Development and evaluation of RT-qPCR assays for two neglected orthobunyaviruses: Oya virus and Ebinur Lake virus

Liu, Siyuan; Chen, Wei; Nyaruaba, Raphael; Wang, Shunlong; Yang, Cihan; Wu, Qun; Liu, Ying; Liu, Puyu; Wang, Fei; Wang, Jingling; Yuan, Zhiming; Sun, Dingwei; Xia, Han

doi:10.1016/j.virusres.2023.199265

Cited by 2 publications

(3 citation statements)

References 28 publications

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“…For OYAV, the Luna® Universal Probe One-Step RT-qPCR Kit (NEB, Ipswich, USA) was used, and for EBIV, the HiScript II One Step qRT-PCR Probe Kit (Vazyme, Nanjing, China) was used. The primer and probe sets used are presented in previous studies [ 19 ]. The cutoff for positive samples determined via RT-qPCR was Ct (OYAV) < 36 and Ct (EBIV) < 35.…”

Section: Methodsmentioning

confidence: 99%

“…The OYAV was initially isolated from a pig suspected to be infected with the Nipah virus in Malaysia in 2000 [ 13 ]. Subsequent studies have detected or isolated OYAV from invertebrate or vertebrate hosts in China, Vietnam, and India [ 14 – 19 ]. Ebinur Lake virus, a member of the Bunyamwera serogroup, was initially isolated from the pools of Culex modestus in Xinjiang Province, China, and was later detected in various samples from Russia (mosquitoes, ticks, and wild birds) and Kenya ( Dasymys incomtus and Herpestes ichneumon ) [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the mosquito vector range for two orthobunyaviruses: Oya virus and Ebinur Lake virus

Liu,

Wang,

Wang

et al. 2024

Parasites Vectors

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Background Mosquito-borne viruses cause various infectious diseases in humans and animals. Oya virus (OYAV) and Ebinur Lake virus (EBIV), belonging to the genus Orthobunyavirus within the family Peribunyaviridae, are recognized as neglected viruses with the potential to pose threats to animal or public health. The evaluation of vector competence is essential for predicting the arbovirus transmission risk. Methods To investigate the range of mosquito vectors for OYAV (strain SZC50) and EBIV (strain Cu20-XJ), the susceptibility of four mosquito species (Culex pipiens pallens, Cx. quinquefasciatus, Aedes albopictus, and Ae. aegypti) was measured through artificial oral infection. Then, mosquito species with a high infection rate (IR) were chosen to further evaluate the dissemination rate (DR), transmission rate (TR), and transmission efficiency. The viral RNA in each mosquito sample was determined by RT-qPCR. Results The results revealed that for OYAV, Cx. pipiens pallens had the highest IR (up to 40.0%) among the four species, but the DR and TR were 4.8% and 0.0%, respectively. For EBIV, Cx. pipiens pallens and Cx. quinquefasciatus had higher IR compared to Ae. albopictus (1.7%). However, the EBIV RNA and infectious virus were detected in Cx. pipiens pallens, with a TR of up to 15.4% and a transmission efficiency of 3.3%. Conclusions The findings indicate that Cx. pipiens pallens was susceptible to OYAV but had an extremely low risk of transmitting the virus. Culex pipiens pallens and Cx. quinquefasciatus were susceptible to EBIV, and Cx. pipiens pallens had a higher transmission risk to EBIV than Cx. quinquefasciatus. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluating the mosquito vector range for two orthobunyaviruses: Oya virus and Ebinur Lake virus

Liu,

Wang,

Wang

et al. 2024

Parasites Vectors

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“…Ebinur Lake virus (EBIV) is a newly classified Orthobunyavirus first isolated from pools containing Culex modestus mosquitos in Xinjiang province, China [ 10 , 11 ]. Later, EBIV was detected in Culex mosquitos, ticks, rats, mongooses, and wild birds collected from Kenya and Russia [ 12 , 13 ]. Our previous work demonstrated that EBIV can infect various cell lines [ 14 ], cause acute lethal disease in adult BALB/c mice [ 15 ], and spread to Aedes aegypti mosquito saliva glands upon blood feeding [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Favipiravir Treatment Prolongs Survival in a Lethal BALB/c Mouse Model of Ebinur Lake Virus Infection

Geng,

Ren,

Yang

et al. 2024

Viruses

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Orthobunyavirus is the largest and most diverse genus in the family Peribunyaviridae. Orthobunyaviruses are widely distributed globally and pose threats to human and animal health. Ebinur Lake virus (EBIV) is a newly classified Orthobunyavirus detected in China, Russia, and Kenya. This study explored the antiviral effects of two broad-spectrum antiviral drugs, favipiravir and ribavirin, in a BALB/c mouse model. Favipiravir significantly improved the clinical symptoms of infected mice, reduced viral titer and RNA copies in serum, and extended overall survival. The median survival times of mice in the vehicle- and favipiravir-treated groups were 5 and 7 days, respectively. Favipiravir significantly reduced virus titers 10- to 100-fold in sera at all three time points compared to vehicle-treated mice. And favipiravir treatment effectively reduced the virus copies by approximately 10-fold across the three time points, relative to vehicle-treated mice. The findings expand the antiviral spectrum of favipiravir for orthobunyaviruses in vivo.

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Development and evaluation of RT-qPCR assays for two neglected orthobunyaviruses: Oya virus and Ebinur Lake virus

Cited by 2 publications

References 28 publications

Evaluating the mosquito vector range for two orthobunyaviruses: Oya virus and Ebinur Lake virus

Evaluating the mosquito vector range for two orthobunyaviruses: Oya virus and Ebinur Lake virus

Favipiravir Treatment Prolongs Survival in a Lethal BALB/c Mouse Model of Ebinur Lake Virus Infection

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