Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma

Tighsazzadeh, Mohammad; Mitchell, John C.; Boateng, Joshua

doi:10.1016/j.ijpharm.2019.05.059

Cited by 54 publications

(26 citation statements)

References 46 publications

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“…42,43 Furthermore, during the application of dressing for wound healing, the transparency of the material is crucial for the visual observations of the wound healing process. 44 The results of the visual observation of the inner and outer sides of each extracted membrane can be seen in Figure 4(a). The sample text can be clearly seen in the visual images from the inner side samples (LESM-strip, LESM-A0.5 and LESM-E0.9).…”

Section: Transparencymentioning

confidence: 99%

The eggshell membrane: A potential biomaterial for corneal wound healing

Mensah

Kim

et al. 2021

J Biomater Appl

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The eggshell membrane (ESM) is an abundant resource with innate complex structure and composition provided by nature. With at least 60 million tonnes of hen eggs produced globally per annum, utilisation of this waste resource is highly attractive in positively impacting sustainability worldwide. Given the morphology and mechanical properties of this membrane, it has great potential as a biomaterials for wound dressing. However, to date, no studies have demonstrated nor reported this application. As such, the objective of this investigation was to identify and optimise a reproducible extraction protocol of the ESM and to assess the physical, chemical, mechanical and biological properties of the substrate with a view to use as a wound dressing. ESM samples were isolated by either manual peeling (ESM-strip) or via extraction using acetic acid [ESM-A0.5] or ethylenediaminetetraacetic acid, EDTA [ESM-E0.9]. Energy dispersive X-ray spectroscopy (EDS) confirmed that there were no traces of calcium residues from the extraction process. Fourier transform infrared (FTIR) spectroscopy revealed that the extraction method (acetic acid and EDTA) did not alter the chemical structures of the ESM and also clarified the composition of the fibrous proteins of the ESM. Scanning electron microscopy (SEM) analyses revealed a three-layer composite structure of the ESM: an inner layer as continuous, dense and non-fibrous (limiting membrane), a middle layer with a network of fibres (inner shell membrane) and the outer layer (outer shell membrane) of larger fibres. Material properties including optical transparency, porosity, fluid absorption/uptake, thermal stability, mechanical profiling of the ESM samples were performed and demonstrated suitable profiles for translational applications. Biological in vitro studies using SV40 immortalised corneal epithelial cells (ihCEC) and corneal mesenchymal stromal cells (C-MSC) demonstrated excellent biocompatibility. Taken together, these results document the development of a novel sustainable biomaterial that may be used for ophthalmic wounds and/or other biomedical therapies.

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Section: Transparencymentioning

confidence: 99%

The eggshell membrane: A potential biomaterial for corneal wound healing

Mensah

Kim

et al. 2021

J Biomater Appl

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“…The ability of hydrophilic matrix systems to control the delivery of all types of drugs have been extensively investigated [ 52 , 53 ]. However, to the best of our knowledge, there is no previously reported release assay with Soluplus-based films.…”

Section: Resultsmentioning

confidence: 99%

“…This parameter was in the range of 0.5 < n < 1.0 which suggests that the release of CPX from all formulations followed a non-Fickian diffusion mechanism. The ability of hydrophilic matrix systems to control the delivery of all types of drugs have been extensively investigated [52,53]. However, to the best of our knowledge, there is no previously reported release assay with Soluplusbased films.…”

Section: In Vitro Cpx Release Form Filmsmentioning

confidence: 99%

Ciprofloxacin self-dissolvable Soluplus based polymeric films: a novel proposal to improve the management of eye infections

Guillot

Petalas

Skondra

et al. 2021

Drug Deliv. and Transl. Res.

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Infections of the eye are among the leading causes of vision impairment and vision loss worldwide. The ability of a drug to access the anterior parts of the eye is negligible after systemic administration. Effective drug delivery to the eye is a major challenge due to the presence of protective mechanisms and physiological barriers that result in low ocular availability after topical application. The main purpose of this work was the improvement of the corneal and conjunctival permeation of the antibiotic Ciprofloxacin, a wide spectrum antibiotic used for the most common eye infection, using a self-dissolving polymeric film. Films were prepared by the solvent casting technique, using polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft co-polymer (Soluplus), polyvynyl alcohol, and propylene glycol. Films were homogeneous in drug content and thickness, as demonstrated by adapting the Swiss Roll technique followed by microscopy observation. These films proved in vitro to control the release of the Ciprofloxacin. Ex vivo permeability studies using Franz diffusion cells and porcine cornea and sclera showed an effective permeability of the drug without inducing irritation of the tissues. Films swelled in contact with artificial tears forming an in situ gel over 20 min, which will improve drug contact and reduce the need of multiple dosing. The antibiotic activity was also tested in vitro in five types of bacterial cultures, assuring the pharmacological efficacy of the films. The developed films are a promising drug delivery system to topically treat or prevent ocular infections. Graphical abstract

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“…Films FA and FD were devoid of HPMC. Incorporation of HPMC into the formulations (FB, FC and FE) caused a significant (p<0.05) increase in the tensile strength and lowered the percentage of elongation at break (table 1), allowing the films formed by these formulations to be hard and tough [31].…”

Section: Physical-mechanical Propertiesmentioning

confidence: 99%

In-Situ Film-Forming Solution for Topical Application of Terbinafine Hcl: Biopharmaceutical Evaluation and in Vivo Antifungal Performance Using Animal Model

et al. 2021

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Objective: The main purpose of this study is to develop a film-forming solution with optimum physical-mechanical characteristics and excellent antifungal activity to enhance deposition and penetration into the stratum corneum (SC). Methods: The film-forming solutions of terbinafine HCl were formulated using methacrylate copolymers, polyethylene glycol 400, and ethanol as diluent. The selected formulations were subjected to test of physical-mechanical properties, drug release, drug permeation across the stratum corneum and drug deposition study. The best formulation was further evaluated for in vivo antifungal efficacy. Results: The selected formulations exhibited superior pharmaceutical characteristics, including rapid drying, non-stickiness, and being transparency on the skin. Formulation A (FA) had significantly lower tensile strength (4.78 N/m2, p<0.05) and higher percentage elongation at break (33.61%, p<0.05), which reduced the firmness of the film, allowing it to be super-flexible in following the movement of the skin and preventing loss of film through abrasion. FA showed significantly (p<0.05) rapid drug permeation (1510.51 µg/cm2) across the stratum corneum (SC) at 24 h when compared with the other formulations and the positive control proprietary drug (PD), Terbex® cream formulation (475.8 µg/cm2). Conclusion: Having superior physical-mechanical and drug permeation characteristics, FA can be considered as an efficient, reproducible, and efficacious antifungal formulation for topical application.

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Development and evaluation of performance characteristics of timolol-loaded composite ocular films as potential delivery platforms for treatment of glaucoma

Cited by 54 publications

References 46 publications

The eggshell membrane: A potential biomaterial for corneal wound healing

The eggshell membrane: A potential biomaterial for corneal wound healing

Ciprofloxacin self-dissolvable Soluplus based polymeric films: a novel proposal to improve the management of eye infections

In-Situ Film-Forming Solution for Topical Application of Terbinafine Hcl: Biopharmaceutical Evaluation and in Vivo Antifungal Performance Using Animal Model

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