Development and initial application of a fully integrated photoacoustic micro-ultrasound system

Needles, A.; Heinmiller, Andrew; Sun, Jesse; Theodoropoulos, Catherine; Bates, DO; Hirson, D.; Yin, Melissa; Foster, F. Stuart

doi:10.1109/tuffc.2013.2646

Cited by 186 publications

(160 citation statements)

References 16 publications

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“…The resolution needed for detection of extremely low SO 2 levels could be challenging for nontomographic PA scanners. These challenges are especially dominant in higher depths, due to the wavelength-dependent absorption (19).…”

Section: Discussionmentioning

confidence: 99%

Functional Flow Patterns and Static Blood Pooling in Tumors Revealed by Combined Contrast-Enhanced Ultrasound and Photoacoustic Imaging

et al. 2016

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Alterations in tumor perfusion and microenvironment have been shown to be associated with aggressive cancer phenotypes, raising the need for noninvasive methods of tracking these changes. Dynamic contrast-enhanced ultrasound (DCEUS) and photoacoustic (PA) imaging serve as promising candidates-one has the ability to measure tissue perfusion, whereas the other can be used to monitor tissue oxygenation and hemoglobin concentration. In this study, we investigated the relationship between the different functional parameters measured with DCEUS and PA imaging, using two morphologically different hind-limb tumor models and drug-induced alterations in an orthotopic breast tumor model. Imaging results showed some correlation between perfusion and oxygen saturation maps and the ability to sensitively monitor antivascular treatment. In addition, DCEUS measurements revealed different vascular densities in the core of specific tumors compared with their rims. Noncorrelated perfusion and hemoglobin concentration measurements facilitated discrimination between blood lakes and necrotic areas. Taken together, our results illustrate the utility of a combined contrast-enhanced ultrasound method with photoacoustic imaging to visualize blood flow patterns in tumors.Cancer Res; 76(15); 4320-31. Ó2016 AACR.

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Section: Discussionmentioning

confidence: 99%

Functional Flow Patterns and Static Blood Pooling in Tumors Revealed by Combined Contrast-Enhanced Ultrasound and Photoacoustic Imaging

et al. 2016

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“…To achieve QPAE, we combined a linear-array-based photoacoustic tomography system (Vevo LAZR Imaging System, VisualSonics Inc., Toronto, Canada) with a customized compression system and improved the image reconstruction 19,20 [ Fig. 1].…”

Section: Experimental Systemmentioning

confidence: 99%

Quantitative photoacoustic elastography of Young’s modulus in humans

Hai

Zhou

Gong

et al. 2017

Photons Plus Ultrasound: Imaging and Sensing 2017

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Elastography can noninvasively map the elasticity distribution of biological tissue, which is often altered in pathological states. In this work, we report quantitative photoacoustic elastography (QPAE), capable of measuring Young's modulus of human tissue in vivo. By combining photoacoustic elastography with a stress sensor having known stress-strain behavior, QPAE can simultaneously measure strain and stress, from which Young's modulus is calculated. We first applied QPAE to quantify the Young's modulus of tissue-mimicking agar phantoms with different concentrations. The measured values fitted well with both the empirical expectations based on the agar concentrations and those measured in independent standard compression tests. We then demonstrated the feasibility of QPAE by measuring the Young's modulus of human skeletal muscle in vivo. The data showed a linear relationship between muscle stiffness and loading. The results proved that QPAE can noninvasively quantify the absolute elasticity of biological tissue, thus enabling longitudinal imaging of tissue elasticity. QPAE can be exploited for both preclinical biomechanics studies and clinical applications.

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“…To image melanoma CTC clusters, we applied an LA-PAT system based on a handheld probe and improved the image reconstruction 22,23 . A tunable optical parametric oscillator laser (680 to 970 nm, 20-Hz pulse repetition rate) was used for illumination.…”

Section: Experimental Systemmentioning

confidence: 99%

Linear-array-based photoacoustic tomography for label-free high-throughput detection and quantification of circulating melanoma tumor cell clusters

Hai

Zhou

Zhang

et al. 2017

Photons Plus Ultrasound: Imaging and Sensing 2017

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Circulating tumor cell (CTC) clusters arise from multicellular grouping in the primary tumor and elevate the metastatic potential by 23 to 50 fold compared to single CTCs. High throughout detection and quantification of CTC clusters is critical for understanding the tumor metastasis process and improving cancer therapy. In this work, we report a lineararray-based photoacoustic tomography (LA-PAT) system capable of label-free high-throughput CTC cluster detection and quantification in vivo. LA-PAT detects CTC clusters and quantifies the number of cells in them based on the contrast-to-noise ratios (CNRs) of photoacoustic signals. The feasibility of LA-PAT was first demonstrated by imaging CTC clusters ex vivo. LA-PAT detected CTC clusters in the blood-filled microtubes and computed the number of cells in the clusters. The size distribution of the CTC clusters measured by LA-PAT agreed well with that obtained by optical microscopy. We demonstrated the ability of LA-PAT to detect and quantify CTC clusters in vivo by imaging injected CTC clusters in rat tail veins. LA-PAT detected CTC clusters immediately after injection as well as when they were circulating in the rat bloodstreams. Similarly, the numbers of cells in the clusters were computed based on the CNRs of the photoacoustic signals. The data showed that larger CTC clusters disappear faster than the smaller ones. The results prove the potential of LA-PAT as a promising tool for both preclinical tumor metastasis studies and clinical cancer therapy evaluation.

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Development and initial application of a fully integrated photoacoustic micro-ultrasound system

Cited by 186 publications

References 16 publications

Functional Flow Patterns and Static Blood Pooling in Tumors Revealed by Combined Contrast-Enhanced Ultrasound and Photoacoustic Imaging

Functional Flow Patterns and Static Blood Pooling in Tumors Revealed by Combined Contrast-Enhanced Ultrasound and Photoacoustic Imaging

Quantitative photoacoustic elastography of Young’s modulus in humans

Linear-array-based photoacoustic tomography for label-free high-throughput detection and quantification of circulating melanoma tumor cell clusters

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