Development and<i>In Vitro</i>Characterization of Hyaluronic Acid-Based Coatings for Implant-Associated Local Drug Delivery Systems

Petersen, Svea; Kaule, Sebastian; Teske, Michael; Minrath, Ingo; Schmitz, Klaus‐Peter; Sternberg, Katrin

doi:10.1155/2013/587875

Cited by 14 publications

(16 citation statements)

References 30 publications

(33 reference statements)

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“…This seems essential for reduction of PTX wash off as we observed in a previous study that the presence of free PTX crystals above the coating matrix resulted in high drug wash off rates [27]. As we contrarily assume from our own and foreign previous investigations, that crosslinking density has an impact on drug release [28,29], resulting performance of photochemically crosslinked PVP coatings on DCB was again evaluated with regard to PTX wash off and transfer upon balloon expansion in a silicone tube as vessel model in dependence of UV radiation time. Figure 4 evidences observed tendencies, which were decreasing PTX wash off rates with elevated radiation time and initially increased PTX transfers upon balloon expansion in a silicone tube, with a maximum at 10 min radiation, followed by decay to the base level.…”

Section: Development and In Vitro Characterization Of Pvp-coated Dcb supporting

confidence: 50%

Development and in Vitro Characterization of Photochemically Crosslinked Polyvinylpyrrolidone Coatings for Drug-Coated Balloons

Petersen¹,

Minrath²,

Kaule³

et al. 2013

Coatings

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Polyvinylpyrrolidone (PVP) is a conventionally applied hydrophilic lubricious coating on catheter-based cardiovascular devices, used in order to ease movement through the vasculature. Its use as drug reservoir and transfer agent on drug-coated balloons (DCB) is therefore extremely promising with regard to the simplification of its approval as a medical device. Here, we developed a PVP-based coating for DCB, containing paclitaxel (PTX) as a model drug, and studied the impact of crosslinking via UV radiation on drug stability, wash off, and transfer during simulated use in an in vitro vessel model. We showed that crosslinking was essential for coating stability and needed to be performed prior to PTX incorporation due to decreased drug bioavailability as a result of photodecomposition and/or involvement in vinylic polymerization with PVP under UV radiation. Moreover, the crosslinking time needed to be carefully controlled. While short radiation times did not provide enough coating stability, associated with high wash off rates during DCB insertion, long radiation times lowered drug transfer efficiency upon balloon expansion. A ten minutes radiation of PVP, however, combined a minimized drug wash off rate of 34% with an OPEN ACCESSCoatings 2013, 3 254 efficient drug transfer of 49%, underlining the high potential of photochemically crosslinked PVP as a coating matrix for DCB.

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Section: Development and In Vitro Characterization Of Pvp-coated Dcb supporting

confidence: 50%

Development and in Vitro Characterization of Photochemically Crosslinked Polyvinylpyrrolidone Coatings for Drug-Coated Balloons

Petersen¹,

Minrath²,

Kaule³

et al. 2013

Coatings

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“…To reduce the drug loss during advancing the DCB to the final lesion and to improve drug transfer to the vessel wall, new drug coatings based on cetylpyridinium salycate (Cetpyrsal) or hyaluronic acid (HA) have been developed and tested in an in-vitro track model [5,6]. This model is limited in a way that artificial materials like Teflon or silicone are used to mimic the arterial walls in-vitro.…”

Section: Introductionmentioning

confidence: 99%

“…This study describes a method to gain information about the in-vivo drug transfer in a simple porcine in-vitro model, comparing a commercial available DCB with two alternative coatings developed by Petersen et al [5,6]. in Cetpyrsal.…”

Section: Introductionmentioning

confidence: 99%

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Acute drug transfer and particle release of drug coated balloons within a porcine in-vitro model

Brandt-Wunderlich

Almstädt

Kaule

et al. 2017

Current Directions in Biomedical Engineering

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Drug coated balloons (DCB) are used in the therapy of coronary as well as peripheral artery disease. The success of drug transfer to the vessel wall depends on the excipient used in combination with paclitaxel as antiproliferative drug. Although in-vivo studies show very good results with this technology, there is a lack of in-vitro test methods for characterization of various DCB available on the market. This study describes a method to gain information about the drug transfer and the particle release of three different DCB based on cetylpyridinium salycate (Cetpyrsal), hyaluronic acid and iopromide within a porcine in-vitro model. The Cetpyrsal-based DCB showed promising results with the highest drug transfer while producing the lowest number of particles.

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“…HA is a beneficial structural component and basic building block of such HA-based carriers and biomaterials as HA-nanoparticles, hydrogels and scaffolds, and thus it offers great promise for diagnostics and even for therapy [10]. As already proven, HA also serves as an effective stabilizer for surface treatment and coating of a variety of medical devices and materials, including the likes of catheters [16], guidewires [17], superparamagnetic nanoparticles for in vivo imaging [18,19], various types of artificial replacements such as dental, orthopaedic implants [20,21] or neuronal implants [22], and microfluidic channels [23].…”

Section: Introductionmentioning

confidence: 99%

Magnetic microparticles post-synthetically coated by hyaluronic acid as an enhanced carrier for microfluidic bioanalysis

Holubová

Knotek

Palarčík

et al. 2014

Materials Science and Engineering: C

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Iron oxide based particles functionalized by bioactive molecules have been utilized extensively in biotechnology and biomedicine. Despite their already proven advantages, instability under changing reaction conditions, non-specific sorption of biomolecules on the particles' surfaces, and iron oxide leakage from the naked particles can greatly limit their application. As confirmed many times, surface treatment with an appropriate stabilizer helps to minimize these disadvantages. In this work, we describe enhanced post-synthetic surface modification of superparamagnetic microparticles varying in materials and size using hyaluronic acid (HA) in various chain lengths. Scanning electron microscopy, atomic force microscopy, phase analysis light scattering and laser diffraction are the methods used for characterization of HA-coated particles. The zeta potential and thickness of HA-layer of HA-coated Dynabeads M270 Amine were -50 mV and 85 nm, respectively, and of HA-coated p(GMA-MOEAA)-NH2 were -38 mV and 140 nm, respectively. The electrochemical analysis confirmed the zero leakage of magnetic material and no reactivity of particles with hydrogen peroxide. The rate of non-specific sorption of bovine serum albumin was reduced up to 50% of naked ones. The coating efficiency and suitability of biopolymer-based microparticles for magnetically active microfluidic devices was confirmed. Highlights: Post-synthetic surface modification of magnetic microparticles by hyaluronic acid  Hyaluronic acid -polymer of unique physicochemical and biological characteristics ABSTRACTIron oxide based particles functionalized by bioactive molecules have been utilized extensively in biotechnology and biomedicine. Despite their already proven advantages, instability under changing reaction conditions, non-specific sorption of biomolecules on the particles' surfaces, and iron oxide leakage from the naked particles can greatly limit their application. As confirmed many times, surface treatment with an appropriate stabilizer helps to minimize these disadvantages.In this work, we describe enhanced post-synthetic surface modification of superparamagnetic microparticles varying in materials and size using hyaluronic acid (HA) in various chain lengths. Scanning electron microscopy, atomic force microscopy, phase analysis light scattering and laser diffraction are the methods used for characterization of HA-coated particles. The zeta potential and thickness of HA-layer of HA-coated Dynabeads M270Amine were -50 mV and 85 nm, respectively, and of HA-coated p(GMA-MOEAA)-NH 2 were -38 mV and 140 nm, respectively. The electrochemical analysis confirmed the zero leakage of magnetic material and no reactivity of particles with hydrogen peroxide. The rate of nonspecific sorption of bovine serum albumin was reduced up to 50% of naked ones. The coating efficiency and suitability of biopolymer-based microparticles for magnetically active microfluidic devices was confirmed.

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Development andIn VitroCharacterization of Hyaluronic Acid-Based Coatings for Implant-Associated Local Drug Delivery Systems

Cited by 14 publications

References 30 publications

Development and in Vitro Characterization of Photochemically Crosslinked Polyvinylpyrrolidone Coatings for Drug-Coated Balloons

Development and in Vitro Characterization of Photochemically Crosslinked Polyvinylpyrrolidone Coatings for Drug-Coated Balloons

Acute drug transfer and particle release of drug coated balloons within a porcine in-vitro model

Magnetic microparticles post-synthetically coated by hyaluronic acid as an enhanced carrier for microfluidic bioanalysis

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