Development and forensic applications of multiplex PCR of autosomal biallele polymorphisms

Turchi, Chiara; Pesaresi, Mauro; Presciuttini, Silvano; Alessandrini, Federica; Sassaroli, Corrado; Tagliabracci, Adriano

doi:10.1016/s0531-5131(03)01530-9

Cited by 2 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The discrimination power of STRs is about 4 times higher than SNPs, so in forensic cases we need more or less 60 SNP markers to reach the discrimination power of a routine used multiplex STR analysis kit with 15 STR markers [12,13,16,24,25,27,43,44]. In this study we determined only 18 polymorphic Y-SNP markers for Antalya population.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Y-SNP haplogroups in the Antalya population in Turkish Republic

Serdar¹,

Demirçin

2009

Rom J Leg Med

View full text Add to dashboard Cite

SNPs are known to be the most abundant source of sequence variation in the human genome. The SNPs in the NRY (non-recombining Y-chromosome) region which passes from father to son as unchanged haplotype-blocks escaping recombination, provides important advantages in the investigations of sexual assault crimes, in the cases of parentage testing especially if the mother or alleged father is unavailable for testing and in the evolutionary studies. The aim of this study, was to determine the frequencies of Y SNP markers and the haplogroups, in order to define the Y-chromosome SNP markers which are polymorphic, have high discrimination power and can be used in forensic investigations in the Antalya population. For each of 75 unrelated males from Antalya, 35 different Y-SNP markers were amplified in a single reaction using multiplex minisequencing method. In the study, 18 markers of them were found to be polymorphic. The most frequent Y-SNP markers with mutations were M139 (100%), SRY 10831 /SRY 1532 (92%), M89 (85.3%), M213 (85.3%), M9 (44%), 92R7 (30.6%), 12F2 (30.6%), M45 (29.3%), M172 (26.6%) and M173 (22.6%). The Y-chromosome haplogroups of Antalya population were defined by these 18 Y-SNP polymorphic loci and the frequencies and the distribution of haplogroups were determined. J2*(xJ2F2) (26.6%), K*(xN3,O,P) (13.3%), E3b (9.3%), F*(xH,I,J,K) (8%), R1a1*(xR1a1b) (8%), R1b*(xR1b1, R1b6, R1b8) (8%), P*(xQ3a,R1) (8%) haplogroups were identified as the most abundant in Antalya population. These haplogroups are reported as widespread also in European and neighboring Near Eastern populations.

show abstract

Section: Discussionmentioning

confidence: 99%

“…SNPs can be analysed in short amplicons and this advantage makes it possible to analyse heavily degraded biological samples which can usualy be big problems in forensic investigations [12][13][14][15][16][17]19,21,[24][25][26][27][28][29][30].…”

mentioning

confidence: 99%

Y-SNP haplogroups in the Antalya population in Turkish Republic

Serdar¹,

Demirçin

2009

Rom J Leg Med

View full text Add to dashboard Cite

show abstract

SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues.

Bąbol-Pokora

Berent

2008

Acta Biochim Pol

View full text Add to dashboard Cite

SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.

show abstract

Development and forensic applications of multiplex PCR of autosomal biallele polymorphisms

Cited by 2 publications

References 4 publications

Y-SNP haplogroups in the Antalya population in Turkish Republic

Y-SNP haplogroups in the Antalya population in Turkish Republic

SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues.

Contact Info

Product

Resources

About