Development and evaluation of probe based real time loop mediated isothermal amplification for Salmonella: A new tool for DNA quantification

Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay

“…These characteristics provide results in a fast, efficient, and cost-effective method that theoretically should amplify target sequences with higher specificity than PCR. Since its introduction, LAMP has seen wide interdisciplinary application, for example in detecting microorganisms (e.g., bacteria23, fungi4, and viruses56) diagnosing genetic diseases7, and even early sex determination8.…”

Development of probe‐based real‐time loop‐mediated isothermal amplification for detection ofBrucella

“…Developed RT‐LAMP is a fast diagnostic tool for early detection and identification of microbial diseases (Mashooq et al . ). There was a significant decrease in amplification in the absence of probe.…”

“…; Mashooq et al . ). The positive samples were visualized by turbidity and colour detection with the addition of DNA‐intercalating dyes like SYBR green I dye.…”

“…; Mashooq et al . ). Its principal is similar to cascade rolling circle amplification, and is based on the auto cycling strand displacement DNA synthesis and emittance of fluorophore from the probe (Tanner et al .…”

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms

“…LAMP assay was monitored for the white precipitation caused by the presence of magnesium pyrophosphate, Mg 2 P 2 O 7 (a by-product of LAMP reaction) at optical density 650 nm every 6 s. Several studies have been reported using turbidity to detect the presence of desired micro-organisms, for instances, Mycoplasma pneumoniae (Sakai et al 2017), Salmonella spp. (Mashooq et al 2016), human papillomavirus (Kumvongpin et al 2016) and Clostridium difficile (Lin et al 2015). In case of unavailability of turbidimeter, a brief centrifugation of the LAMP products could be used as an alternative to observe the white precipitation, which is visible to naked eyes .…”