Development and evaluation of silver amplification immunochromatography kit for foot-and-mouth disease virus antigen detection

Morioka, Kazuki; Urayama, Kana; Wada, Atsuhiko; Yoshida, Kazuo; Kato, Tomoko; Kitano, Rie; Nishi, Tatsuya; Kanno, Toru; Yamada, Manabu; Yamakawa, Makoto; Ulziibat, Gelermaa; Makino, Yoshihiko; Nakamura, Kentaro; Hojo, Eri; Matsumoto, Takashi; Fukai, Katsuhiko

doi:10.1016/j.jviromet.2019.113736

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…26 Output signal-amplification techniques augment the performance of LFIAs by increasing their sensitivity. 39,40 Our antigen-detection assays can be expected to perform as a clinical diagnostic tool. By detecting cultured SARS-CoV-2 particles as low as 6.3 3 10 4 copies/mL, our LFIA was more sensitive than other commercially available antigen-detection kits.…”

Section: Discussionmentioning

confidence: 99%

Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests

Yamaoka

Miyakawa

Jeremiah

et al. 2021

Cell Reports Medicine

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The ongoing COVID-19 pandemic is a major global public health concern. Although rapid point-of-care testing for detecting viral antigen is important for management of the outbreak, the current antigen tests are less sensitive than nucleic acid testing. In our current study, we produce monoclonal antibodies (mAb) that exclusively react with SARS-CoV-2 and exhibit no cross-reactivity with other human coronaviruses including SARS-CoV. Molecular modeling suggest that the mAbs bind to epitopes present on the exterior surface of the nucleocapsid, making them suitable for detecting SARS-CoV-2 in clinical samples. We further select the optimal pair of anti-SARS-CoV-2 NP mAbs using ELISA, and then use this mAb pair to develop immunochromatographic assay augmented with silver amplification technology. Our mAbs recognize the variants of concern (501Y.V1-V3) that are currently in circulation. Due to their high performance, the mAbs of this study can serve as good candidates for developing antigen detection kits for COVID-19.

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Section: Discussionmentioning

confidence: 99%

Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests

Yamaoka

Miyakawa

Jeremiah

et al. 2021

Cell Reports Medicine

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“…The safety and efficiency of DHG have been tested and verified by proven historical facts, but gelatins made from other animal skins may have unpredictable and serious consequences [2][3][4]. For example, it has been indicated that cattle-hide gelatins (CHGs) may cause safety problems because of the risk of bovine spongiform encephalopathy and foot-and-mouth disease [5,6]. Pig-hide gelatins (PHGs) may present religious conflicts since the use of porcine foods and drugs is forbidden for people in some Islamic countries [7].…”

Section: Introductionmentioning

confidence: 99%

Species-specific identification of donkey-hide gelatin and its adulterants using marker peptides

Zhang

et al. 2022

PLoS ONE

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Donkey-hide gelatin is an important traditional Chinese medicine made from donkey skin. Despite decades of effort, identifying the animal materials (donkeys, horses, cattle and pigs) in donkey-hide gelatin remains challenging. In our study, we aimed to identify marker peptides of donkey-hide gelatin and its adulterants and develop a liquid chromatography–tandem mass spectrometry method to identify them. Theoretical marker peptides of four animals (donkeys, horses, cattle and pigs) were predicted and verified by proteomic experiments, and 12 species-specific marker peptides from donkey-hide gelatin and its adulterants were identified. One marker peptide for each gelatin was selected to develop the liquid chromatography–tandem mass spectrometry method. The applicability of the method was evaluated by investigating homemade mixed gelatin samples and commercial donkey-hide gelatin products. Using the liquid chromatography–tandem mass spectrometry method, the addition of cattle-hide gelatin and pig-hide gelatin to donkey-hide gelatin could be detected at a level of 0.1%. Horse-hide gelatin was detected when added at a level of 0.5%. Among 18 batches of donkey-hide gelatin products, nine were identified as authentic, and eight of the remaining samples were suspected to be adulterated with horse materials. These results provide both a practical method to control the quality of donkey-hide gelatin and a good reference for quality evaluations of other medicinal materials and foods containing protein components.

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Development and evaluation of silver amplification immunochromatography kit for foot-and-mouth disease virus antigen detection

Cited by 2 publications

References 17 publications

Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests

Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests

Species-specific identification of donkey-hide gelatin and its adulterants using marker peptides

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