Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice

Khanna, Apurva; Sharma, Vinay; Ellur, Ranjith Kumar; Shikari, Asif B.; Krishnan, S. Gopala; Singh, U. D.; Prakash, Ganesan; Sharma, Tilak Raj; Rathour, Rajeev; Variar, M.; Prashanthi, S. K.; Muthialu, Nagarajan; Vinod, K. K.; Bhowmick, Prolay Kumar; Singh, Nagendra; Prabhu, K. V.; Singh, B. D.; Singh, Ashok Kumar

doi:10.1007/s00122-015-2502-4

Cited by 105 publications

(122 citation statements)

References 21 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…The background selection using 50K chip was found to be highly informative as compared to low-density SSR markers as it provided better insight of the donor segments still remaining in the advanced backcross lines. Also, it is much more cost-effective (US$ 84/genotype with 50,051 SNP data points) as against the SSR markers (US$ 70/genotype with approximately 85-110 data points) [55] and therefore, use of SNP chip would be a better choice for the background selection in MABB.…”

Section: Background Selection Of Advanced Backcross Lines Using 50k Smentioning

confidence: 99%

From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network

Singh¹,

Singh²,

Xalaxo³

et al. 2016

Plant Science

Self Cite

192

101

View full text Add to dashboard Cite

Section: Background Selection Of Advanced Backcross Lines Using 50k Smentioning

confidence: 99%

From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network

Singh¹,

Singh²,

Xalaxo³

et al. 2016

Plant Science

Self Cite

192

101

View full text Add to dashboard Cite

“…The rice blast R gene Pi9 in the indica rice line 75-1-127 was introgressed from the wild species Oryza minuta (Amante-Bordeos et al 1992; Liu et al 2002) . The Pi9 gene conferred broad-spectrum disease resistance to 43 M. oryzae isolates collected from 13 countries (Liu et al 2002) and was used in rice breeding program (Khanna et al 2015; Koide et al 2011; Luo and Yin 2013; Ni et al 2015). …”

Section: Introductionmentioning

confidence: 99%

Marker-assisted breeding of the rice restorer line Wanhui 6725 for disease resistance, submergence tolerance and aromatic fragrance

Luo

Zhang

et al. 2016

Rice

View full text Add to dashboard Cite

BackgroundRice is a staple food crop in the world. With the increase in world population and economic development, farmers need to produce more rice in limited field. However, the rice production is frequently affected by biotic and abiotic stresses. The use of natural disease resistance and stress tolerance through genetic breeding is the most efficient and economical way to combat or acclimate to these stresses. In addition, rice with aromatic fragrance can significantly increase market value for its good grain quality. Mianhui 725 (MH725) is an elite restorer line that has been widely used to produce three-line hybrid rice in China. We previously introduced rice bacterial blight resistance genes Xa4 and Xa21 into MH725 and obtained an introgression rice line Wanhui 421 (WH421), which theoretically possesses 96.9% genetic background of MH725.ResultsHere we report the introduction and pyramiding of disease resistance genes Xa27 and Pi9, submergence tolerance gene Sub1A and aromatic fragrance gene badh2.1 in WH421 through backcrossing and marker-assisted selection. The newly developed introgression rice line was designated as Wanhui 6725 (WH6725), which theoretically possesses 95.0% genetic background of MH725. WH6725 and its hybrid rice conferred disease resistance to both blast and bacterial blight diseases and showed tolerance to submergence for over 14 days without significant loss of viability. Compared with non-aromatic rice MH725, WH6725 has strong aromatic fragrance. The major important agronomic traits and grain quality of WH6725 and its hybrid rice obtained in field trials were similar to those of MH725 and the control hybrid rice, indicating that WH6725 is as good as MH725 when it is used as a restorer line for three-line hybrid rice production.ConclusionWe have successfully developed a new restorer line WH6725 with disease resistance to rice blast and bacterial blight, tolerance to submergence and aromatic fragrance, which can be used to replace MH725 for hybrid rice production.

show abstract

“…With development of next generation sequencing technology, large numbers of SNPs became available, and two breeding chips RICE6K and RiceSNP50 with high-throughput SNP arrays were developed in China3031, making efficient whole-genome background selection a reality. Background selection with high resolution SNP markers was used in rice breeding to improve blast resistance and wide compatibility1632. In the present study, the breeding chip RICE6K was employed in background selection for improving BPH resistance.…”

Section: Discussionmentioning

confidence: 99%

“…There are three aspects of MABB, namely, positive selection for the target gene using linked markers, negative selection of alleles from the donor parent surrounding the target gene, and background selection for the maximum recovery of the recurrent parent genome using polymorphic markers covering the whole genome1516. MABB has been widely used in rice breeding for disease and insect resistance161718. Likewise, MABB has been used to develop multiple BPH-resistant introgression lines (ILs) and near-isogenic lines (NILs).…”

mentioning

confidence: 99%

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Brown planthopper (BPH) is the most destructive pest of rice in Asia. To date 29 BPH resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (MAS) and little knowledge of the real effects of the genes. In this study we individually transferred 13 genes or QTLs (Bph14, QBph3, QBph4, Bph17, Bph15, Bph20, Bph24, Bph6, Bph3, Bph9, Bph10, Bph18 and Bph21) into cultivar 9311 by marker assisted backcross breeding (MABB). Through positive and negative selection we narrowed the segments from donors containing Bph14, Bph15, Bph6 and Bph9 to 100–400 kb. Whole-genome background selection based on a high resolution SNP array was performed to maximize reconstitution of the recurrent parent genome (RPG 99.2–99.9%). All genes reduced BPH growth and development and showed antibiotic responses in seedlings. Based on genetic effects and amino acid sequences of genes in three clusters we inferred that Bph10 and Bph21 might be identical to Bph26, whereas Bph9 and Bph18 were different. Bph15 might be same with Bph17, but QBph4, Bph20 and Bph24 might be different. We believe that these NILs will be useful in rice BPH resistance research and breeding.

show abstract

Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice

Cited by 105 publications

References 21 publications

From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network

From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network

Marker-assisted breeding of the rice restorer line Wanhui 6725 for disease resistance, submergence tolerance and aromatic fragrance

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Contact Info

Product

Resources

About