the number of matching present bands (‘1’ and ‘1’), b = the number of unmatched bands (‘1’ and ‘0’), and c = the number of unmatched bands (‘0’ and ‘1’).…”

Section: Methodsmentioning

confidence: 99%

Registration of ‘St. Bernard’, ‘Las Palomas’, and ‘Lafourche’ Smooth Cordgrass Cultivars

Knott

Materne

Utomo

et al. 2012

J of Plant Registrations

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Numerous wetland management and restoration projects utilize smooth cordgrass (Spartina alterniflora Loisel.). Unfortunately, only one released smooth cordgrass cultivar, 'Vermilion', is currently available for use in these projects in Louisiana. Three improved smooth cordgrass cultivars, 'St. Bernard' (LA12-101) (Reg. No. CV-268, PI 665014), 'Las Palomas' (LA12-102) (Reg. No. CV-269, PI 665015), and 'Lafourche' (LA12-103) (Reg. No. CV-270, PI 665016), were developed from seeds collected from natural populations in Louisiana and Texas. They are improved, genetically different smooth cordgrass clonal cultivars that were evaluated and compared with Vermilion in replicated field trials for 9 yr in 11 environments. St. Bernard, Las Palomas, and Lafourche recovered more quickly after transplant and produced more viable seeds than Vermilion. The average plant vigor, plant spread, and number of stems per plot for these cultivars were equal to those of Vermilion. DNA fingerprinting data, based on 11 polymorphic microsatellite markers, indicated that St. Bernard, Las Palomas, and Lafourche are genetically different from Vermilion. St. Bernard, Las Palomas, and Lafourche are vigorous, genetically distinct smooth cordgrass cultivars with superior seed set, seed germination, and transplant recovery and therefore are recommended for saline marsh restoration projects in the northern Gulf of Mexico.

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“…Both polyploidy and aneuploidy are observed in Spartina genus with no known diploid species. The basic chromosome number of the Spartina genus is x=10 and 2n=40-124 (Marchant 1968 (Baisakh et al 2009b).…”

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Spartina alterniflora Loisel., a halophyte grass model to dissect salt stress tolerance

Subudhi

Baisakh

2011

In Vitro Cell.Dev.Biol.-Plant

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Salinity is one of the most serious abiotic stresses affecting crop productivity worldwide. Improving tolerance to salinity in field crops is globally important because a majority of the world population relies on salt-sensitive crops such as rice, corn, and wheat for their daily calories. Although there is no salt stress sensor yet identified, different signaling components and tolerance mechanisms have been substantiated to a great extent in a glycophyte like Arabidopsis, and more recently in a few halophytes. With the rapid advances in genetics, genomics, and biochemical and transformation tools, it is now possible to explore the genetic and molecular basis of the unusually high level of salt tolerance in halophilic plants. We will focus on a halophyte grass, Spartina alterniflora, commonly known as smooth cordgrass, which possesses all known mechanisms of salt tolerance and subsequent exploitation of its genome information for crop improvement. A number of candidate genes encoding transcription factors, ion transport, osmoprotectants, antioxidants, detoxifying enzymes, etc. have been identified. Although recent efforts to develop salt tolerant cultivars that could retain the halophytic traits through transgenesis show some promise, further exploration is needed to test the contribution of single or multiple salt stress-related genes or regulatory factors from halophilic plants, including S. alterniflora, for possible utilization in crop improvement.

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Development and interspecific transferability of genic microsatellite markers in Spartina spp. with different genome size

Cited by 16 publications

References 33 publications

Registration of Smooth Cordgrass Cultivars ‘LA11‐101’, ‘LA11‐102’, and ‘LA11‐103’

Registration of Smooth Cordgrass Cultivars ‘LA11‐101’, ‘LA11‐102’, and ‘LA11‐103’

Registration of ‘St. Bernard’, ‘Las Palomas’, and ‘Lafourche’ Smooth Cordgrass Cultivars

Spartina alterniflora Loisel., a halophyte grass model to dissect salt stress tolerance

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